FastQCFastQC Report
Fri 10 Feb 2017
ERR1630411.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630411.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2650577
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56200.21202930531729505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50750.19146774457033316No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41380.15611695113931795No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG27380.10329826298198469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22150.018.6252821
TCTAGCG4050.017.35802528
AAGACGG4500.017.2666665
ATACGAC650.001580670517.0769233
CAAGACG5700.016.228074
TTACTCG1504.6784407E-916.03333319
CTAGCGG4400.015.55681829
CGCAATA4650.015.11827936
TCTAATA2450.015.1020412
GACGGAC5100.014.8725497
CGCAAGA5700.014.6052632
GTATTAG6500.014.5153851
GCTTAGG10000.014.4299991
CTTATAC10400.014.40865437
TTAGACT2201.8189894E-1114.2954544
AGACGGA5450.014.2568816
GCAATAC5100.014.147058537
GTGTACG2101.364242E-1014.0952381
ACGGACC5450.013.9174318
CTAGATA4400.013.8753
Produced by FastQC (version 0.11.5)