##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630411.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2650577
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8492411274979	31.0	31.0	34.0	30.0	34.0
2	32.04604808688825	33.0	31.0	34.0	30.0	34.0
3	32.191852943717535	34.0	31.0	34.0	30.0	34.0
4	35.84031401464662	37.0	35.0	37.0	35.0	37.0
5	35.74687020976942	37.0	35.0	37.0	35.0	37.0
6	35.80397852995782	37.0	35.0	37.0	35.0	37.0
7	35.78013541957091	37.0	35.0	37.0	35.0	37.0
8	35.75318279755691	37.0	35.0	37.0	33.0	37.0
9	37.46280828664853	39.0	37.0	39.0	35.0	39.0
10	37.369380327377776	39.0	37.0	39.0	34.0	39.0
11	37.45125608499583	39.0	37.0	39.0	35.0	39.0
12	37.32834737493006	39.0	37.0	39.0	34.0	39.0
13	37.362117380479795	39.0	37.0	39.0	34.0	39.0
14	38.596435794923146	40.0	38.0	41.0	34.0	41.0
15	38.590447664791476	40.0	38.0	41.0	34.0	41.0
16	38.568009908785896	40.0	38.0	41.0	34.0	41.0
17	38.53043205309636	40.0	38.0	41.0	34.0	41.0
18	38.494482522107454	40.0	38.0	41.0	34.0	41.0
19	38.51040962024495	40.0	38.0	41.0	34.0	41.0
20	38.457087645444744	40.0	38.0	41.0	34.0	41.0
21	38.41639160077221	40.0	38.0	41.0	34.0	41.0
22	38.3905394938536	40.0	38.0	41.0	34.0	41.0
23	38.33849610858315	40.0	38.0	41.0	34.0	41.0
24	38.322943645855226	40.0	38.0	41.0	34.0	41.0
25	38.28990895189991	40.0	38.0	41.0	34.0	41.0
26	38.127037622374296	40.0	38.0	41.0	33.0	41.0
27	38.03871383476126	40.0	38.0	41.0	33.0	41.0
28	37.94878737723899	40.0	37.0	41.0	33.0	41.0
29	37.889157341967426	40.0	37.0	41.0	33.0	41.0
30	37.82360369081902	40.0	37.0	41.0	33.0	41.0
31	37.724230988196155	40.0	37.0	41.0	33.0	41.0
32	37.62262480961692	40.0	37.0	41.0	32.0	41.0
33	37.55807169533275	40.0	37.0	41.0	32.0	41.0
34	37.47534291590095	40.0	37.0	41.0	32.0	41.0
35	37.37719862505409	40.0	37.0	41.0	31.0	41.0
36	37.28191635255267	39.0	36.0	41.0	31.0	41.0
37	37.223881064387115	39.0	36.0	41.0	31.0	41.0
38	37.113662421427485	39.0	36.0	41.0	31.0	41.0
39	37.02298103394091	39.0	36.0	41.0	31.0	41.0
40	36.92415500474048	39.0	36.0	41.0	31.0	41.0
41	36.79444815223251	39.0	35.0	41.0	30.0	41.0
42	36.742802793504964	39.0	35.0	41.0	30.0	41.0
43	35.53642772875491	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	6.0
11	1.0
12	4.0
13	7.0
14	4.0
15	7.0
16	30.0
17	71.0
18	153.0
19	257.0
20	464.0
21	896.0
22	1653.0
23	2826.0
24	4449.0
25	7073.0
26	10338.0
27	15260.0
28	21447.0
29	29972.0
30	40763.0
31	53563.0
32	70128.0
33	91222.0
34	121707.0
35	164062.0
36	231136.0
37	351568.0
38	644664.0
39	786843.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.69413791789486	18.48423946936837	12.367722197845978	26.453900414890796
2	18.04158113497552	20.54039554406456	35.07790945141379	26.34011386954614
3	19.480814931994054	22.542072914689896	29.867119498886467	28.109992654429583
4	14.597010386795025	15.276786903379906	34.224736727135266	35.90146598268981
5	14.490769368329989	36.49658168768536	34.33742917108237	14.67521977290228
6	35.18750822934025	34.7786161277337	15.62527706231511	14.408598580610937
7	30.09533395936055	30.40485147196252	20.748199354329262	18.75161521434767
8	27.402486326562105	34.33075137979391	19.530841775205925	18.73592051843806
9	26.757607871795464	14.415163188996209	18.895432956673208	39.93179598253512
10	17.028933700096243	26.640425839354982	32.59871341221176	23.731927048337024
11	36.555210431540004	21.48120956304986	21.08122118316125	20.882358822248893
12	21.62574413042896	25.02738083066442	29.029566015248754	24.317309023657867
13	30.09450395140379	19.005597649115646	25.304905309296803	25.59499309018376
14	23.096820050879487	20.04314532269766	24.44920483351361	32.41082979290924
15	25.28377783403387	27.317825514972778	21.760771333939743	25.637625317053608
16	25.61657329705947	26.19252336378079	23.340465113822386	24.850438225337353
17	24.2354777846484	26.51185006132627	24.797242260836036	24.455429893189297
18	24.175792667030613	25.10257200601982	26.088055544132466	24.6335797828171
19	25.05805339743007	25.088801419464517	25.88949500429529	23.963650178810123
20	25.116078499134336	24.51926505059087	25.819698880658816	24.544957569615974
21	25.100949717740704	25.092234634194742	25.03115359410423	24.775662053960325
22	25.0792186003274	24.77996300428171	25.143883765685736	24.996934629705155
23	24.229139542069518	24.996670536264368	25.60525500674004	25.168934914926073
24	24.664327804851546	25.384548345511188	25.416918655824748	24.534205193812518
25	25.146260606652817	24.65021766958666	25.422841894425254	24.780679829335273
26	24.80229776384538	25.549342652562064	25.349914377133732	24.298445206458823
27	25.3723623196006	24.639314383245612	24.8217274955604	25.166595801593388
28	24.38684105385356	25.07254080903894	25.899191006335602	24.641427130771902
29	24.33304144720187	25.24008923340088	25.647660867803502	24.77920845159375
30	24.074418513402932	26.020409895656684	25.612875988888455	24.29229560205193
31	24.98116447852675	25.19824928685339	25.169538557076443	24.651047677543417
32	24.141913251341123	25.101289266450287	25.394055709379504	25.362741772829082
33	24.01775160653699	25.009573387228517	25.710401923807535	25.262273082426955
34	24.93377856972274	24.761061459448264	25.61744103265063	24.687718938178367
35	25.132754113538297	24.89242908242243	25.917413453749884	24.057403350289388
36	24.20197564530289	25.283023281345912	25.73201985831764	24.782981215033555
37	25.06654211516964	24.415815877071296	25.701234108648798	24.816407899110267
38	24.403705306429504	24.6732692542039	26.298651199342633	24.624374240023965
39	24.55005080025972	24.52503737865378	26.00294200093036	24.92196982015614
40	24.22223538497467	24.462711326628124	26.711806523636174	24.603246764761032
41	23.494582500338606	24.602152663363487	27.22792056220212	24.675344274095792
42	24.435019242979923	24.28048685248533	26.680228493644968	24.60426541088978
43	23.852202746798152	23.92769574322874	27.02468179569958	25.19541971427353
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	238.0
1	222.5
2	207.0
3	520.0
4	833.0
5	833.0
6	1121.0
7	1409.0
8	1293.5
9	1178.0
10	1758.5
11	2339.0
12	2339.0
13	4086.5
14	5834.0
15	9487.0
16	13140.0
17	12959.0
18	12778.0
19	12778.0
20	14878.0
21	16978.0
22	17821.5
23	18665.0
24	21772.5
25	24880.0
26	24880.0
27	29489.5
28	34099.0
29	43681.5
30	53264.0
31	60859.0
32	68454.0
33	68454.0
34	77844.5
35	87235.0
36	98271.0
37	109307.0
38	124860.0
39	140413.0
40	140413.0
41	150258.5
42	160104.0
43	159074.5
44	158045.0
45	169998.0
46	181951.0
47	181951.0
48	189143.5
49	196336.0
50	203066.5
51	209797.0
52	216274.5
53	222752.0
54	222752.0
55	207533.5
56	192315.0
57	181499.5
58	170684.0
59	156483.5
60	142283.0
61	142283.0
62	131836.0
63	121389.0
64	106362.5
65	91336.0
66	79788.0
67	68240.0
68	68240.0
69	59111.5
70	49983.0
71	43661.0
72	37339.0
73	30479.0
74	23619.0
75	23619.0
76	18550.0
77	13481.0
78	11097.5
79	8714.0
80	6647.0
81	4580.0
82	4580.0
83	3385.5
84	2191.0
85	1910.0
86	1629.0
87	1487.5
88	1346.0
89	1346.0
90	1172.5
91	999.0
92	569.0
93	139.0
94	95.0
95	51.0
96	51.0
97	30.5
98	10.0
99	11.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2650577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.241016138436215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.05513587586506	38.137000856138734
2	11.511520806002945	11.106549219606647
3	3.6887331275680606	5.338447030121853
4	1.7184854596486037	3.316059391703051
5	0.9541674428398882	2.3015003504404734
6	0.6272999065913116	1.8156950950506598
7	0.42443920423112297	1.4332764950769041
8	0.3082270784109541	1.1895349971140703
9	0.21558465099356774	0.9360020365001857
>10	1.2411563724518309	11.099832154381227
>50	0.11778411045214772	3.9738733548389598
>100	0.12128201638744755	12.158347818929883
>500	0.011727498954502811	3.8961827643051783
>1k	0.004300082949983565	2.8943212053007024
>5k	1.563666527266751E-4	0.40337723049154944
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5620	0.21202930531729505	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5075	0.19146774457033316	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4138	0.15611695113931795	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2738	0.10329826298198469	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.7727634398095206E-5	0.0
5	0.0	0.0	0.0	3.7727634398095206E-5	0.0
6	0.0	0.0	0.0	3.7727634398095206E-5	0.0
7	0.0	0.0	0.0	3.7727634398095206E-5	0.0
8	0.0	0.0	0.0	3.7727634398095206E-5	0.0
9	0.0	0.0	0.0	7.545526879619041E-5	0.0
10	0.0	0.0	0.0	1.1318290319428563E-4	0.0
11	0.0	0.0	0.0	1.5091053759238083E-4	0.0
12	0.0	0.0	0.0	2.2636580638857125E-4	0.0
13	0.0	0.0	0.0	2.2636580638857125E-4	0.0
14	0.0	0.0	0.0	2.2636580638857125E-4	0.0
15	0.0	0.0	0.0	2.6409344078666647E-4	0.0
16	0.0	0.0	0.0	5.281868815733329E-4	0.0
17	0.0	0.0	0.0	7.545526879619042E-4	0.0
18	0.0	0.0	0.0	8.300079567580945E-4	0.0
19	0.0	0.0	0.0	9.809184943504754E-4	0.0
20	0.0	0.0	0.0	0.001094101397544761	0.0
21	0.0	0.0	0.0	0.0013204672039333323	0.0
22	0.0	0.0	0.0	0.0016222882791180938	0.0
23	0.0	0.0	0.0	0.002376840967079998	0.0
24	0.0	0.0	0.0	0.003206848923838093	0.0
25	0.0	0.0	0.0	0.004036856880596187	0.0
26	0.0	0.0	0.0	0.005093230643742853	0.0
27	0.0	0.0	0.0	0.012299208813779038	0.0
28	0.0	0.0	0.0	0.035916707946986634	0.0
29	0.0	0.0	0.0	0.06745701030379422	0.0
30	0.0	0.0	0.0	0.11446564276382086	0.0
31	0.0	0.0	0.0	0.267488927882495	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2215	0.0	18.625282	1
TCTAGCG	405	0.0	17.358025	28
AAGACGG	450	0.0	17.266666	5
ATACGAC	65	0.0015806705	17.076923	3
CAAGACG	570	0.0	16.22807	4
TTACTCG	150	4.6784407E-9	16.033333	19
CTAGCGG	440	0.0	15.556818	29
CGCAATA	465	0.0	15.118279	36
TCTAATA	245	0.0	15.102041	2
GACGGAC	510	0.0	14.872549	7
CGCAAGA	570	0.0	14.605263	2
GTATTAG	650	0.0	14.515385	1
GCTTAGG	1000	0.0	14.429999	1
CTTATAC	1040	0.0	14.408654	37
TTAGACT	220	1.8189894E-11	14.295454	4
AGACGGA	545	0.0	14.256881	6
GCAATAC	510	0.0	14.1470585	37
GTGTACG	210	1.364242E-10	14.095238	1
ACGGACC	545	0.0	13.917431	8
CTAGATA	440	0.0	13.875	3
>>END_MODULE