FastQCFastQC Report
Fri 10 Feb 2017
ERR1630410.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630410.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710801
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG12490.17571725419632217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10680.15025302440486155No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT8850.12450742190852292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8840.12436673555608392No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG8490.1194427132207186No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC7910.11128290477925608No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA7810.10987604125486598No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA7320.10298240998535454No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT7230.10171623281340347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG806.952432E-720.812528
TAACACC450.003824392320.5555574
TACACGG909.465475E-820.5555575
CGCAAGA1351.8189894E-1220.5555552
TACACCG555.140439E-420.1818185
TATACTG759.257617E-619.7333345
GGTATCA4450.019.5393261
TTGCGCG1153.0449883E-919.30434818
TAATACT1457.2759576E-1219.137934
CTATACT1002.871093E-718.54
GACGGAC1409.458745E-1118.57
TTAGTAC609.2320866E-418.53
TACTAGG500.00703230418.52
TCTATAC500.00703230418.53
CTAGCGG902.1498654E-618.529
ATTTGCG1156.394657E-817.69565216
GCGCAAG1502.5102054E-1017.2666661
GATGCGT752.0658688E-417.2666666
GCAAGAC2600.017.0769233
GTTCTAA650.001579132517.0769231
Produced by FastQC (version 0.11.5)