FastQCFastQC Report
Fri 10 Feb 2017
ERR1630409.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630409.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813506
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG31550.17397240483351034No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG26490.14607064989032295No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA21710.11971286557640283No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT21160.11668006612605639No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT19180.10576198810480913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18950.10449372651648245No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC18600.10256376322989834No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAG609.239623E-418.512
ACGTGCG500.007036410718.59
GGTATCA14200.017.9788741
TCGCACG852.7245202E-517.41176422
CTTATAC5750.016.73043437
TAACGCT803.3839134E-416.18754
CGAATTA803.3839134E-416.187515
CGTGCGC700.00259326915.85714210
TCGCCAT6900.015.55072513
CTAGTAC1201.9369036E-615.4166663
TCTTATA9750.014.80000137
ACTTTTG7400.014.530
CCGATCG908.277266E-414.38888818
TGTACGA908.277266E-414.3888882
CGATCGC908.277266E-414.38888819
CTCTTAT16400.014.21341437
TAGATAC950.001245559113.6315793
AACTTTT8150.013.61963229
AGGTCGC8050.013.55900710
TTGGCCG7650.013.54248331
Produced by FastQC (version 0.11.5)