FastQCFastQC Report
Fri 10 Feb 2017
ERR1630408.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630408.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478401
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38720.26190458475068673No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG34540.23363079435146486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32760.22159075920538474No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG25940.17545983802770695No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT25280.170995555333093No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA24960.1688310546326741No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA23270.15739978530858678No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC23220.15706158207414633No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22990.15550584719572025No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT22090.14941818897579207No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC22030.14901234509446354No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT19090.1291259949093649No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA18750.12682621291516982No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC18580.1256763219180723No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC17280.11688303782262052No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA17130.11586842811929915No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA16730.11316280224377553No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT16150.10923964472426628No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA15930.10775155049272829No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC15860.10727806596451164No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG15820.1070075033769593No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC15740.10646637820185459No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC15410.10423423685454758No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC15360.10389603362010713No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC14940.10105512645080732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA1450.024.24137915
GACGGAC2450.021.1428577
CGCAAGA2650.020.9433962
ACGGACC2500.020.728
CGGACCA2600.020.6346159
CCGAATT1800.020.55555714
GCGAAAG2650.019.5471718
GCGCAAG3050.018.803281
TAGAACG500.007035813818.49999826
TCGATTT500.007035813818.49999815
AGCGAAA2850.018.17543817
AAGACGG3100.017.9032275
CGAAAGC2900.017.86206819
TAGACGT650.001580223617.0769234
AGACGGA3050.016.9836066
GAGCGAA3050.016.98360616
CGACGGT803.3833855E-416.18757
TCGCCAT5500.016.14545413
GTATAGA1504.6729838E-916.0333331
ATAGACT1059.346635E-615.8571444
Produced by FastQC (version 0.11.5)