FastQCFastQC Report
Fri 10 Feb 2017
ERR1630407.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630407.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936600
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG21540.22998078155028828No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG17340.18513773222293403No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT13510.14424514200298955No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA10450.11157377749306001No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT10160.10847747170617127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA851.9754225E-923.94117715
CAAGACG2000.022.1999994
CCTACAC957.1413524E-921.4210533
AAGACGG2000.021.2749985
CGGACCA2150.020.6511639
TTAGATC450.003825286920.55555520
GACGAGA656.900388E-519.92307732
GGTATCA4650.019.4946231
CGCAAGA2250.018.9111122
TCCATAC902.1513497E-618.53
ATTAGAC609.235072E-418.53
ACGGACC2400.018.58
CCGAATT1103.8493454E-818.514
CACACCG609.235072E-418.55
GCACTAT500.007033934418.4999986
GACGGAC2450.018.1224487
CGTTTAG1054.7949106E-717.61904726
ACGAACG1107.80381E-716.81818215
CGAACGA1005.875454E-616.6516
GTTCAAA2800.016.5178571
Produced by FastQC (version 0.11.5)