##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630407.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	936600
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.7341800128123	31.0	30.0	33.0	27.0	34.0
2	30.977296604740552	31.0	31.0	34.0	27.0	34.0
3	31.096713645099296	31.0	31.0	34.0	27.0	34.0
4	34.94752829382874	35.0	35.0	37.0	32.0	37.0
5	34.42293294896434	35.0	35.0	37.0	30.0	37.0
6	34.52665278667521	35.0	35.0	37.0	30.0	37.0
7	34.54329169335896	35.0	35.0	37.0	30.0	37.0
8	34.466386931454196	35.0	35.0	37.0	30.0	37.0
9	36.06183642963912	37.0	35.0	39.0	32.0	39.0
10	35.7499135169763	37.0	35.0	39.0	30.0	39.0
11	35.954396754217385	37.0	35.0	39.0	31.0	39.0
12	35.75555947042494	37.0	35.0	39.0	30.0	39.0
13	35.855289344437324	37.0	35.0	39.0	30.0	39.0
14	36.78463484945548	38.0	36.0	40.0	31.0	41.0
15	36.73025624599616	38.0	36.0	40.0	31.0	41.0
16	36.67324151185138	38.0	36.0	40.0	31.0	41.0
17	36.58496049540893	38.0	36.0	40.0	31.0	41.0
18	36.50518150758061	38.0	35.0	40.0	31.0	41.0
19	36.50498398462524	38.0	35.0	40.0	30.0	41.0
20	36.42966794789665	38.0	35.0	40.0	30.0	41.0
21	36.359553704890025	38.0	35.0	40.0	30.0	41.0
22	36.32927290198591	38.0	35.0	40.0	30.0	41.0
23	36.24325325645953	38.0	35.0	40.0	30.0	41.0
24	36.204864403160364	38.0	35.0	40.0	30.0	41.0
25	36.138793508434766	38.0	35.0	40.0	30.0	41.0
26	35.90524770446295	38.0	34.0	40.0	29.0	41.0
27	35.77875507153534	38.0	34.0	40.0	29.0	41.0
28	35.660517830450566	38.0	34.0	40.0	28.0	41.0
29	35.54867392696989	38.0	34.0	40.0	27.0	41.0
30	35.4723798846893	38.0	34.0	40.0	27.0	41.0
31	35.328211616485156	38.0	34.0	40.0	27.0	41.0
32	35.19154601751014	38.0	34.0	40.0	27.0	41.0
33	35.13063100576554	38.0	34.0	40.0	27.0	40.0
34	35.00219837710869	38.0	33.0	40.0	26.0	40.0
35	34.88668054665813	38.0	33.0	40.0	26.0	40.0
36	34.77426542814435	38.0	33.0	40.0	25.0	40.0
37	34.71309096732863	37.0	33.0	40.0	25.0	40.0
38	34.55708306641042	37.0	33.0	39.0	25.0	40.0
39	34.434762972453555	37.0	33.0	39.0	25.0	40.0
40	34.31765748451847	37.0	33.0	39.0	24.0	40.0
41	34.16519431988042	37.0	32.0	39.0	24.0	40.0
42	34.126844971172325	37.0	32.0	39.0	24.0	40.0
43	32.772134315609655	36.0	30.0	38.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	2.0
12	2.0
13	3.0
14	13.0
15	32.0
16	53.0
17	125.0
18	230.0
19	383.0
20	719.0
21	1220.0
22	1996.0
23	3089.0
24	4948.0
25	6975.0
26	10147.0
27	13597.0
28	18227.0
29	23944.0
30	30942.0
31	39148.0
32	49685.0
33	63021.0
34	81510.0
35	107254.0
36	139783.0
37	165911.0
38	137613.0
39	36024.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.4096732863549	19.85532778133675	13.421204356181935	26.313794576126416
2	19.39760837070254	21.320093956865257	34.11306854580397	25.169229126628228
3	20.642750373692078	22.683002348921633	29.834187486653857	26.840059790732436
4	16.358210548793508	15.979286781977365	33.26286568439035	34.39963698483878
5	14.91693358957933	36.82842195174034	33.8168908819133	14.43775357676703
6	36.070360879777915	34.66666666666667	14.935724962630792	14.327247490924622
7	30.139120222079864	30.460068332265642	20.391842835789024	19.00896860986547
8	26.85799701046338	35.10783685671578	19.36995515695067	18.66421097587017
9	26.94875080076874	14.449498184924195	18.50192184497117	40.09982916933589
10	16.846465940636342	27.509075379030534	32.38137945761264	23.263079222720478
11	37.00822122571002	21.226137091607946	20.553384582532562	21.212257100149476
12	21.732329703181723	25.284966901558832	28.22848601323938	24.754217382020073
13	30.46465940636344	18.9629510997224	24.914904975443093	25.657484518471062
14	23.251121076233183	20.302370275464448	23.67403373905616	32.772474909246206
15	25.874973307708732	27.748878923766817	21.237774930600043	25.138372837924404
16	26.17168481742473	26.352551783045058	22.562353192398035	24.91341020713218
17	24.72400170830664	26.297992739696774	24.13025838137946	24.847747170617126
18	24.225816784112748	25.03373905616058	25.613388853299167	25.127055306427504
19	25.654388212684175	25.226564168268204	24.97266709374333	24.14638052530429
20	25.83290625667307	24.413089899636987	25.118727311552426	24.635276532137517
21	25.593423019431988	25.324898569293186	24.077941490497544	25.003736920777282
22	25.531817211189406	24.74418108050395	24.34785393978219	25.376147768524447
23	24.450565876574846	25.330023489216313	24.778774289985055	25.440636344223787
24	25.754644458680332	25.403694213111255	24.46711509715994	24.374546231048473
25	25.774076446722187	24.80621396540679	24.617552850736708	24.802156737134315
26	25.692504804612426	25.451099722400173	24.160153747597693	24.696241725389708
27	25.882020072603034	24.471812940422804	24.294789664744822	25.35137732222934
28	24.965193252188765	24.763613068545805	25.033205210335257	25.237988468930173
29	24.59182148195601	25.276105060858423	24.96796925048046	25.1641042067051
30	24.601537475976937	26.25048046124279	24.54815289344437	24.599829169335898
31	25.772795216741407	25.21012171684817	24.301729660474056	24.715353405936366
32	24.479073243647235	25.11242793081358	24.828101644245145	25.58039718129404
33	24.649370061926117	24.826500106769164	24.942344650864833	25.581785180439887
34	25.381272688447577	24.579222720478324	25.08413410207132	24.955370489002775
35	25.633781763826608	24.470318172111895	25.22998078155029	24.66591928251121
36	24.765321375186847	25.324898569293186	24.92686312193039	24.98291693358958
37	25.994234465086485	23.786141362374547	25.02711936792654	25.192504804612426
38	24.998291693358958	24.24610292547512	26.002882767456757	24.75272261370916
39	25.223681400811444	23.863335468716635	25.83888532991672	25.0740978005552
40	24.424193892803757	23.751548152893445	26.72538970745249	25.09886824685031
41	24.041746743540465	23.96818278881059	27.01398676062353	24.976083707025413
42	24.94981849241939	23.445547725816784	26.617979927396966	24.986653854366857
43	24.091394405295752	23.197202647875294	26.930706811872728	25.780696134956226
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	10.0
2	15.0
3	33.5
4	52.0
5	52.0
6	85.0
7	118.0
8	117.5
9	117.0
10	177.0
11	237.0
12	237.0
13	430.0
14	623.0
15	1209.5
16	1796.0
17	1860.5
18	1925.0
19	1925.0
20	2644.0
21	3363.0
22	4112.0
23	4861.0
24	6124.0
25	7387.0
26	7387.0
27	8766.5
28	10146.0
29	13894.5
30	17643.0
31	20028.5
32	22414.0
33	22414.0
34	25814.5
35	29215.0
36	33063.5
37	36912.0
38	43460.5
39	50009.0
40	50009.0
41	53689.5
42	57370.0
43	56405.0
44	55440.0
45	60484.5
46	65529.0
47	65529.0
48	70217.5
49	74906.0
50	77514.5
51	80123.0
52	84981.5
53	89840.0
54	89840.0
55	79831.5
56	69823.0
57	65671.5
58	61520.0
59	55627.5
60	49735.0
61	49735.0
62	46011.5
63	42288.0
64	37497.0
65	32706.0
66	28453.0
67	24200.0
68	24200.0
69	20701.5
70	17203.0
71	14811.0
72	12419.0
73	9982.0
74	7545.0
75	7545.0
76	5672.5
77	3800.0
78	3139.0
79	2478.0
80	1862.0
81	1246.0
82	1246.0
83	954.0
84	662.0
85	523.0
86	384.0
87	315.5
88	247.0
89	247.0
90	244.0
91	241.0
92	139.5
93	38.0
94	25.0
95	12.0
96	12.0
97	8.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	936600.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.487348669531926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.65588894235908	52.43735236848587
2	5.868234233633624	6.864349233940263
3	1.5337523990816464	2.69115334013458
4	0.6938887279633807	1.6233484788100894
5	0.3831568134311685	1.1204913071127771
6	0.2556269174002157	0.897056438838243
7	0.20066049660410418	0.8215270300359968
8	0.1488575519462418	0.6965026834218245
9	0.11322572118838639	0.5960045010153927
>10	0.8848032669811742	10.587739952779526
>50	0.1375576259744358	5.661219654402803
>100	0.11682777625602453	12.85602328324186
>500	0.006602511670748015	2.364178162245698
>1k	9.170155098261132E-4	0.7830535655350995
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2154	0.22998078155028828	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1734	0.18513773222293403	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	1351	0.14424514200298955	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1045	0.11157377749306001	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1016	0.10847747170617127	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.0676916506512919E-4	0.0
11	0.0	1.0676916506512919E-4	0.0	1.0676916506512919E-4	0.0
12	0.0	1.0676916506512919E-4	0.0	1.0676916506512919E-4	0.0
13	0.0	1.0676916506512919E-4	0.0	1.0676916506512919E-4	0.0
14	0.0	1.0676916506512919E-4	0.0	1.0676916506512919E-4	0.0
15	0.0	1.0676916506512919E-4	0.0	2.1353833013025838E-4	0.0
16	0.0	1.0676916506512919E-4	0.0	2.1353833013025838E-4	0.0
17	0.0	1.0676916506512919E-4	0.0	3.2030749519538755E-4	0.0
18	0.0	1.0676916506512919E-4	0.0	4.2707666026051675E-4	0.0
19	0.0	1.0676916506512919E-4	0.0	4.2707666026051675E-4	0.0
20	0.0	1.0676916506512919E-4	0.0	4.2707666026051675E-4	0.0
21	0.0	1.0676916506512919E-4	0.0	5.33845825325646E-4	0.0
22	0.0	1.0676916506512919E-4	0.0	9.609224855861628E-4	0.0
23	0.0	1.0676916506512919E-4	0.0	0.0014947683109118087	0.0
24	0.0	1.0676916506512919E-4	0.0	0.0025624599615631004	0.0
25	0.0	1.0676916506512919E-4	0.0	0.0029895366218236174	0.0
26	0.0	1.0676916506512919E-4	0.0	0.00395045910740978	0.0
27	0.0	1.0676916506512919E-4	0.0	0.007260303224428785	0.0
28	0.0	2.1353833013025838E-4	0.0	0.019538757206918642	0.0
29	0.0	2.1353833013025838E-4	0.0	0.04238735853085629	0.0
30	0.0	2.1353833013025838E-4	0.0	0.07548579970104634	0.0
31	0.0	2.1353833013025838E-4	0.0	0.2109758701686953	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTA	85	1.9754225E-9	23.941177	15
CAAGACG	200	0.0	22.199999	4
CCTACAC	95	7.1413524E-9	21.421053	3
AAGACGG	200	0.0	21.274998	5
CGGACCA	215	0.0	20.651163	9
TTAGATC	45	0.0038252869	20.555555	20
GACGAGA	65	6.900388E-5	19.923077	32
GGTATCA	465	0.0	19.494623	1
CGCAAGA	225	0.0	18.911112	2
TCCATAC	90	2.1513497E-6	18.5	3
ATTAGAC	60	9.235072E-4	18.5	3
ACGGACC	240	0.0	18.5	8
CCGAATT	110	3.8493454E-8	18.5	14
CACACCG	60	9.235072E-4	18.5	5
GCACTAT	50	0.0070339344	18.499998	6
GACGGAC	245	0.0	18.122448	7
CGTTTAG	105	4.7949106E-7	17.619047	26
ACGAACG	110	7.80381E-7	16.818182	15
CGAACGA	100	5.875454E-6	16.65	16
GTTCAAA	280	0.0	16.517857	1
>>END_MODULE