FastQCFastQC Report
Fri 10 Feb 2017
ERR1630406.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630406.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2811618
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61550.21891309559122185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61530.21884196217267066No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46640.16588313206132554No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG41830.14877554489976946No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT37050.13177465786604012No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC32010.11384903639114559No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA31330.11143050016040586No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG30570.10872743025546144No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30520.10854959670908353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24500.017.5183681
CTTATAC13250.017.03396237
CGAACGA1059.352638E-615.85714216
CGACTAT700.002593744815.8571426
ATCTCGC10400.015.29807711
TTCCTCG11100.015.019
GCTTAGG14250.014.9298241
ATACCGA1001.0942577E-414.7999996
TTAGGAC15850.014.5899053
TCTCGCC11500.014.47826112
TAGGACA15900.014.1949684
CGGCCTT11600.014.03448324
CTTAGGA15450.014.0097092
GACGGAC3050.013.950827
TCTTATA21000.013.91904837
TCCTCGG12550.013.85657320
ACGGACC2950.013.1694928
TAACTCG850.00940915613.0588235
GTCTATA1857.3949195E-813.01
ATACCGT2850.012.9824566
Produced by FastQC (version 0.11.5)