##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630406.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2811618 Sequences flagged as poor quality 0 Sequence length 43 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00967450059005 33.0 31.0 34.0 30.0 34.0 2 32.23705211732177 34.0 31.0 34.0 30.0 34.0 3 32.36239844815334 34.0 31.0 34.0 30.0 34.0 4 35.97352591995072 37.0 35.0 37.0 35.0 37.0 5 35.89863985790388 37.0 35.0 37.0 35.0 37.0 6 35.95151759591808 37.0 35.0 37.0 35.0 37.0 7 35.937971303356285 37.0 35.0 37.0 35.0 37.0 8 35.94344146324287 37.0 35.0 37.0 35.0 37.0 9 37.71274049319644 39.0 37.0 39.0 35.0 39.0 10 37.623654066804235 39.0 37.0 39.0 35.0 39.0 11 37.67431742149894 39.0 37.0 39.0 35.0 39.0 12 37.61738827963116 39.0 37.0 39.0 35.0 39.0 13 37.61732283688609 39.0 37.0 39.0 35.0 39.0 14 38.9092536752859 40.0 38.0 41.0 36.0 41.0 15 38.92859449612288 40.0 38.0 41.0 36.0 41.0 16 38.88565943168667 40.0 38.0 41.0 36.0 41.0 17 38.86379586416078 40.0 38.0 41.0 35.0 41.0 18 38.81931649320783 40.0 38.0 41.0 35.0 41.0 19 38.85537082206758 40.0 38.0 41.0 35.0 41.0 20 38.83312100007896 40.0 38.0 41.0 35.0 41.0 21 38.78675445953184 40.0 38.0 41.0 35.0 41.0 22 38.76144874588226 40.0 38.0 41.0 35.0 41.0 23 38.71720269254216 40.0 38.0 41.0 35.0 41.0 24 38.69342599172434 40.0 38.0 41.0 34.0 41.0 25 38.67235164947728 40.0 38.0 41.0 34.0 41.0 26 38.53630329582468 40.0 38.0 41.0 34.0 41.0 27 38.43432500432136 40.0 38.0 41.0 34.0 41.0 28 38.38599767109188 40.0 38.0 41.0 34.0 41.0 29 38.34435083286563 40.0 38.0 41.0 34.0 41.0 30 38.2902961924415 40.0 38.0 41.0 34.0 41.0 31 38.23990705707532 40.0 38.0 41.0 34.0 41.0 32 38.16311212974166 40.0 38.0 41.0 34.0 41.0 33 38.095661999603074 40.0 38.0 41.0 33.0 41.0 34 38.04293506443621 40.0 38.0 41.0 33.0 41.0 35 37.9387121579105 40.0 37.0 41.0 33.0 41.0 36 37.86981446270439 40.0 37.0 41.0 33.0 41.0 37 37.81539633051147 40.0 37.0 41.0 33.0 41.0 38 37.72036350599548 40.0 37.0 41.0 33.0 41.0 39 37.648966182461486 40.0 37.0 41.0 33.0 41.0 40 37.56789649234 40.0 37.0 41.0 33.0 41.0 41 37.462723599009536 40.0 37.0 41.0 32.0 41.0 42 37.43402908930018 40.0 37.0 41.0 32.0 41.0 43 36.28792780527085 38.0 35.0 40.0 30.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 1.0 9 9.0 10 15.0 11 7.0 12 2.0 13 6.0 14 2.0 15 5.0 16 15.0 17 25.0 18 72.0 19 127.0 20 264.0 21 568.0 22 1025.0 23 1778.0 24 3122.0 25 4792.0 26 7429.0 27 11372.0 28 16435.0 29 23211.0 30 32865.0 31 44500.0 32 59625.0 33 79382.0 34 109123.0 35 152191.0 36 218685.0 37 346461.0 38 670901.0 39 1027602.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.98248126167922 19.49094791682227 12.66921751105591 25.857353310442598 2 17.940630626208822 21.416458423583858 35.77345855660335 24.869452393603968 3 19.542982012492452 23.546655342226433 30.515062857045304 26.395299788235814 4 14.963483659586757 15.941710431502432 34.71015621610048 34.384649692810335 5 14.498697902773422 36.74727505656885 34.427792111161615 14.326234929496112 6 33.9519095410543 35.210259715224474 16.156995722747542 14.680835020973687 7 28.425411986976894 30.794119258021535 21.963687812498 18.81678094250357 8 26.032483786915577 34.852067386110065 20.453383069819584 18.662065757154778 9 26.188977307728152 15.035257278904886 20.14096509554285 38.634800317824116 10 16.658664157079663 27.33177124346195 33.65318474984866 22.356379849609727 11 34.69703210037779 22.4063866428512 22.25437452740735 20.642206729363664 12 20.71476281628585 25.899677694480545 29.951864015666423 23.433695473567177 13 29.266280127670257 20.396405201560096 25.700824222920755 24.636490447848892 14 22.379747177603786 21.348810542541695 25.407434438106456 30.86400784174806 15 24.729390692476716 28.238900163535728 22.979010662188106 24.052698481799446 16 24.51044203017622 27.09767116301005 24.278155851897377 24.113730954916353 17 23.506713927709953 27.120896224167012 25.75634385609994 23.6160459920231 18 22.75330432512525 26.07182056737437 27.15287069580576 24.02200441169462 19 24.066320531451996 26.12616649914747 27.283009285045125 22.524503684355413 20 24.049568611383197 25.586655086146127 27.22994375480595 23.133832547664728 21 23.625969103910986 26.3657438528278 26.119373257675832 23.88891378558538 22 23.915695517669896 25.856179609036506 26.266832834332405 23.961292038961197 23 23.324683509637513 26.362934082795032 26.463196636242902 23.849185771324557 24 24.32766471120899 26.294468167439533 26.22834965489622 23.149517466455258 25 24.30429738321493 25.479919391610096 26.601373301778548 23.61440992339642 26 24.016811672140385 26.051903210180043 26.316519527190398 23.614765590489178 27 24.02506314869232 25.790523463713775 26.047030571009287 24.137382816584616 28 23.359681151564686 26.173505789193268 26.738518532745204 23.728294526496843 29 23.5583567895781 26.484999029028838 26.54877013875996 23.407874042633104 30 23.128106307471356 27.178585426612006 26.352192936593806 23.341115329322832 31 23.869067561809608 26.12403249659093 26.56801172847805 23.438888213121412 32 23.10157354235177 26.301190275492615 26.537210958245396 24.060025223910216 33 23.264860304635977 26.07363446954743 26.679335528510627 23.982169697305963 34 23.63311801247538 25.85326313887591 26.60894901085425 23.904669837794465 35 23.879524174336627 25.907431237102625 26.6942379796971 23.518806608863652 36 23.414489450558364 26.07704887363788 26.7034141906902 23.805047485113555 37 24.11013871727952 24.86564675571148 26.769497136524233 24.254717390484767 38 23.33425095443264 25.546606971501816 27.474784981459077 23.644357092606462 39 23.193904719631185 25.26776397078124 27.736698228564478 23.801633081023095 40 23.160080779110107 24.7358282668556 28.272973071021738 23.831117883012556 41 22.393547060802714 25.36884455854245 28.4730358106969 23.764572569957938 42 22.957172702692898 24.665370615780667 28.106094071100696 24.27136261042574 43 22.33685372621743 24.852309239733135 28.28922705716068 24.521609976888755 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 509.0 1 464.0 2 419.0 3 930.5 4 1442.0 5 1442.0 6 1898.0 7 2354.0 8 2266.0 9 2178.0 10 3230.5 11 4283.0 12 4283.0 13 6921.0 14 9559.0 15 14352.5 16 19146.0 17 18956.0 18 18766.0 19 18766.0 20 21816.0 21 24866.0 22 28170.0 23 31474.0 24 35601.0 25 39728.0 26 39728.0 27 44842.0 28 49956.0 29 65184.5 30 80413.0 31 84968.0 32 89523.0 33 89523.0 34 99226.5 35 108930.0 36 117574.0 37 126218.0 38 144203.5 39 162189.0 40 162189.0 41 174705.5 42 187222.0 43 180116.0 44 173010.0 45 182747.0 46 192484.0 47 192484.0 48 199857.5 49 207231.0 50 212965.0 51 218699.0 52 223652.5 53 228606.0 54 228606.0 55 202466.0 56 176326.0 57 164894.0 58 153462.0 59 139315.5 60 125169.0 61 125169.0 62 115736.5 63 106304.0 64 94911.5 65 83519.0 66 72872.0 67 62225.0 68 62225.0 69 53624.0 70 45023.0 71 38765.0 72 32507.0 73 26566.0 74 20625.0 75 20625.0 76 16220.5 77 11816.0 78 9607.5 79 7399.0 80 5615.5 81 3832.0 82 3832.0 83 2835.5 84 1839.0 85 1471.0 86 1103.0 87 898.0 88 693.0 89 693.0 90 577.0 91 461.0 92 268.5 93 76.0 94 47.0 95 18.0 96 18.0 97 15.0 98 12.0 99 8.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 2811618.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.80764251180582 #Duplication Level Percentage of deduplicated Percentage of total 1 77.496341905014 36.27421067862572 2 12.488100731585199 11.690771093909214 3 4.072468343374163 5.718679270719117 4 1.8746665214831766 3.509948814657403 5 0.9835835178971115 2.3019612843116177 6 0.6044551790193143 1.6975873160367396 7 0.4145085150850532 1.358151647454045 8 0.29523938273341144 1.105556758591339 9 0.22850649859940797 0.9626265448258993 >10 1.2911591106217895 11.267130009666506 >50 0.12343565378428722 4.044232296486498 >100 0.1059172327157458 10.204115855098832 >500 0.016213056065537544 5.060154641810708 >1k 0.00525211675361965 4.366353655072857 >5k 1.5223526822085942E-4 0.4385201327336108 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 6155 0.21891309559122185 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 6153 0.21884196217267066 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 4664 0.16588313206132554 No Hit GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG 4183 0.14877554489976946 No Hit GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT 3705 0.13177465786604012 No Hit CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC 3201 0.11384903639114559 No Hit TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA 3133 0.11143050016040586 No Hit CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG 3057 0.10872743025546144 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3052 0.10854959670908353 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 7.113341855116875E-5 0.0 0.0 0.0 4 0.0 7.113341855116875E-5 0.0 0.0 0.0 5 0.0 1.0670012782675313E-4 0.0 0.0 0.0 6 0.0 1.0670012782675313E-4 0.0 0.0 0.0 7 0.0 1.0670012782675313E-4 0.0 0.0 0.0 8 0.0 1.0670012782675313E-4 0.0 0.0 0.0 9 0.0 1.0670012782675313E-4 0.0 7.113341855116875E-5 0.0 10 0.0 1.0670012782675313E-4 0.0 1.422668371023375E-4 0.0 11 0.0 1.0670012782675313E-4 0.0 1.422668371023375E-4 0.0 12 0.0 1.0670012782675313E-4 0.0 1.778335463779219E-4 0.0 13 0.0 1.0670012782675313E-4 0.0 2.1340025565350627E-4 0.0 14 0.0 1.0670012782675313E-4 0.0 3.201003834802594E-4 0.0 15 0.0 1.0670012782675313E-4 0.0 3.9123380203142814E-4 0.0 16 0.0 1.0670012782675313E-4 0.0 3.9123380203142814E-4 0.0 17 0.0 1.0670012782675313E-4 0.0 6.402007669605188E-4 0.0 18 0.0 1.422668371023375E-4 0.0 7.469008947872719E-4 0.0 19 0.0 1.422668371023375E-4 0.0 7.824676040628563E-4 0.0 20 0.0 1.422668371023375E-4 0.0 9.247344411651939E-4 0.0 21 0.0 1.422668371023375E-4 0.0 0.0011737014060942846 0.0 22 0.0 1.422668371023375E-4 0.0 0.0015649352081257126 0.0 23 0.0 1.778335463779219E-4 0.0 0.002169569265810647 0.0 24 0.0 2.1340025565350627E-4 0.0 0.003627804346109607 0.0 25 0.0 2.1340025565350627E-4 0.0 0.004445838659448047 0.0 26 0.0 2.1340025565350627E-4 0.0 0.007397875529321551 0.0 27 0.0 2.1340025565350627E-4 0.0 0.018245721858374787 0.0 28 0.0 2.4896696492909066E-4 0.0 0.04705475637159813 0.0 29 0.0 2.84533674204675E-4 0.0 0.08681833734170147 0.0 30 0.0 2.84533674204675E-4 0.0 0.14006170112725128 0.0 31 0.0 2.84533674204675E-4 0.0 0.3331889324936745 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2450 0.0 17.518368 1 CTTATAC 1325 0.0 17.033962 37 CGAACGA 105 9.352638E-6 15.857142 16 CGACTAT 70 0.0025937448 15.857142 6 ATCTCGC 1040 0.0 15.298077 11 TTCCTCG 1110 0.0 15.0 19 GCTTAGG 1425 0.0 14.929824 1 ATACCGA 100 1.0942577E-4 14.799999 6 TTAGGAC 1585 0.0 14.589905 3 TCTCGCC 1150 0.0 14.478261 12 TAGGACA 1590 0.0 14.194968 4 CGGCCTT 1160 0.0 14.034483 24 CTTAGGA 1545 0.0 14.009709 2 GACGGAC 305 0.0 13.95082 7 TCTTATA 2100 0.0 13.919048 37 TCCTCGG 1255 0.0 13.856573 20 ACGGACC 295 0.0 13.169492 8 TAACTCG 85 0.009409156 13.058823 5 GTCTATA 185 7.3949195E-8 13.0 1 ATACCGT 285 0.0 12.982456 6 >>END_MODULE