FastQCFastQC Report
Fri 10 Feb 2017
ERR1630405.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630405.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2408002
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100840.4187704163036409No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT84660.35157778108157717No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66000.27408615109123663No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44690.18558954685253584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29600.023.8125021
CTTATAC12300.016.39430837
GTATCAA45400.015.4030842
ACGCTTC4000.013.87499931
CGGACCA4000.013.8749999
GTAAACG4050.013.70370427
CGAATTA2850.013.63157815
AAGACGG4000.013.4124995
GCGTTAT3400.013.0588241
AACGCTT4400.013.03409130
AAATGCG2703.6379788E-1213.01851834
CGTTTAG2850.012.98245626
TCTTATA19000.012.56052637
CTCTTAT28400.012.50704337
GTCTAGG3550.012.5070431
ACGGACC5500.012.4454548
GACGGAC5500.012.4454547
ACTCTAA4950.012.33333410
CGCAAGA4800.012.3333342
CGTTATT3600.012.3333332
Produced by FastQC (version 0.11.5)