FastQCFastQC Report
Fri 10 Feb 2017
ERR1630404.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630404.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2651708
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT80060.30191861245657514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64370.2427492016466368No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57640.21736933327500615No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27390.1032919160028178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22550.019.93571
TATACGT752.0685508E-417.2666664
TAATACG1352.222987E-816.4444434
TAACGCC2400.015.4166674
ATAACGC3050.015.1639353
TCGTTTA2450.015.1020430
CTAGAAC4700.014.9574473
CGGCATA750.00410628814.828
GACGGAC2750.014.87
TCTTATA19050.014.664041537
GTCTAGA3800.014.1184221
ACTGATC3850.013.9350648
CTTATAC11650.013.81545237
CGTTTAT2551.8189894E-1213.78431331
TACACCG1501.3072258E-613.5666675
CGCATCG2601.8189894E-1213.51923113
TATACTG4600.013.2717395
GTATAGA3600.012.8472221
AAGACGG3200.012.718755
GTATCAA35300.012.6303112
Produced by FastQC (version 0.11.5)