##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630404.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2651708
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.826353806678565	33.0	31.0	34.0	30.0	34.0
2	32.040971328668164	33.0	31.0	34.0	30.0	34.0
3	32.17733136529361	34.0	31.0	34.0	30.0	34.0
4	35.818542991913134	37.0	35.0	37.0	35.0	37.0
5	35.74116531684484	37.0	35.0	37.0	35.0	37.0
6	35.794147017695764	37.0	35.0	37.0	35.0	37.0
7	35.769539481722724	37.0	35.0	37.0	33.0	37.0
8	35.7671632774046	37.0	35.0	37.0	33.0	37.0
9	37.50786662784892	39.0	37.0	39.0	35.0	39.0
10	37.38371570323731	39.0	37.0	39.0	34.0	39.0
11	37.46174767357492	39.0	37.0	39.0	35.0	39.0
12	37.3795515946703	39.0	37.0	39.0	34.0	39.0
13	37.39233920175223	39.0	37.0	39.0	34.0	39.0
14	38.6322200634459	40.0	38.0	41.0	34.0	41.0
15	38.63507143320456	40.0	38.0	41.0	34.0	41.0
16	38.60110653209177	40.0	38.0	41.0	34.0	41.0
17	38.56898421696506	40.0	38.0	41.0	34.0	41.0
18	38.53348370182539	40.0	38.0	41.0	34.0	41.0
19	38.564234071021396	40.0	38.0	41.0	34.0	41.0
20	38.54324458047417	40.0	38.0	41.0	34.0	41.0
21	38.48806090263332	40.0	38.0	41.0	34.0	41.0
22	38.45488002449742	40.0	38.0	41.0	34.0	41.0
23	38.40216004175422	40.0	38.0	41.0	34.0	41.0
24	38.378190962202474	40.0	38.0	41.0	34.0	41.0
25	38.35698689297615	40.0	38.0	41.0	34.0	41.0
26	38.19818622563269	40.0	38.0	41.0	34.0	41.0
27	38.08765595608566	40.0	38.0	41.0	33.0	41.0
28	38.02944441846538	40.0	38.0	41.0	33.0	41.0
29	37.966154267362775	40.0	37.0	41.0	33.0	41.0
30	37.90978795553658	40.0	37.0	41.0	33.0	41.0
31	37.855745806099314	40.0	37.0	41.0	33.0	41.0
32	37.775051023717545	40.0	37.0	41.0	33.0	41.0
33	37.679994177337775	40.0	37.0	41.0	33.0	41.0
34	37.620346961279296	40.0	37.0	41.0	32.0	41.0
35	37.51612583285942	40.0	37.0	41.0	32.0	41.0
36	37.43791850384733	40.0	37.0	41.0	32.0	41.0
37	37.373940117086796	40.0	37.0	41.0	31.0	41.0
38	37.27962581098673	40.0	36.0	41.0	31.0	41.0
39	37.20257245518737	39.0	36.0	41.0	31.0	41.0
40	37.11203873126302	39.0	36.0	41.0	31.0	41.0
41	37.00330466250432	39.0	36.0	41.0	31.0	41.0
42	36.96510437800844	39.0	36.0	41.0	31.0	41.0
43	35.7716147479285	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	10.0
11	4.0
12	3.0
13	5.0
14	4.0
15	8.0
16	23.0
17	42.0
18	72.0
19	158.0
20	306.0
21	671.0
22	1281.0
23	2289.0
24	4092.0
25	6283.0
26	9781.0
27	14756.0
28	21099.0
29	29644.0
30	39967.0
31	52682.0
32	68442.0
33	88520.0
34	117384.0
35	157730.0
36	222238.0
37	341291.0
38	642150.0
39	830766.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.34719659932391	19.181750026775195	12.371347071397002	26.099706302503893
2	17.62218917014996	20.76997165600436	35.74269866817915	25.865140505666535
3	19.056660838976235	23.26100762225705	30.184620629420735	27.497710909345972
4	13.786925257230434	15.62551381977201	35.802433752132586	34.78512717086497
5	14.284415931165876	36.89810491954619	34.518581985648495	14.298897163639435
6	34.05759608524016	36.425126748495686	15.806265244891218	13.711011921372943
7	29.32966224033717	31.098295890799438	21.391721863794956	18.18032000506843
8	27.43480805578895	34.81725740541568	19.581152977628005	18.16678156116737
9	26.897230011750917	14.263222044056134	19.475673792136995	39.363874152055956
10	16.192544578814864	27.315300176339175	33.46884347748696	23.023311767359
11	36.31561242791438	21.858289072552484	21.735688846584917	20.090409652948214
12	21.315318277879765	25.42018201099065	30.489782434566703	22.774717276562882
13	30.15418741430052	19.890915591007758	25.053286410117554	24.90161058457417
14	22.32413976199491	20.108096366568265	25.97933105756742	31.5884328138694
15	24.66368846041872	27.91702555484993	22.566247867412248	24.8530381173191
16	24.496136075314475	26.7926936148324	24.16966724843007	24.541503061423054
17	23.168501207523605	26.790506345344205	26.075608626590864	23.965383820541327
18	23.598299661953728	25.831577232485632	26.76927474669157	23.800848358869075
19	24.142703495256644	25.68039165699994	26.72794289567328	23.44896195207014
20	24.352869923837765	25.584076376433607	26.499109253356707	23.563944446371924
21	24.679225616093476	25.45416011114346	26.077418780650056	23.78919549211301
22	24.472151534030143	25.49858430867954	26.354108370906598	23.67515578638372
23	24.079498949356413	25.43568145512251	26.435188188141378	24.049631407379696
24	23.935252297764308	25.694194081701305	26.24787495455759	24.122678665976796
25	24.39985850629104	25.224421391797286	26.42466666767231	23.951053434239366
26	24.1207553772889	25.701510121023887	26.28690640145898	23.89082810022823
27	23.969418955631618	25.672359098362264	26.3541837939924	24.00403815201372
28	23.94509501046118	25.602253340111353	26.560843049083836	23.891808600343627
29	23.62809178084465	25.768108705785103	26.749740167469422	23.85405934590083
30	23.62722441535795	26.21717775863707	26.33909917683244	23.816498649172534
31	24.08055487255761	25.90556727965523	26.067802337210583	23.946075510576577
32	23.614628760029387	25.839308098780105	26.426967071789203	24.11909606940131
33	23.4986280540693	25.678807772198148	26.857444333991527	23.96511983974103
34	24.152169092524517	25.30108895851278	26.466752749548593	24.079989199414115
35	23.844706883261658	25.499225404908838	26.88293733699186	23.77313037483765
36	23.951845376640264	25.584038664890706	26.395553356553584	24.068562601915445
37	23.823437573066116	25.172568020309928	26.883729279392753	24.120265127231203
38	23.449527625213637	25.335481885637485	27.122556480577803	24.09243400857108
39	23.403934369847658	25.136591208383425	27.366964990112034	24.09250943165688
40	23.278618912791302	24.972508285225974	27.689247835734555	24.05962496624817
41	22.930390525653653	24.984161151982043	27.90175237997547	24.18369594238883
42	22.987334955432498	25.034204369410208	27.852425681862407	24.126034993294887
43	22.787878605034944	24.632576437526303	28.060593398669837	24.518951558768915
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	329.0
1	329.0
2	329.0
3	756.5
4	1184.0
5	1184.0
6	1621.0
7	2058.0
8	1875.0
9	1692.0
10	2358.0
11	3024.0
12	3024.0
13	5446.0
14	7868.0
15	12486.5
16	17105.0
17	17087.0
18	17069.0
19	17069.0
20	21387.0
21	25705.0
22	26428.5
23	27152.0
24	32635.5
25	38119.0
26	38119.0
27	44663.5
28	51208.0
29	58680.0
30	66152.0
31	74186.5
32	82221.0
33	82221.0
34	93108.0
35	103995.0
36	113746.5
37	123498.0
38	132221.0
39	140944.0
40	140944.0
41	151040.0
42	161136.0
43	165499.0
44	169862.0
45	173705.0
46	177548.0
47	177548.0
48	182584.0
49	187620.0
50	188312.5
51	189005.0
52	188022.0
53	187039.0
54	187039.0
55	181082.5
56	175126.0
57	166757.0
58	158388.0
59	150529.0
60	142670.0
61	142670.0
62	130383.0
63	118096.0
64	105086.5
65	92077.0
66	79219.0
67	66361.0
68	66361.0
69	56509.5
70	46658.0
71	38708.5
72	30759.0
73	24354.5
74	17950.0
75	17950.0
76	13875.0
77	9800.0
78	7676.0
79	5552.0
80	4375.0
81	3198.0
82	3198.0
83	2322.0
84	1446.0
85	1149.0
86	852.0
87	682.0
88	512.0
89	512.0
90	412.5
91	313.0
92	186.0
93	59.0
94	40.5
95	22.0
96	22.0
97	13.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2651708.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.861693977046215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.25073061087835	46.434432160410175
2	11.232217156512686	12.998302235877304
3	3.4951812582981523	6.06711325085865
4	1.5573935249156514	3.604537101620109
5	0.85052721862255	2.460647282154313
6	0.539170666195292	1.8718396853275272
7	0.3819068537131608	1.5468444248101225
8	0.2488773416354378	1.1520371663624387
9	0.18902571122701112	0.9843613071129744
>10	1.1010212171185902	11.788362286739002
>50	0.0989451997770204	3.957433760049825
>100	0.05226309356714223	5.343234101274026
>500	0.0024139394986653993	0.8843668981223028
>1k	1.3048321614205066E-4	0.1436761179071367
>5k	1.9572482421307598E-4	0.7628122213740176
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8006	0.30191861245657514	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6437	0.2427492016466368	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5764	0.21736933327500615	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2739	0.1032919160028178	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.771154289989697E-5
2	0.0	0.0	0.0	0.0	3.771154289989697E-5
3	0.0	0.0	0.0	0.0	3.771154289989697E-5
4	0.0	0.0	0.0	0.0	3.771154289989697E-5
5	0.0	0.0	0.0	0.0	3.771154289989697E-5
6	0.0	0.0	0.0	0.0	7.542308579979395E-5
7	0.0	0.0	0.0	0.0	7.542308579979395E-5
8	0.0	0.0	0.0	0.0	7.542308579979395E-5
9	0.0	0.0	0.0	3.771154289989697E-5	7.542308579979395E-5
10	0.0	0.0	0.0	3.771154289989697E-5	7.542308579979395E-5
11	0.0	0.0	0.0	3.771154289989697E-5	7.542308579979395E-5
12	0.0	0.0	0.0	3.771154289989697E-5	7.542308579979395E-5
13	0.0	0.0	0.0	3.771154289989697E-5	7.542308579979395E-5
14	0.0	0.0	0.0	1.1313462869969092E-4	7.542308579979395E-5
15	0.0	0.0	0.0	1.508461715995879E-4	7.542308579979395E-5
16	0.0	0.0	0.0	1.8855771449948486E-4	7.542308579979395E-5
17	0.0	0.0	0.0	2.639808002992788E-4	7.542308579979395E-5
18	0.0	0.0	0.0	3.7711542899896973E-4	7.542308579979395E-5
19	0.0	0.0	0.0	5.656731434984546E-4	7.542308579979395E-5
20	0.0	0.0	0.0	6.410962292982485E-4	7.542308579979395E-5
21	0.0	0.0	0.0	8.673654866976304E-4	7.542308579979395E-5
22	0.0	0.0	0.0	0.001093634744097012	7.542308579979395E-5
23	0.0	0.0	0.0	0.0016215963446955697	7.542308579979395E-5
24	0.0	0.0	0.0	0.002639808002992788	7.542308579979395E-5
25	0.0	0.0	0.0	0.0032809042322910365	7.542308579979395E-5
26	0.0	0.0	0.0	0.00411055817608877	7.542308579979395E-5
27	0.0	0.0	0.0	0.009616443439473727	7.542308579979395E-5
28	0.0	0.0	0.0	0.032431926893911396	7.542308579979395E-5
29	0.0	0.0	0.0	0.0698040659077093	7.542308579979395E-5
30	0.0	0.0	0.0	0.1169812060754804	7.542308579979395E-5
31	0.0	0.0	0.0	0.3036910549728703	7.542308579979395E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2255	0.0	19.9357	1
TATACGT	75	2.0685508E-4	17.266666	4
TAATACG	135	2.222987E-8	16.444443	4
TAACGCC	240	0.0	15.416667	4
ATAACGC	305	0.0	15.163935	3
TCGTTTA	245	0.0	15.10204	30
CTAGAAC	470	0.0	14.957447	3
CGGCATA	75	0.004106288	14.8	28
GACGGAC	275	0.0	14.8	7
TCTTATA	1905	0.0	14.6640415	37
GTCTAGA	380	0.0	14.118422	1
ACTGATC	385	0.0	13.935064	8
CTTATAC	1165	0.0	13.815452	37
CGTTTAT	255	1.8189894E-12	13.784313	31
TACACCG	150	1.3072258E-6	13.566667	5
CGCATCG	260	1.8189894E-12	13.519231	13
TATACTG	460	0.0	13.271739	5
GTATAGA	360	0.0	12.847222	1
AAGACGG	320	0.0	12.71875	5
GTATCAA	3530	0.0	12.630311	2
>>END_MODULE