FastQCFastQC Report
Fri 10 Feb 2017
ERR1630403.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630403.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448172
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT91280.3728496200430362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT78850.32207704360641326No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60360.24655130440181489No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41200.1682888293796351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG3050.016.98360628
AAGACGG5100.016.6862745
GGTATCA37150.016.2341861
TCTTATA18100.016.14917237
CTAGCGG3100.016.11290429
CGCAAGA5100.015.9607842
CTTATAC10450.015.93301437
ACCGTGC1659.749783E-1015.6969718
CGAACGA2600.015.65384616
GACGGAC5150.015.4466017
ATCTCGC6200.015.21774211
GTACTAG750.00410616714.81
CGTATAC1001.0941658E-414.7999993
TCGCCAT6950.014.64028713
GTAAACG3050.014.55737727
ACGGACC5650.014.407088
CGGACCA5400.014.3888899
TACACGG1702.4403562E-814.14705855
GGACCGT1051.6568374E-414.0952386
GAACCGT1458.9210334E-714.0344846
Produced by FastQC (version 0.11.5)