##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630403.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2448172
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.361837730355546	31.0	31.0	34.0	30.0	34.0
2	31.599235674617635	31.0	31.0	34.0	30.0	34.0
3	31.70928594886307	31.0	31.0	34.0	30.0	34.0
4	35.45472826255671	37.0	35.0	37.0	33.0	37.0
5	35.28675395356208	37.0	35.0	37.0	33.0	37.0
6	35.34993252108104	37.0	35.0	37.0	33.0	37.0
7	35.34254660211783	37.0	35.0	37.0	33.0	37.0
8	35.345068483750325	37.0	35.0	37.0	33.0	37.0
9	37.01577258460598	39.0	37.0	39.0	33.0	39.0
10	36.831147893203585	38.0	37.0	39.0	33.0	39.0
11	36.92813331743031	39.0	37.0	39.0	33.0	39.0
12	36.8350912435891	38.0	37.0	39.0	33.0	39.0
13	36.86272614832618	39.0	37.0	39.0	33.0	39.0
14	37.962282061881275	40.0	37.0	41.0	33.0	41.0
15	37.949415318858314	40.0	37.0	41.0	33.0	41.0
16	37.88871778616862	40.0	37.0	41.0	33.0	41.0
17	37.85840823275489	40.0	37.0	41.0	33.0	41.0
18	37.7956769377315	39.0	37.0	41.0	33.0	41.0
19	37.8330587066595	40.0	37.0	41.0	33.0	41.0
20	37.79983636770619	39.0	37.0	41.0	33.0	41.0
21	37.73470082984365	39.0	37.0	41.0	32.0	41.0
22	37.697277805644376	39.0	37.0	40.0	32.0	41.0
23	37.626234186160126	39.0	37.0	40.0	32.0	41.0
24	37.580227206258385	39.0	37.0	41.0	32.0	41.0
25	37.55915107271875	39.0	37.0	40.0	32.0	41.0
26	37.355840194234716	39.0	36.0	40.0	32.0	41.0
27	37.22470929330129	39.0	36.0	40.0	31.0	41.0
28	37.13496886656657	39.0	36.0	40.0	31.0	41.0
29	37.06074042183311	39.0	36.0	40.0	31.0	41.0
30	36.973378912919515	39.0	36.0	40.0	31.0	41.0
31	36.89855328792258	39.0	36.0	40.0	31.0	41.0
32	36.79081371733685	39.0	36.0	40.0	30.0	41.0
33	36.68536034232889	39.0	35.0	40.0	30.0	41.0
34	36.61637866947257	39.0	35.0	40.0	30.0	41.0
35	36.4640621655668	38.0	35.0	40.0	30.0	41.0
36	36.36032558169933	38.0	35.0	40.0	30.0	41.0
37	36.29141293994049	38.0	35.0	40.0	30.0	41.0
38	36.15272211266202	38.0	35.0	40.0	29.0	41.0
39	36.05807680179334	38.0	35.0	40.0	29.0	41.0
40	35.937237661406144	38.0	35.0	40.0	29.0	41.0
41	35.80860045781097	38.0	35.0	40.0	28.0	41.0
42	35.75794715403983	38.0	35.0	40.0	28.0	41.0
43	34.49186495066523	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	7.0
10	5.0
11	6.0
12	6.0
13	12.0
14	11.0
15	26.0
16	56.0
17	111.0
18	228.0
19	448.0
20	817.0
21	1473.0
22	2615.0
23	4324.0
24	6676.0
25	10325.0
26	14919.0
27	21249.0
28	29420.0
29	39802.0
30	51825.0
31	66509.0
32	85145.0
33	108690.0
34	141125.0
35	188592.0
36	265999.0
37	403958.0
38	637301.0
39	366487.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.23860578423412	20.601371145491413	13.310216765815476	24.849806304459
2	18.359861970482466	22.570513836446132	34.83823848977931	24.231385703292087
3	19.81927740371183	24.26381806507059	30.746124046839846	25.17078048437773
4	15.211267835756637	16.64458216171086	34.78093042482309	33.36321957770941
5	14.202964497592491	37.252243714902384	34.2766766387329	14.268115148772226
6	33.213801971430115	35.94020354778994	16.233091465795706	14.61290301498424
7	27.82525084021874	31.59557416717453	22.05747798765773	18.521697004948997
8	26.18100362229451	34.58772504546249	20.669789540930946	18.561481791312048
9	26.25326161723931	14.87076888388561	20.50395151974616	38.37201797912892
10	16.73338311197089	27.51240517414626	33.410724409886235	22.343487303996614
11	34.5364623073869	22.777321201288146	22.07116166674564	20.615054824579317
12	20.561545512325115	26.58187414936532	29.774501137991937	23.082079200317622
13	29.01920289914271	20.361518716822182	26.211189409894402	24.408088974140707
14	22.39115552338643	21.83118669766667	25.65448832843444	30.123169450512467
15	24.63821986363703	28.57319665448343	23.07047870819534	23.7181047736842
16	24.209859437980665	27.353102641481076	24.702431038342077	23.734606882196186
17	22.92563594387976	27.45432101992834	26.22421954013035	23.395823496061553
18	22.553521566295178	26.043554129366726	27.667051171241237	23.735873133096856
19	23.544301625866158	26.316369928256673	27.769862575015154	22.369465870862015
20	23.86135451267313	25.621606651820215	27.55317028378725	22.963868551719404
21	23.832884290809634	26.43127198579185	26.575502048058713	23.160341675339804
22	23.820221781802914	26.007894870131675	26.775692230774634	23.39619111729078
23	23.037270257155136	26.317227711124875	27.04536282581453	23.600139205905467
24	23.673418370931454	26.55348562110832	26.95582663309604	22.817269374864185
25	23.829779933762822	25.73193386739167	27.11353614043458	23.324750058410928
26	23.401705435729188	26.67606687765402	26.88785755249223	23.03437013412456
27	23.84121703867212	26.0277872633132	26.418364395965643	23.71263130204904
28	22.899248908981885	26.5351454064502	27.354654820004477	23.210950864563436
29	22.946304426322985	26.715443195984594	27.258991606798872	23.079260770893548
30	22.657925995395747	27.460366346808968	27.056554849904334	22.82515280789095
31	23.42976718956021	26.688851927070484	26.88246577446356	22.998915108905745
32	22.66936310030504	26.58146568133285	26.941775332778906	23.8073958855832
33	22.72152446805208	26.343124584383776	27.33541597567491	23.59993497188923
34	23.364248917151244	26.129536650202684	27.08187986791778	23.424334564728294
35	23.58584282476885	26.205348317030015	27.37115692851646	22.83765192968468
36	22.77135756801401	26.55565050168044	27.253640675573447	23.419351254732103
37	23.616273693188226	25.42909566811482	27.22517045370995	23.729460184987
38	22.87723248203149	25.820734817651704	28.01653641982671	23.285496280490094
39	22.979186102937213	25.614581001661644	27.937334468329837	23.4688984270713
40	22.7074323209317	25.34597242350619	28.603750063312543	23.342845192249566
41	21.92235676251505	25.7233560387097	28.953848013946732	23.400439184828517
42	22.77270551252118	25.219960035487706	28.53014412386058	23.47719032813054
43	22.025699174731187	25.10420019508433	28.81586751257673	24.054233117607748
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	631.0
1	567.5
2	504.0
3	974.5
4	1445.0
5	1445.0
6	2068.0
7	2691.0
8	2619.0
9	2547.0
10	3700.0
11	4853.0
12	4853.0
13	8524.0
14	12195.0
15	18184.0
16	24173.0
17	22892.5
18	21612.0
19	21612.0
20	25315.0
21	29018.0
22	30126.5
23	31235.0
24	35519.0
25	39803.0
26	39803.0
27	43970.0
28	48137.0
29	59597.5
30	71058.0
31	77040.5
32	83023.0
33	83023.0
34	90990.0
35	98957.0
36	107270.5
37	115584.0
38	128065.0
39	140546.0
40	140546.0
41	150445.5
42	160345.0
43	154198.0
44	148051.0
45	156299.0
46	164547.0
47	164547.0
48	168052.0
49	171557.0
50	174319.5
51	177082.0
52	183504.5
53	189927.0
54	189927.0
55	171537.0
56	153147.0
57	143799.0
58	134451.0
59	121840.5
60	109230.0
61	109230.0
62	99865.0
63	90500.0
64	79727.0
65	68954.0
66	59352.0
67	49750.0
68	49750.0
69	43043.5
70	36337.0
71	31466.0
72	26595.0
73	21601.0
74	16607.0
75	16607.0
76	12671.5
77	8736.0
78	7401.5
79	6067.0
80	4687.5
81	3308.0
82	3308.0
83	2486.5
84	1665.0
85	1496.0
86	1327.0
87	1197.0
88	1067.0
89	1067.0
90	876.5
91	686.0
92	419.0
93	152.0
94	102.5
95	53.0
96	53.0
97	33.5
98	14.0
99	9.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2448172.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.80307670836497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.34993634460422	37.13821157540388
2	12.075018836944103	11.302960657608935
3	3.532969804725254	4.960615703366798
4	1.5184476056929073	2.84272079067513
5	0.8115311800458739	1.8991078035458488
6	0.4967849202932124	1.3950637639225318
7	0.33222892394574616	1.0884535068519214
8	0.23456430175169016	0.8782664806342738
9	0.17959146847157462	0.7564889947538714
>10	1.1710423422701475	10.575214397422455
>50	0.1337386374741831	4.423151815082378
>100	0.1446948668244474	14.406093842379173
>500	0.015595659992349242	4.9710344131893205
>1k	0.0035922587622826793	2.4174455613272627
>5k	2.6284820211824487E-4	0.9451706938362071
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9128	0.3728496200430362	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7885	0.32207704360641326	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6036	0.24655130440181489	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4120	0.1682888293796351	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.084680324748425E-5	0.0	0.0	0.0
3	0.0	4.084680324748425E-5	0.0	0.0	0.0
4	0.0	4.084680324748425E-5	0.0	0.0	0.0
5	0.0	4.084680324748425E-5	0.0	0.0	0.0
6	0.0	4.084680324748425E-5	0.0	0.0	0.0
7	0.0	4.084680324748425E-5	0.0	4.084680324748425E-5	0.0
8	0.0	4.084680324748425E-5	0.0	4.084680324748425E-5	0.0
9	0.0	4.084680324748425E-5	0.0	4.084680324748425E-5	0.0
10	0.0	4.084680324748425E-5	0.0	4.084680324748425E-5	0.0
11	0.0	4.084680324748425E-5	0.0	4.084680324748425E-5	0.0
12	0.0	4.084680324748425E-5	0.0	1.63387212989937E-4	0.0
13	0.0	4.084680324748425E-5	0.0	1.63387212989937E-4	0.0
14	0.0	4.084680324748425E-5	0.0	1.63387212989937E-4	0.0
15	0.0	4.084680324748425E-5	0.0	3.6762122922735823E-4	0.0
16	0.0	4.084680324748425E-5	0.0	6.53548851959748E-4	0.0
17	0.0	4.084680324748425E-5	0.0	8.169360649496849E-4	0.0
18	0.0	4.084680324748425E-5	0.0	9.803232779396218E-4	0.0
19	0.0	4.084680324748425E-5	0.0	0.0011028636876820746	0.0
20	0.0	4.084680324748425E-5	0.0	0.0012254040974245275	0.0
21	0.0	4.084680324748425E-5	0.0	0.0017155657363943384	0.0
22	0.0	4.084680324748425E-5	0.0	0.002777582620828929	0.0
23	0.0	4.084680324748425E-5	0.0	0.004248067537738361	0.0
24	0.0	4.084680324748425E-5	0.0	0.006617182126092447	0.0
25	0.0	4.084680324748425E-5	0.0	0.007638352207279554	0.0
26	0.0	4.084680324748425E-5	0.0	0.010415934828108483	0.0
27	0.0	4.084680324748425E-5	0.0	0.021893886540651556	0.0
28	0.0	4.084680324748425E-5	0.0	0.051630359304820087	0.0
29	0.0	1.2254040974245272E-4	0.0	0.0923137753393144	0.0
30	0.0	1.2254040974245272E-4	0.0	0.15386990783327315	0.0
31	0.0	1.2254040974245272E-4	0.0	0.34907678055300034	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	305	0.0	16.983606	28
AAGACGG	510	0.0	16.686274	5
GGTATCA	3715	0.0	16.234186	1
TCTTATA	1810	0.0	16.149172	37
CTAGCGG	310	0.0	16.112904	29
CGCAAGA	510	0.0	15.960784	2
CTTATAC	1045	0.0	15.933014	37
ACCGTGC	165	9.749783E-10	15.696971	8
CGAACGA	260	0.0	15.653846	16
GACGGAC	515	0.0	15.446601	7
ATCTCGC	620	0.0	15.217742	11
GTACTAG	75	0.004106167	14.8	1
CGTATAC	100	1.0941658E-4	14.799999	3
TCGCCAT	695	0.0	14.640287	13
GTAAACG	305	0.0	14.557377	27
ACGGACC	565	0.0	14.40708	8
CGGACCA	540	0.0	14.388889	9
TACACGG	170	2.4403562E-8	14.1470585	5
GGACCGT	105	1.6568374E-4	14.095238	6
GAACCGT	145	8.9210334E-7	14.034484	6
>>END_MODULE