FastQCFastQC Report
Fri 10 Feb 2017
ERR1630402.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630402.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2756131
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC154300.5598427650935315No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT78290.284057615548753No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG53220.19309677225066588No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT52020.1887428427748899No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT51370.18638446430884453No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG50830.18442519604474533No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG49420.17930932891070853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49080.17807571555923865No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT45290.16432455496491277No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG44600.16182104551634158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43880.15920868783087597No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG42950.15583439248714956No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT41180.14941234651037993No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG37910.13754788868889034No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT37720.13685851652189246No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37510.13609657886363166No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC37060.13446385531021565No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG35670.1294205536674418No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT35510.12884002973733832No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC34430.12492149320913991No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG34420.12488521046350844No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT33450.12136578413725618No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG32050.11628619974885084No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA29280.10623587920893456No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA28430.1031518458302599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18200.020.2280241
CAGGACA40500.018.8197544
TGCATCA38050.018.7674114
GACAGGC38600.018.691717
CCAGGAC42300.018.5437363
GCATCAG39000.018.16794815
GCTGCAT39250.018.05222912
GGACAGG42000.017.9714286
ACAGGCT40950.017.9352878
CTGCATC40900.017.5048913
TCCAGGA45350.017.2149942
ATACCGT1851.8189894E-1217.0000026
AGGCCAT42050.016.85017825
ATCAAGC42000.016.69404830
GGCCATC42800.016.2955626
CGTCGTA1151.2436612E-616.08695610
AGAGGCC44100.016.02494423
CATCAGA45600.015.94407816
TCTAGCG1751.3278623E-1015.85714228
ATTAGAC2003.6379788E-1215.7253
Produced by FastQC (version 0.11.5)