##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630402.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2756131
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.857138866040838	33.0	31.0	34.0	30.0	34.0
2	32.0763530470794	33.0	31.0	34.0	30.0	34.0
3	32.18931139339894	34.0	31.0	34.0	30.0	34.0
4	35.83251630637295	37.0	35.0	37.0	35.0	37.0
5	35.76186980952647	37.0	35.0	37.0	35.0	37.0
6	35.804346019837226	37.0	35.0	37.0	35.0	37.0
7	35.78223676595924	37.0	35.0	37.0	35.0	37.0
8	35.777644096017205	37.0	35.0	37.0	35.0	37.0
9	37.53429826085915	39.0	37.0	39.0	35.0	39.0
10	37.42043103176155	39.0	37.0	39.0	34.0	39.0
11	37.48848040967574	39.0	37.0	39.0	35.0	39.0
12	37.411304107097955	39.0	37.0	39.0	34.0	39.0
13	37.41310699672839	39.0	37.0	39.0	34.0	39.0
14	38.67030776113327	40.0	38.0	41.0	34.0	41.0
15	38.68143386508116	40.0	38.0	41.0	35.0	41.0
16	38.652866282480765	40.0	38.0	41.0	34.0	41.0
17	38.612567399735354	40.0	38.0	41.0	34.0	41.0
18	38.511849763309506	40.0	38.0	41.0	34.0	41.0
19	38.54585068706821	40.0	38.0	41.0	34.0	41.0
20	38.51860415923626	40.0	38.0	41.0	34.0	41.0
21	38.47062204227593	40.0	38.0	41.0	34.0	41.0
22	38.43017621441071	40.0	38.0	41.0	34.0	41.0
23	38.38155552112726	40.0	38.0	41.0	34.0	41.0
24	38.339612667177285	40.0	38.0	41.0	34.0	41.0
25	38.32077502847289	40.0	38.0	41.0	34.0	41.0
26	38.13485062937865	40.0	38.0	41.0	33.0	41.0
27	38.02699436274981	40.0	38.0	41.0	33.0	41.0
28	37.953767799861474	40.0	37.0	41.0	33.0	41.0
29	37.89156139530378	40.0	37.0	41.0	33.0	41.0
30	37.83488556966269	40.0	37.0	41.0	33.0	41.0
31	37.803802867135126	40.0	37.0	41.0	33.0	41.0
32	37.72710005438783	40.0	37.0	41.0	33.0	41.0
33	37.63481416521929	40.0	37.0	41.0	32.0	41.0
34	37.55281153181761	40.0	37.0	41.0	32.0	41.0
35	37.41395419883888	40.0	37.0	41.0	31.0	41.0
36	37.33572932491235	40.0	36.0	41.0	31.0	41.0
37	37.25710207533677	40.0	36.0	41.0	31.0	41.0
38	37.16551354053926	40.0	36.0	41.0	31.0	41.0
39	37.093270602884985	39.0	36.0	41.0	31.0	41.0
40	36.98029447802009	39.0	36.0	41.0	31.0	41.0
41	36.851745073075264	39.0	36.0	41.0	30.0	41.0
42	36.827278529213594	39.0	35.0	41.0	30.0	41.0
43	35.569339773762565	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	3.0
10	6.0
11	5.0
12	4.0
13	4.0
14	2.0
15	4.0
16	24.0
17	46.0
18	130.0
19	254.0
20	486.0
21	956.0
22	1603.0
23	3010.0
24	4654.0
25	7365.0
26	11309.0
27	16187.0
28	22834.0
29	31368.0
30	42417.0
31	55828.0
32	72193.0
33	93082.0
34	126092.0
35	166552.0
36	227764.0
37	345417.0
38	649588.0
39	876939.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.017585158325204	17.48008349385425	11.629962436473448	27.872368911347102
2	19.1539153980707	19.273539610417647	34.84536838053053	26.727176610981118
3	20.345767309318752	21.95164163096747	28.449808808071896	29.252782251641886
4	15.34585983032011	14.61211386541496	32.965196501907926	37.076829802357
5	15.537650423728044	36.01418074830261	32.913602437619986	15.534566390349369
6	36.70471396316068	33.07553233137322	15.26070422632306	14.959049479143044
7	31.162270588734714	28.91081737406531	20.607402188067258	19.319509849132714
8	28.18124392490778	33.445507488577285	18.923447397819626	19.44980118869531
9	27.89482793089298	13.63940973778097	18.163432725077293	40.30232960624876
10	17.979479204725752	25.96828670335336	31.867389467336636	24.184844624584244
11	36.89160638590836	21.079041598530694	20.60152438327496	21.427827632285982
12	23.290656358496747	23.84266205053388	28.511162930934702	24.355518660034665
13	30.653151102033977	18.728536488287386	24.175483676211325	26.442828733467312
14	23.398452395767837	19.28279171055367	24.636310828476585	32.68244506520191
15	26.339205211943845	26.119730883619102	21.250513854385005	26.290550050052047
16	25.60146814501923	24.89221303341532	22.330360929868718	27.17595789169673
17	24.492304611065293	25.691739616150322	24.721393866982375	25.09456190580201
18	24.598685621256756	23.889720771617895	25.444654118400035	26.066939488725318
19	25.651756030464444	23.738421722334678	24.948777833854777	25.6610444133461
20	25.201886267379887	24.821933355127168	24.821280265705802	25.154900111787136
21	27.114458637851396	23.694011641681765	24.126901079810793	25.06462864065605
22	26.31583912375718	24.426052317542236	24.389007634252508	24.869100924448077
23	25.733138228915823	24.716241717102708	24.100668654719243	25.449951399262225
24	26.04687513039112	24.24086518383923	24.48998251534488	25.22227717042477
25	25.875439157282436	23.9667490405935	24.918808285963184	25.239003516160878
26	26.443808367599363	24.24511026507811	24.82342094769806	24.487660419624465
27	26.653087244401664	24.153641463341184	23.73501114424532	25.45826014801183
28	25.371000144768157	23.403386849173717	25.326082105676402	25.899530900381727
29	25.246260065287174	23.547683328550058	25.718879109882657	25.48717749628011
30	24.9042951877106	24.297103439568	25.12572878429944	25.672872588421956
31	24.99217199763001	24.093266974610422	25.27383495196709	25.64072607579248
32	24.752270483514753	24.049509983378876	25.46265036023324	25.735569172873134
33	25.718770261645762	24.337341004473302	24.7623570868003	25.181531647080636
34	25.96411418760574	24.400545547363315	24.743925452019518	24.89141481301143
35	26.13301036852022	24.02374923398053	25.271585421737935	24.571654975761312
36	25.934362336187938	24.441653898163768	23.69985316372843	25.924130601919863
37	24.984915448503717	23.935981272298015	25.120612917165403	25.958490362032865
38	25.95671250749692	23.903834759668534	25.117020925347887	25.022431807486655
39	24.236765233582876	24.28317086524552	25.657706400748005	25.8223575004236
40	25.201777419142996	22.89521071385939	26.70540696360224	25.197604903395376
41	24.012138755378466	23.31997281696697	26.809828705529597	25.858059722124967
42	24.006043254112377	24.045446315868148	25.81891063958861	26.129599790430863
43	24.243840368981008	22.22521353302873	27.25784804858695	26.27309804940331
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	590.0
1	503.5
2	417.0
3	771.5
4	1126.0
5	1126.0
6	1481.0
7	1836.0
8	1607.0
9	1378.0
10	2006.5
11	2635.0
12	2635.0
13	4646.0
14	6657.0
15	8970.0
16	11283.0
17	11478.5
18	11674.0
19	11674.0
20	14760.5
21	17847.0
22	18746.0
23	19645.0
24	23507.5
25	27370.0
26	27370.0
27	32646.0
28	37922.0
29	44523.5
30	51125.0
31	59174.5
32	67224.0
33	67224.0
34	78213.0
35	89202.0
36	99566.0
37	109930.0
38	117283.0
39	124636.0
40	124636.0
41	133376.0
42	142116.0
43	147947.5
44	153779.0
45	156770.0
46	159761.0
47	159761.0
48	162049.0
49	164337.0
50	175990.5
51	187644.0
52	198737.5
53	209831.0
54	209831.0
55	213354.0
56	216877.0
57	204009.0
58	191141.0
59	172941.5
60	154742.0
61	154742.0
62	150719.0
63	146696.0
64	128315.5
65	109935.0
66	93533.0
67	77131.0
68	77131.0
69	66873.0
70	56615.0
71	52299.0
72	47983.0
73	54204.5
74	60426.0
75	60426.0
76	57817.0
77	55208.0
78	41791.0
79	28374.0
80	17419.5
81	6465.0
82	6465.0
83	4381.0
84	2297.0
85	1716.0
86	1135.0
87	897.5
88	660.0
89	660.0
90	509.0
91	358.0
92	222.0
93	86.0
94	57.0
95	28.0
96	28.0
97	17.5
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2756131.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.09421928957143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.16913693523279	39.51860490597908
2	10.109420696836883	9.724093917684101
3	2.9944785874122597	4.320513295227938
4	1.3431182336592529	2.583848914457197
5	0.7487751146575106	1.800587728145615
6	0.4649007120744926	1.3415422076633132
7	0.32239763745205563	1.0853823871841237
8	0.2431908595104778	0.9356859621213018
9	0.1863239163150296	0.8064992961132109
>10	1.1598995802694825	10.98936579786309
>50	0.14100142053075038	4.755666359337654
>100	0.09811489080971346	8.79232600165112
>500	0.009847968429623502	3.356764045623218
>1k	0.008938925189964558	8.385953301537867
>5k	3.78768016523922E-4	1.0410030832537187
>10k+	7.57536033047844E-5	0.5621627961573822
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	15430	0.5598427650935315	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	7829	0.284057615548753	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	5322	0.19309677225066588	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	5202	0.1887428427748899	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	5137	0.18638446430884453	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	5083	0.18442519604474533	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	4942	0.17930932891070853	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4908	0.17807571555923865	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	4529	0.16432455496491277	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	4460	0.16182104551634158	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4388	0.15920868783087597	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	4295	0.15583439248714956	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	4118	0.14941234651037993	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	3791	0.13754788868889034	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	3772	0.13685851652189246	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3751	0.13609657886363166	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	3706	0.13446385531021565	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	3567	0.1294205536674418	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	3551	0.12884002973733832	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	3443	0.12492149320913991	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	3442	0.12488521046350844	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	3345	0.12136578413725618	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	3205	0.11628619974885084	No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA	2928	0.10623587920893456	No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA	2843	0.1031518458302599	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.451309825258669E-4	0.0	0.0	0.0	0.0
6	1.451309825258669E-4	0.0	0.0	0.0	0.0
7	1.814137281573336E-4	0.0	0.0	0.0	0.0
8	1.814137281573336E-4	0.0	0.0	3.628274563146672E-5	0.0
9	1.814137281573336E-4	0.0	0.0	3.628274563146672E-5	0.0
10	2.902619650517338E-4	0.0	0.0	1.0884823689440016E-4	0.0
11	3.628274563146672E-4	0.0	0.0	1.0884823689440016E-4	0.0
12	3.628274563146672E-4	0.0	0.0	1.451309825258669E-4	3.628274563146672E-5
13	3.628274563146672E-4	0.0	0.0	2.1769647378880032E-4	3.628274563146672E-5
14	3.628274563146672E-4	0.0	0.0	2.1769647378880032E-4	3.628274563146672E-5
15	3.991102019461339E-4	0.0	0.0	2.902619650517338E-4	3.628274563146672E-5
16	3.991102019461339E-4	0.0	0.0	3.991102019461339E-4	3.628274563146672E-5
17	4.3539294757760064E-4	0.0	0.0	6.168066757349343E-4	3.628274563146672E-5
18	4.7167569320906736E-4	0.0	0.0	6.530894213664009E-4	3.628274563146672E-5
19	4.7167569320906736E-4	0.0	0.0	7.982204038922678E-4	3.628274563146672E-5
20	4.7167569320906736E-4	0.0	0.0	0.0011247651145754683	3.628274563146672E-5
21	5.442411844720008E-4	0.0	0.0	0.0013787443339957353	3.628274563146672E-5
22	5.442411844720008E-4	0.0	0.0	0.0017052890446789357	3.628274563146672E-5
23	5.442411844720008E-4	0.0	0.0	0.00232209572041387	3.628274563146672E-5
24	6.530894213664009E-4	0.0	0.0	0.0039911020194613395	3.628274563146672E-5
25	6.893721669978676E-4	0.0	0.0	0.005442411844720008	3.628274563146672E-5
26	6.893721669978676E-4	0.0	0.0	0.008417596986500278	3.628274563146672E-5
27	6.893721669978676E-4	0.0	0.0	0.01890331047399416	3.628274563146672E-5
28	7.256549126293344E-4	0.0	0.0	0.05039673368210727	3.628274563146672E-5
29	7.256549126293344E-4	0.0	0.0	0.095387338265126	3.628274563146672E-5
30	7.256549126293344E-4	0.0	0.0	0.15474591011820557	3.628274563146672E-5
31	7.256549126293344E-4	0.0	0.0	0.31170506771993056	3.628274563146672E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1820	0.0	20.228024	1
CAGGACA	4050	0.0	18.819754	4
TGCATCA	3805	0.0	18.76741	14
GACAGGC	3860	0.0	18.69171	7
CCAGGAC	4230	0.0	18.543736	3
GCATCAG	3900	0.0	18.167948	15
GCTGCAT	3925	0.0	18.052229	12
GGACAGG	4200	0.0	17.971428	6
ACAGGCT	4095	0.0	17.935287	8
CTGCATC	4090	0.0	17.50489	13
TCCAGGA	4535	0.0	17.214994	2
ATACCGT	185	1.8189894E-12	17.000002	6
AGGCCAT	4205	0.0	16.850178	25
ATCAAGC	4200	0.0	16.694048	30
GGCCATC	4280	0.0	16.29556	26
CGTCGTA	115	1.2436612E-6	16.086956	10
AGAGGCC	4410	0.0	16.024944	23
CATCAGA	4560	0.0	15.944078	16
TCTAGCG	175	1.3278623E-10	15.857142	28
ATTAGAC	200	3.6379788E-12	15.725	3
>>END_MODULE