FastQCFastQC Report
Fri 10 Feb 2017
ERR1630401.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630401.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2472019
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC131050.5301334658026495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT65230.26387337637777053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT59960.2425547700078357No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT58950.2384690408932941No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47150.19073477995112498No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG42480.17184333939180887No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG40660.16448093643293193No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT39710.16063792389945222No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT38290.1548936314809878No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG37950.15351823752163718No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG33840.1368921517188986No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT33630.136042643685182No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG33200.1343031748542386No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT29510.11937610511893314No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC29510.11937610511893314No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT29040.11747482523394844No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG28380.11480494284226779No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27120.10970789463996838No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT26850.10861567002518994No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG26160.10582442934297835No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT26020.10525809065383396No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC24810.10036330626908613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGC29100.020.597947
TGCATCA28300.020.59187314
CAGGACA30950.020.4426484
CCAGGAC31800.020.2452853
CGTAATA555.1453954E-420.1818182
TCCAGGA33050.019.6475052
ACAGGCT31200.019.5673088
GCTGCAT30600.019.28594812
GGACAGG33150.018.8627456
GCATCAG31000.018.85806515
CCGTAAT609.2409144E-418.51
CTGCATC32700.018.047413
ATCAAGC32400.017.9861130
CTAGCGG2300.017.69565229
AGGCCAT34300.017.09766825
CATCAAG34200.017.03947429
GGCCATC35250.016.79432726
CATCAGA35500.016.78028316
TCAAGCA34050.016.6255531
AGAGGCC35150.016.57894723
Produced by FastQC (version 0.11.5)