##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630401.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2472019
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.874990847562255	33.0	31.0	34.0	30.0	34.0
2	32.0712089996072	33.0	31.0	34.0	30.0	34.0
3	32.20996238297521	34.0	31.0	34.0	30.0	34.0
4	35.85207840231001	37.0	35.0	37.0	35.0	37.0
5	35.76861100177628	37.0	35.0	37.0	35.0	37.0
6	35.818606976726315	37.0	35.0	37.0	35.0	37.0
7	35.798368054614464	37.0	35.0	37.0	35.0	37.0
8	35.77722460871053	37.0	35.0	37.0	35.0	37.0
9	37.49123166124532	39.0	37.0	39.0	35.0	39.0
10	37.40618700746232	39.0	37.0	39.0	34.0	39.0
11	37.49077333143475	39.0	37.0	39.0	35.0	39.0
12	37.36817799539567	39.0	37.0	39.0	34.0	39.0
13	37.399699597778174	39.0	37.0	39.0	34.0	39.0
14	38.644813005078035	40.0	38.0	41.0	34.0	41.0
15	38.63631104777107	40.0	38.0	41.0	34.0	41.0
16	38.62743732956745	40.0	38.0	41.0	34.0	41.0
17	38.59560788165463	40.0	38.0	41.0	34.0	41.0
18	38.524084564074954	40.0	38.0	41.0	34.0	41.0
19	38.53804157654128	40.0	38.0	41.0	34.0	41.0
20	38.48703145081004	40.0	38.0	41.0	34.0	41.0
21	38.460188615055145	40.0	38.0	41.0	34.0	41.0
22	38.42473702669761	40.0	38.0	41.0	34.0	41.0
23	38.37840485853871	40.0	38.0	41.0	34.0	41.0
24	38.355447510718975	40.0	38.0	41.0	34.0	41.0
25	38.332410066427485	40.0	38.0	41.0	34.0	41.0
26	38.15758657194787	40.0	38.0	41.0	33.0	41.0
27	38.07230041516671	40.0	38.0	41.0	33.0	41.0
28	37.98634395609419	40.0	37.0	41.0	33.0	41.0
29	37.912791123369196	40.0	37.0	41.0	33.0	41.0
30	37.86152816786603	40.0	37.0	41.0	33.0	41.0
31	37.775248086685416	40.0	37.0	41.0	33.0	41.0
32	37.68458778027192	40.0	37.0	41.0	33.0	41.0
33	37.617266695765686	40.0	37.0	41.0	32.0	41.0
34	37.51453852094179	40.0	37.0	41.0	32.0	41.0
35	37.413069640645965	40.0	37.0	41.0	32.0	41.0
36	37.31996315562299	40.0	36.0	41.0	31.0	41.0
37	37.24470887966476	40.0	36.0	41.0	31.0	41.0
38	37.14896608804382	39.0	36.0	41.0	31.0	41.0
39	37.06147040131973	39.0	36.0	41.0	31.0	41.0
40	36.94513108515752	39.0	36.0	41.0	31.0	41.0
41	36.80461881563208	39.0	35.0	41.0	30.0	41.0
42	36.75415358862533	39.0	35.0	41.0	30.0	41.0
43	35.56846245922867	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	5.0
10	8.0
11	5.0
12	6.0
13	3.0
14	2.0
15	12.0
16	16.0
17	62.0
18	93.0
19	197.0
20	426.0
21	805.0
22	1550.0
23	2549.0
24	4166.0
25	6559.0
26	9787.0
27	14204.0
28	20210.0
29	27996.0
30	37630.0
31	49564.0
32	64333.0
33	84129.0
34	113731.0
35	152454.0
36	209127.0
37	317974.0
38	580431.0
39	773982.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.23409326546439	17.648043967299603	11.727053877822136	27.390808889413876
2	18.53590122082395	19.338119973996964	35.05037784903757	27.075600956141514
3	19.870882869427785	22.047241546282613	29.05321520587018	29.028660378419424
4	14.64365767415218	14.607654714628001	33.616893721286125	37.1317938899337
5	14.814368336165701	36.777508587110376	33.52607726720547	14.882045809518454
6	36.4018237723901	33.87627684091425	15.17909045197468	14.542808934720972
7	31.10639521783611	29.503333105449432	20.43956781885576	18.9507038578587
8	28.554594442842063	33.80447318568344	18.62109474077667	19.019837630697822
9	27.665038173250288	13.652726779203558	18.190475073209388	40.491759974336766
10	17.805647933935784	26.39126155583756	31.74421394010321	24.05887657012345
11	37.183977954861994	21.263995139195938	20.438677858058536	21.11334904788353
12	23.14614086704026	24.220930340745763	28.31305099192199	24.31987780029199
13	30.56101106019007	18.427002381454187	24.46850125342888	26.54348530492686
14	23.108802966320244	19.18249819277279	24.797260862477188	32.911437978429774
15	26.060883836248834	26.44105890771875	21.278274964715077	26.21978229131734
16	25.426584504407124	25.200170387039904	22.656379259220905	26.716865849332066
17	24.130882489171807	26.213714376790794	24.888562749719966	24.766840384317433
18	24.51162389933087	24.182985648573087	25.798507212120942	25.506883239975096
19	25.354416774304728	23.995891617337893	25.363275929513485	25.286415678843895
20	24.909153206346716	24.98099731434103	25.169628550589618	24.940220928722635
21	26.94012465114548	24.02772794222051	24.324408509805142	24.707738896828868
22	26.126821840770642	24.48569367792076	24.637755615956024	24.749728865352573
23	25.25834146096774	24.716476693747094	24.697140272789163	25.328041572496
24	25.560240435045202	24.593702556493298	24.82687228536674	25.019184723094767
25	25.437992183717036	24.18945809073474	25.331884585029485	25.04066514051874
26	25.919986860942412	24.740101107637116	25.235930629983024	24.103981401437448
27	26.210073628074866	24.410491990555087	24.192856122869603	25.186578258500443
28	24.883667965335217	23.916644653621187	25.814526506471026	25.385160874572566
29	24.90984090332639	23.956167003570766	26.017316209948227	25.11667588315462
30	24.53075805647125	24.804016473983413	25.4825711291054	25.182654340439942
31	24.805310962415742	24.606768799107126	25.48026532158531	25.107654916891818
32	24.369756057700204	24.4678135564492	25.512101646467926	25.650328739382665
33	25.045034038977853	24.735853567468535	25.071045165914985	25.14806722763862
34	25.64151003693742	24.806443639794033	24.98961375296873	24.562432570299826
35	25.884307523526317	24.507457264689307	25.602149498041882	24.006085713742493
36	25.308988320882648	24.98981601678628	24.14892442169741	25.55227124063367
37	24.782536056559437	24.365913045166725	25.439731652547977	25.411819245725866
38	25.447215413797387	24.364861273315455	25.531478520189367	24.65644479269779
39	24.092897344235624	24.70292501797114	25.711210148465685	25.49296748932755
40	24.935083427756826	23.55710049154153	26.75096752897126	24.756848551730386
41	23.60082992889618	23.865391002253624	26.986402612601278	25.547376456248923
42	23.888044549819398	24.620482285937122	25.965900747526614	25.525572416716862
43	24.031247332645904	22.742948173132973	27.344207305849995	25.881597188371124
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	459.0
1	358.0
2	257.0
3	645.5
4	1034.0
5	1034.0
6	1339.5
7	1645.0
8	1524.0
9	1403.0
10	1960.5
11	2518.0
12	2518.0
13	4757.5
14	6997.0
15	10379.5
16	13762.0
17	12995.0
18	12228.0
19	12228.0
20	14584.5
21	16941.0
22	16095.5
23	15250.0
24	18726.0
25	22202.0
26	22202.0
27	26845.0
28	31488.0
29	37520.5
30	43553.0
31	50739.0
32	57925.0
33	57925.0
34	68338.5
35	78752.0
36	89798.0
37	100844.0
38	109326.0
39	117808.0
40	117808.0
41	128322.5
42	138837.0
43	143837.0
44	148837.0
45	154104.0
46	159371.0
47	159371.0
48	161353.0
49	163335.0
50	169003.5
51	174672.0
52	179530.0
53	184388.0
54	184388.0
55	187900.5
56	191413.0
57	178385.0
58	165357.0
59	151495.5
60	137634.0
61	137634.0
62	130979.5
63	124325.0
64	108272.0
65	92219.0
66	78727.0
67	65235.0
68	65235.0
69	56385.0
70	47535.0
71	43145.0
72	38755.0
73	41960.5
74	45166.0
75	45166.0
76	42499.0
77	39832.0
78	30282.0
79	20732.0
80	12839.0
81	4946.0
82	4946.0
83	3374.5
84	1803.0
85	1469.5
86	1136.0
87	953.5
88	771.0
89	771.0
90	646.5
91	522.0
92	307.5
93	93.0
94	62.0
95	31.0
96	31.0
97	17.0
98	3.0
99	4.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2472019.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.01794169766721
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.01708773318795	35.5718664869211
2	11.75978595963402	10.588027174156995
3	3.6185059274649376	4.886930666258394
4	1.6358427699683358	2.945690977799399
5	0.8814105543030347	1.9839644472661278
6	0.5680775005483362	1.5344207879664906
7	0.36133094559588186	1.1386462809678861
8	0.28743356267191256	1.0351733893053545
9	0.21338086785565058	0.8645370721670977
>10	1.3357814317783192	11.910815648369075
>50	0.16997335666016816	5.32157126315203
>100	0.13216650465967594	11.43355192926261
>500	0.01218351908788417	3.7792499666129165
>1k	0.006678373425951305	5.725002424671041
>5k	2.7074486861964757E-4	0.748122562488149
>10k+	9.024828953988251E-5	0.5324289226353424
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	13105	0.5301334658026495	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6523	0.26387337637777053	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5996	0.2425547700078357	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	5895	0.2384690408932941	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4715	0.19073477995112498	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	4248	0.17184333939180887	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	4066	0.16448093643293193	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	3971	0.16063792389945222	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	3829	0.1548936314809878	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	3795	0.15351823752163718	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	3384	0.1368921517188986	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	3363	0.136042643685182	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	3320	0.1343031748542386	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	2951	0.11937610511893314	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	2951	0.11937610511893314	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	2904	0.11747482523394844	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	2838	0.11480494284226779	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2712	0.10970789463996838	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	2685	0.10861567002518994	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	2616	0.10582442934297835	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	2602	0.10525809065383396	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	2481	0.10036330626908613	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.0452763510312826E-5
2	4.0452763510312826E-5	0.0	0.0	0.0	4.0452763510312826E-5
3	4.0452763510312826E-5	0.0	0.0	0.0	4.0452763510312826E-5
4	4.0452763510312826E-5	0.0	0.0	0.0	4.0452763510312826E-5
5	1.2135829053093847E-4	4.0452763510312826E-5	0.0	0.0	4.0452763510312826E-5
6	1.2135829053093847E-4	4.0452763510312826E-5	0.0	0.0	4.0452763510312826E-5
7	1.2135829053093847E-4	4.0452763510312826E-5	0.0	0.0	4.0452763510312826E-5
8	1.2135829053093847E-4	4.0452763510312826E-5	0.0	0.0	4.0452763510312826E-5
9	1.2135829053093847E-4	4.0452763510312826E-5	0.0	0.0	4.0452763510312826E-5
10	1.2135829053093847E-4	4.0452763510312826E-5	0.0	0.0	8.090552702062565E-5
11	2.831693445721898E-4	4.0452763510312826E-5	0.0	0.0	8.090552702062565E-5
12	2.831693445721898E-4	4.0452763510312826E-5	0.0	4.0452763510312826E-5	8.090552702062565E-5
13	2.831693445721898E-4	4.0452763510312826E-5	0.0	4.0452763510312826E-5	8.090552702062565E-5
14	2.831693445721898E-4	4.0452763510312826E-5	0.0	1.618110540412513E-4	8.090552702062565E-5
15	2.831693445721898E-4	4.0452763510312826E-5	0.0	2.831693445721898E-4	8.090552702062565E-5
16	2.831693445721898E-4	4.0452763510312826E-5	0.0	6.067914526546923E-4	8.090552702062565E-5
17	3.236221080825026E-4	4.0452763510312826E-5	0.0	0.0012944884323300104	1.2135829053093847E-4
18	3.236221080825026E-4	4.0452763510312826E-5	0.0	0.001415846722860949	1.2135829053093847E-4
19	3.236221080825026E-4	8.090552702062565E-5	0.0	0.0016990160674331387	1.2135829053093847E-4
20	3.6407487159281543E-4	8.090552702062565E-5	0.0	0.00214399646604658	1.618110540412513E-4
21	3.6407487159281543E-4	8.090552702062565E-5	0.0	0.0024271658106187694	2.4271658106187694E-4
22	4.0452763510312825E-4	8.090552702062565E-5	0.0	0.0036002959524178415	2.4271658106187694E-4
23	4.0452763510312825E-4	8.090552702062565E-5	0.0	0.005137500965809729	2.4271658106187694E-4
24	4.0452763510312825E-4	8.090552702062565E-5	0.0	0.007362402958876934	2.4271658106187694E-4
25	4.0452763510312825E-4	8.090552702062565E-5	0.0	0.008616438627696631	2.4271658106187694E-4
26	4.0452763510312825E-4	8.090552702062565E-5	0.0	0.010841340620763838	2.831693445721898E-4
27	4.0452763510312825E-4	8.090552702062565E-5	0.0	0.018648723978254213	2.831693445721898E-4
28	4.0452763510312825E-4	1.618110540412513E-4	0.0	0.042556307212849094	2.831693445721898E-4
29	4.0452763510312825E-4	1.618110540412513E-4	0.0	0.07900424713564094	2.831693445721898E-4
30	4.4498039861344107E-4	1.618110540412513E-4	0.0	0.13005563468565573	2.831693445721898E-4
31	4.4498039861344107E-4	1.618110540412513E-4	0.0	0.2747551697620447	2.831693445721898E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACAGGC	2910	0.0	20.59794	7
TGCATCA	2830	0.0	20.591873	14
CAGGACA	3095	0.0	20.442648	4
CCAGGAC	3180	0.0	20.245285	3
CGTAATA	55	5.1453954E-4	20.181818	2
TCCAGGA	3305	0.0	19.647505	2
ACAGGCT	3120	0.0	19.567308	8
GCTGCAT	3060	0.0	19.285948	12
GGACAGG	3315	0.0	18.862745	6
GCATCAG	3100	0.0	18.858065	15
CCGTAAT	60	9.2409144E-4	18.5	1
CTGCATC	3270	0.0	18.0474	13
ATCAAGC	3240	0.0	17.98611	30
CTAGCGG	230	0.0	17.695652	29
AGGCCAT	3430	0.0	17.097668	25
CATCAAG	3420	0.0	17.039474	29
GGCCATC	3525	0.0	16.794327	26
CATCAGA	3550	0.0	16.780283	16
TCAAGCA	3405	0.0	16.62555	31
AGAGGCC	3515	0.0	16.578947	23
>>END_MODULE