FastQCFastQC Report
Fri 10 Feb 2017
ERR1630400.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630400.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2487615
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT59050.2373759605083584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51880.20855317241614962No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40010.16083678543504523No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31300.1258233287707302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.019.1607151
CTAGTAC1005.8833284E-616.653
CTTATAC13200.016.39772637
CGAATTA3100.016.11290415
CGCAATA1201.9376075E-615.41666736
GACGGAC2151.2732926E-1114.6279087
TCTAGCG1304.448684E-614.23076928
TAATACT3650.014.1917814
CCGAATT3650.014.19178114
TATACTT3800.014.1184225
GCGTAAA1051.6568544E-414.09523814
CTATACT3550.014.0704224
AAGACGG2900.014.0344835
GTATAGA2405.456968E-1213.8750011
CTAGCAC4250.013.4941183
TAGCACT4300.013.3372114
CTAGCGG1551.8898354E-613.12903229
AACGTGT1153.579775E-412.8695656
GCGAAAG2451.1459633E-1012.83673418
GTATCAA33250.012.7969922
Produced by FastQC (version 0.11.5)