##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630400.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2487615
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.693922492025493	31.0	31.0	34.0	30.0	34.0
2	31.919471863612337	33.0	31.0	34.0	30.0	34.0
3	32.035471726935235	33.0	31.0	34.0	30.0	34.0
4	35.712763832023846	37.0	35.0	37.0	33.0	37.0
5	35.608652062316715	37.0	35.0	37.0	33.0	37.0
6	35.667631446184394	37.0	35.0	37.0	33.0	37.0
7	35.650771522120586	37.0	35.0	37.0	33.0	37.0
8	35.648890604052475	37.0	35.0	37.0	33.0	37.0
9	37.36988038743937	39.0	37.0	39.0	34.0	39.0
10	37.237913021106564	39.0	37.0	39.0	34.0	39.0
11	37.31712463544399	39.0	37.0	39.0	34.0	39.0
12	37.24624630419096	39.0	37.0	39.0	34.0	39.0
13	37.247765429939925	39.0	37.0	39.0	34.0	39.0
14	38.46465711132953	40.0	38.0	41.0	34.0	41.0
15	38.464822329821935	40.0	38.0	41.0	34.0	41.0
16	38.41125294709993	40.0	38.0	41.0	34.0	41.0
17	38.37574463894131	40.0	38.0	41.0	34.0	41.0
18	38.31645170173037	40.0	38.0	41.0	33.0	41.0
19	38.355928871630056	40.0	38.0	41.0	34.0	41.0
20	38.32327590885245	40.0	38.0	41.0	34.0	41.0
21	38.26301578017499	40.0	38.0	41.0	34.0	41.0
22	38.23509465894039	40.0	38.0	41.0	34.0	41.0
23	38.18805160766437	40.0	38.0	41.0	33.0	41.0
24	38.14355798626395	40.0	38.0	41.0	33.0	41.0
25	38.120821348962764	40.0	38.0	41.0	33.0	41.0
26	37.945041736763926	40.0	37.0	41.0	33.0	41.0
27	37.83474613233961	40.0	37.0	41.0	33.0	41.0
28	37.77469584320725	40.0	37.0	41.0	33.0	41.0
29	37.71703177541541	40.0	37.0	41.0	33.0	41.0
30	37.64603043477387	40.0	37.0	41.0	32.0	41.0
31	37.58890302558876	40.0	37.0	41.0	32.0	41.0
32	37.49465733242483	40.0	37.0	41.0	32.0	41.0
33	37.423359322081595	40.0	37.0	41.0	32.0	41.0
34	37.35768959424991	39.0	36.0	41.0	31.0	41.0
35	37.21804981880235	39.0	36.0	41.0	31.0	41.0
36	37.13879438739516	39.0	36.0	41.0	31.0	41.0
37	37.08091083226303	39.0	36.0	41.0	31.0	41.0
38	36.97697835074961	39.0	36.0	41.0	31.0	41.0
39	36.89658045959684	39.0	36.0	40.0	30.0	41.0
40	36.80528377582544	39.0	36.0	40.0	30.0	41.0
41	36.68442544364783	39.0	35.0	40.0	30.0	41.0
42	36.663894131527584	39.0	35.0	40.0	30.0	41.0
43	35.421311175563744	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	3.0
11	4.0
12	2.0
13	3.0
14	4.0
15	8.0
16	22.0
17	57.0
18	152.0
19	265.0
20	532.0
21	936.0
22	1690.0
23	2943.0
24	4639.0
25	7221.0
26	10679.0
27	15827.0
28	22332.0
29	30903.0
30	41570.0
31	54573.0
32	70168.0
33	89722.0
34	118668.0
35	158399.0
36	223231.0
37	342554.0
38	642152.0
39	648345.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.68313826697459	19.32023243146548	13.032603517827315	25.964025783732612
2	18.46592016851482	21.268564468376336	35.216060363038494	25.04945500007035
3	20.029264978704504	23.068159662970356	29.738122659655936	27.164452698669205
4	15.671798087726598	16.09895421920193	33.61963165521996	34.60961603785152
5	14.88538218333625	36.11117475975985	34.209232537993216	14.794210518910683
6	34.57202983580658	34.320061585092546	15.863990207487896	15.243918371612972
7	29.09859443684011	30.242742546575734	21.555345180021828	19.10331783656233
8	26.182347348765784	34.59944565376877	20.27882128062421	18.93938571684123
9	26.506231872697345	14.955449295811448	19.77074426709921	38.767574564392
10	17.044920536337013	26.975275514900822	33.43258502621989	22.547218922542275
11	34.63570528397682	22.27844742856109	21.545335592525372	21.54051169493672
12	20.810575591480195	25.39299690667567	29.788974580069667	24.007452921774473
13	29.575235717745713	20.19966916102371	25.636844929782143	24.588250191448434
14	23.04701491187342	21.149574994522865	25.12732074698054	30.67608934662317
15	25.155259153848164	27.518767976555857	22.49833676031058	24.8276361092854
16	24.820360063755846	26.776651531687985	24.1856155393821	24.217372865174074
17	23.598587401989455	26.89652538676604	25.746588599924024	23.758298611320484
18	23.290903134126463	25.9317458690352	26.746461972612323	24.030889024226017
19	24.397384643524017	25.54354271058825	26.997224248929196	23.06184839695853
20	24.563567915453156	25.12273804427132	26.758240322557953	23.555453717717572
21	23.928381200467115	25.513513948098883	26.057207405486782	24.500897445947224
22	24.199484244949478	25.47230982286246	25.984044958725526	24.344160973462532
23	23.908844415233066	25.789360491876756	26.212737903574308	24.08905718931587
24	24.085318668684664	25.91864094725269	26.277297732969128	23.718742651093518
25	24.65301101657612	25.24413946691912	26.19476888505657	23.908080631448193
26	24.21029781537738	26.032565328638075	26.088763735545896	23.668373120438655
27	24.653533605481556	25.539482596784467	25.304719580803297	24.502264216930676
28	23.955917615869016	25.71877079049612	26.176478273366254	24.14883332026861
29	23.745877075029696	25.759010136214812	26.457068316439642	24.038044472315853
30	23.75118336237722	26.506633864163064	26.27444359356251	23.467739179897208
31	24.23988438725446	25.67499391987908	26.21414487370433	23.87097681916213
32	23.49921511166318	25.842825356817674	26.471901801524755	24.186057729994392
33	23.739123618405582	25.848252241604914	26.34426951115828	24.06835462883123
34	24.41446928081717	25.272359267812742	26.279870478349743	24.033300973020346
35	24.163666805353724	25.41828216986953	26.798318871690352	23.61973215308639
36	23.775664642639637	25.243697276306825	26.663450734940895	24.317187346112643
37	24.342713804185937	24.50431437340585	26.67924899954374	24.47372282286447
38	23.967695965814645	24.744423875881118	27.20316447681816	24.08471568148608
39	23.663267828823994	24.890306578791332	27.24501178839973	24.20141380398494
40	23.202183617641797	24.634157616833793	27.87292245785622	24.290736307668187
41	22.777479634107365	24.585034259722665	28.2282025152606	24.409283590909364
42	23.204354371556693	24.478184928133974	27.717190964035833	24.6002697362735
43	22.93369351768662	24.386289679070114	28.176305417035998	24.50371138620727
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	984.0
1	822.0
2	660.0
3	1111.5
4	1563.0
5	1563.0
6	1963.0
7	2363.0
8	2142.5
9	1922.0
10	2843.0
11	3764.0
12	3764.0
13	6397.5
14	9031.0
15	12019.0
16	15007.0
17	15064.0
18	15121.0
19	15121.0
20	19153.0
21	23185.0
22	24356.0
23	25527.0
24	29348.0
25	33169.0
26	33169.0
27	37471.0
28	41773.0
29	50508.5
30	59244.0
31	68243.0
32	77242.0
33	77242.0
34	84416.5
35	91591.0
36	100868.0
37	110145.0
38	119087.0
39	128029.0
40	128029.0
41	137563.5
42	147098.0
43	146303.5
44	145509.0
45	155290.0
46	165071.0
47	165071.0
48	167540.0
49	170009.0
50	181943.5
51	193878.0
52	203682.5
53	213487.0
54	213487.0
55	195495.0
56	177503.0
57	167044.0
58	156585.0
59	138148.5
60	119712.0
61	119712.0
62	112215.0
63	104718.0
64	92302.5
65	79887.0
66	68737.0
67	57587.0
68	57587.0
69	49010.5
70	40434.0
71	35419.0
72	30404.0
73	25165.5
74	19927.0
75	19927.0
76	15538.0
77	11149.0
78	9328.0
79	7507.0
80	5453.5
81	3400.0
82	3400.0
83	2529.0
84	1658.0
85	1277.0
86	896.0
87	700.0
88	504.0
89	504.0
90	395.0
91	286.0
92	175.5
93	65.0
94	39.0
95	13.0
96	13.0
97	10.5
98	8.0
99	4.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2487615.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.71176501244243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.33231629089846	38.644171490550036
2	11.361874749347935	11.069139457820794
3	3.647569367935213	5.330386257523298
4	1.6374560837144683	3.190535038723738
5	0.9300831564833901	2.2652996080324805
6	0.5853809961618889	1.7108964916672433
7	0.41250562983849864	1.406571411490175
8	0.2884864104516019	1.1242145788161158
9	0.22399268866442845	0.9819971293254135
>10	1.3242926194783065	12.257289629806053
>50	0.13438321148055846	4.577595607734478
>100	0.10709972829915061	10.621716695305041
>500	0.010836578461610074	3.664618773105119
>1k	0.0035570448385436448	2.708571700063486
>5k	1.6544394597877416E-4	0.44699613003666055
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5905	0.2373759605083584	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5188	0.20855317241614962	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4001	0.16083678543504523	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3130	0.1258233287707302	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	4.019914657211827E-5	0.0
8	0.0	0.0	0.0	4.019914657211827E-5	0.0
9	0.0	0.0	0.0	4.019914657211827E-5	0.0
10	0.0	0.0	0.0	4.019914657211827E-5	0.0
11	0.0	4.019914657211827E-5	0.0	4.019914657211827E-5	0.0
12	0.0	4.019914657211827E-5	0.0	8.039829314423654E-5	0.0
13	0.0	8.039829314423654E-5	0.0	1.2059743971635482E-4	0.0
14	0.0	8.039829314423654E-5	0.0	2.0099573286059137E-4	0.0
15	0.0	8.039829314423654E-5	0.0	2.813940260048279E-4	0.0
16	0.0	8.039829314423654E-5	0.0	4.0199146572118274E-4	0.0
17	0.0	1.2059743971635482E-4	0.0	8.84381224586602E-4	0.0
18	4.019914657211827E-5	1.2059743971635482E-4	0.0	9.647795177308386E-4	0.0
19	4.019914657211827E-5	1.2059743971635482E-4	0.0	0.0012059743971635482	0.0
20	4.019914657211827E-5	1.2059743971635482E-4	0.0	0.0015677667163126127	0.0
21	4.019914657211827E-5	1.2059743971635482E-4	0.0	0.0017285633026010857	0.0
22	4.019914657211827E-5	1.2059743971635482E-4	0.0	0.0024119487943270964	0.0
23	4.019914657211827E-5	1.2059743971635482E-4	0.0	0.003376728312057935	0.0
24	4.019914657211827E-5	1.2059743971635482E-4	0.0	0.005547482226952322	0.0
25	4.019914657211827E-5	1.2059743971635482E-4	0.0	0.007155448089837053	0.0
26	4.019914657211827E-5	1.2059743971635482E-4	0.0	0.010974367014188288	0.0
27	4.019914657211827E-5	1.2059743971635482E-4	0.0	0.0270540256430356	0.0
28	4.019914657211827E-5	1.2059743971635482E-4	0.0	0.06604719781799033	0.0
29	4.019914657211827E-5	1.607965862884731E-4	0.0	0.11782369860287865	0.0
30	4.019914657211827E-5	1.607965862884731E-4	0.0	0.19034295901898002	0.0
31	4.019914657211827E-5	1.607965862884731E-4	0.0	0.4029562452389136	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2240	0.0	19.160715	1
CTAGTAC	100	5.8833284E-6	16.65	3
CTTATAC	1320	0.0	16.397726	37
CGAATTA	310	0.0	16.112904	15
CGCAATA	120	1.9376075E-6	15.416667	36
GACGGAC	215	1.2732926E-11	14.627908	7
TCTAGCG	130	4.448684E-6	14.230769	28
TAATACT	365	0.0	14.191781	4
CCGAATT	365	0.0	14.191781	14
TATACTT	380	0.0	14.118422	5
GCGTAAA	105	1.6568544E-4	14.095238	14
CTATACT	355	0.0	14.070422	4
AAGACGG	290	0.0	14.034483	5
GTATAGA	240	5.456968E-12	13.875001	1
CTAGCAC	425	0.0	13.494118	3
TAGCACT	430	0.0	13.337211	4
CTAGCGG	155	1.8898354E-6	13.129032	29
AACGTGT	115	3.579775E-4	12.869565	6
GCGAAAG	245	1.1459633E-10	12.836734	18
GTATCAA	3325	0.0	12.796992	2
>>END_MODULE