FastQCFastQC Report
Fri 10 Feb 2017
ERR1630399.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630399.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523429
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64720.42483108828832844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT59370.3897129436291419No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47180.30969608691970546No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21900.14375464823106293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACT400.001931473823.12500213
TTCGGCG450.003826371220.55555513
GGTATCA23150.020.5377961
CGGTTAT555.1441515E-420.18181824
CCGAATT1202.382876E-1020.04166814
CGAATTA1254.129106E-1019.2415
GGTCCGC500.007035908818.49999827
AGACGTA500.007035908818.4999985
CGACCAT1552.1827873E-1117.90322510
TCGTTAC752.0678232E-417.2666661
TCTATAC1401.869921E-917.1785723
TTCGTTC803.3834687E-416.187529
TATACGA803.3834687E-416.187536
CCGACCA1951.8189894E-1216.1282069
CTCGGCG855.3655735E-415.23529417
TACACGG855.3655735E-415.2352945
AGTTCCG2109.094947E-1214.976195
ATGCGTT1752.239176E-914.836
TCTGTCG2500.014.7999998
GTTCCGA2151.2732926E-1114.6279076
Produced by FastQC (version 0.11.5)