##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630399.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1523429
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.835922776840928	33.0	31.0	34.0	30.0	34.0
2	32.05271135051256	33.0	31.0	34.0	30.0	34.0
3	32.1830259237549	34.0	31.0	34.0	30.0	34.0
4	35.817808378335975	37.0	35.0	37.0	35.0	37.0
5	35.75336034695414	37.0	35.0	37.0	35.0	37.0
6	35.814062880514946	37.0	35.0	37.0	35.0	37.0
7	35.793995650601374	37.0	35.0	37.0	35.0	37.0
8	35.79756129100864	37.0	35.0	37.0	35.0	37.0
9	37.53631117695672	39.0	37.0	39.0	35.0	39.0
10	37.42396462191543	39.0	37.0	39.0	34.0	39.0
11	37.509542617345474	39.0	37.0	39.0	35.0	39.0
12	37.43119961612914	39.0	37.0	39.0	34.0	39.0
13	37.44847249199011	39.0	37.0	39.0	34.0	39.0
14	38.65539188239163	40.0	38.0	41.0	34.0	41.0
15	38.66841382171404	40.0	38.0	41.0	34.0	41.0
16	38.62465726988261	40.0	38.0	41.0	34.0	41.0
17	38.608002079519295	40.0	38.0	41.0	34.0	41.0
18	38.58868775637066	40.0	38.0	41.0	34.0	41.0
19	38.629443840178965	40.0	38.0	41.0	34.0	41.0
20	38.62284688029439	40.0	38.0	41.0	34.0	41.0
21	38.56385889988966	40.0	38.0	41.0	34.0	41.0
22	38.52102592244207	40.0	38.0	41.0	34.0	41.0
23	38.45509570843144	40.0	38.0	41.0	34.0	41.0
24	38.433042826413306	40.0	38.0	41.0	34.0	41.0
25	38.413204028543504	40.0	38.0	41.0	34.0	41.0
26	38.267483420625446	40.0	38.0	41.0	34.0	41.0
27	38.14067147205416	40.0	38.0	41.0	33.0	41.0
28	38.08777173074689	40.0	38.0	41.0	33.0	41.0
29	38.02226556012784	40.0	38.0	41.0	33.0	41.0
30	37.957591065944	40.0	37.0	41.0	33.0	41.0
31	37.88270211476872	40.0	37.0	41.0	33.0	41.0
32	37.78205679424509	40.0	37.0	41.0	33.0	41.0
33	37.69219044668311	40.0	37.0	41.0	33.0	41.0
34	37.63872618940561	40.0	37.0	41.0	33.0	41.0
35	37.51815082947745	40.0	37.0	41.0	32.0	41.0
36	37.424924299064806	40.0	37.0	41.0	32.0	41.0
37	37.37450645878476	40.0	37.0	41.0	32.0	41.0
38	37.264809190319994	40.0	36.0	41.0	31.0	41.0
39	37.17793280815844	39.0	36.0	41.0	31.0	41.0
40	37.08108221649975	39.0	36.0	41.0	31.0	41.0
41	36.966744758042545	39.0	36.0	41.0	31.0	41.0
42	36.92117519096722	39.0	36.0	41.0	31.0	41.0
43	35.739632762668954	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	0.0
12	2.0
13	0.0
14	3.0
15	1.0
16	3.0
17	18.0
18	31.0
19	85.0
20	183.0
21	368.0
22	754.0
23	1282.0
24	2195.0
25	3570.0
26	5557.0
27	8188.0
28	12018.0
29	16722.0
30	22549.0
31	29685.0
32	38454.0
33	50702.0
34	68116.0
35	90551.0
36	127201.0
37	195762.0
38	358922.0
39	490501.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.21063141111269	20.47020241836016	12.420598531339499	24.89856763918765
2	18.760703649464464	22.078876009318453	34.564984649760504	24.595435691456576
3	19.64226754249788	23.821458039724856	30.829595603077003	25.706678814700258
4	14.11782235995245	15.935498142676815	36.80158379550343	33.1450957018673
5	13.82965664957146	37.53657046045467	34.71018340861307	13.923589481360798
6	32.57631304117225	37.01196445649912	16.10321189894639	14.308510603382238
7	27.866214966368634	31.99322055704598	21.655817238611057	18.484747237974332
8	27.332813015900314	32.87202751162017	20.735524924364707	19.05963454811481
9	26.87837765987125	13.686689698043034	20.966254416845157	38.46867822524056
10	16.680462299194776	27.033028779155444	34.149934128863244	22.136574792786536
11	34.357426568615935	22.365138119334738	21.818345324921605	21.459089987127722
12	20.898315576242805	27.244065854069998	29.727279709129867	22.130338860557337
13	30.32120302291738	20.424319085431616	24.913993366280938	24.34048452537007
14	22.381614108698205	22.0671262001708	26.288786677948234	29.26247301318276
15	25.27364255242614	28.357672067421586	23.296392546026105	23.07229283412617
16	23.866159827599446	27.297169740106035	24.704597326163544	24.13207310613097
17	21.806201667422638	26.760879568394717	26.951830377392056	24.48108838679059
18	22.104279227978463	25.708122925321756	28.227242621743446	23.96035522495633
19	22.457626840502577	26.05864795799476	28.444975118630406	23.03875008287226
20	24.267950787335675	25.599748987317426	27.676051854073936	22.456248371272963
21	24.498811562599897	26.085035797533063	26.546297858318308	22.869854781548728
22	24.059736292272234	25.97226388627235	27.412239100082775	22.55576072137264
23	23.777609589944788	25.94535091559895	26.98320696271372	23.293832531742538
24	23.334530194712062	26.181594284997857	27.288308152201385	23.1955673680887
25	23.612193282391235	26.10485949788274	27.086854720502235	23.19609249922379
26	22.96352504777052	26.3048031775685	26.982025417659766	23.74964635700121
27	23.068616916180538	26.149233078797895	27.542996752720345	23.23915325230122
28	23.04262292499355	26.237783316452557	27.52317305237067	23.196420706183222
29	22.370651996253187	26.509341754686304	28.1444031851829	22.97560306387761
30	23.027394122075922	27.23796120462457	27.195097375722792	22.539547297576718
31	23.48274845759139	27.387886143692945	26.77466426069085	22.35470113802481
32	22.546242719549124	26.87181352068262	27.226211395476913	23.355732364291345
33	22.305929583853267	26.60504690405657	27.891815109204305	23.197208402885856
34	22.90241291192435	25.50942643208184	28.265445911821292	23.322714744172522
35	22.590944507423714	26.163477260837233	28.294918896778253	22.9506593349608
36	22.639519137419597	27.238420694367772	27.217874938707347	22.90418522950528
37	23.405488539341185	26.173586035187725	27.536563896315485	22.88436152915561
38	22.672996247281628	26.893475180005105	27.535776199612847	22.89775237310042
39	22.4479119145034	26.474683099770317	28.220022068635952	22.857382917090327
40	21.89560524317182	25.765624784614182	29.091542828710757	23.247227143503242
41	22.066075937900617	25.768906854208502	28.802917628586567	23.362099579304317
42	21.724149927564724	25.792865962247006	29.19545315206682	23.28753095812145
43	21.554926419281763	24.989546608342103	29.220528163767394	24.234998808608736
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	223.0
1	216.0
2	209.0
3	455.5
4	702.0
5	702.0
6	1008.0
7	1314.0
8	1241.5
9	1169.0
10	1636.5
11	2104.0
12	2104.0
13	3986.5
14	5869.0
15	9427.5
16	12986.0
17	12583.0
18	12180.0
19	12180.0
20	15152.5
21	18125.0
22	18320.0
23	18515.0
24	22727.0
25	26939.0
26	26939.0
27	30485.0
28	34031.0
29	39909.0
30	45787.0
31	51008.0
32	56229.0
33	56229.0
34	62045.5
35	67862.0
36	74590.0
37	81318.0
38	83490.5
39	85663.0
40	85663.0
41	90747.0
42	95831.0
43	97222.0
44	98613.0
45	97695.5
46	96778.0
47	96778.0
48	97910.5
49	99043.0
50	97902.0
51	96761.0
52	99785.5
53	102810.0
54	102810.0
55	102723.0
56	102636.0
57	99727.0
58	96818.0
59	89177.0
60	81536.0
61	81536.0
62	72363.5
63	63191.0
64	54728.5
65	46266.0
66	37980.0
67	29694.0
68	29694.0
69	23922.0
70	18150.0
71	14723.0
72	11296.0
73	8656.0
74	6016.0
75	6016.0
76	4441.0
77	2866.0
78	2262.5
79	1659.0
80	1326.5
81	994.0
82	994.0
83	740.5
84	487.0
85	399.5
86	312.0
87	283.0
88	254.0
89	254.0
90	198.0
91	142.0
92	89.0
93	36.0
94	24.5
95	13.0
96	13.0
97	7.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1523429.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.417328649520876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.08712054886573	46.99145433596231
2	13.210193294460886	15.698287930013588
3	3.7490175720604952	6.682698275758418
4	1.4365447533967945	3.4142260692928903
5	0.6549230526850328	1.9456889130767034
6	0.35296950939198585	1.258350320568226
7	0.2292007528345315	0.9532947520520872
8	0.15035058241493016	0.7146743974395823
9	0.12074786708554994	0.6457064132115706
>10	0.8564751458863972	10.136387641612236
>50	0.0968588321758326	4.009267993581389
>100	0.052392731272784245	5.308387661980953
>500	0.002763239197475021	0.9729561116281825
>1k	2.210591357980017E-4	0.45367403436567566
>5k	2.210591357980017E-4	0.8149451494562348
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6472	0.42483108828832844	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5937	0.3897129436291419	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4718	0.30969608691970546	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2190	0.14375464823106293	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.564139188633011E-5	0.0
8	0.0	0.0	0.0	6.564139188633011E-5	0.0
9	0.0	0.0	0.0	6.564139188633011E-5	0.0
10	0.0	0.0	0.0	6.564139188633011E-5	0.0
11	0.0	0.0	0.0	6.564139188633011E-5	0.0
12	0.0	0.0	0.0	6.564139188633011E-5	0.0
13	0.0	0.0	0.0	6.564139188633011E-5	0.0
14	0.0	0.0	0.0	6.564139188633011E-5	0.0
15	0.0	0.0	0.0	6.564139188633011E-5	0.0
16	0.0	0.0	0.0	1.9692417565899035E-4	0.0
17	0.0	0.0	0.0	5.251311350906409E-4	0.0
18	0.0	0.0	0.0	7.220553107496313E-4	0.0
19	0.0	0.0	0.0	7.876967026359614E-4	0.0
20	0.0	0.0	0.0	0.0010502622701812818	0.0
21	0.0	0.0	0.0	0.0013784692296129324	0.0
22	0.0	0.0	0.0	0.002231807324135224	0.0
23	0.0	0.0	0.0	0.003807200729407147	0.0
24	0.0	0.0	0.0	0.00590772526976971	0.0
25	0.0	0.0	0.0	0.006498497796746681	0.0
26	0.0	0.0	0.0	0.007680042850700623	0.0
27	0.0	0.0	0.0	0.012931354201607032	0.0
28	0.0	0.0	0.0	0.02737246041659966	0.0
29	0.0	0.0	0.0	0.05185669959020079	0.0
30	0.0	0.0	0.0	0.088484596262773	0.0
31	0.0	0.0	0.0	0.2053262738204406	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTACT	40	0.0019314738	23.125002	13
TTCGGCG	45	0.0038263712	20.555555	13
GGTATCA	2315	0.0	20.537796	1
CGGTTAT	55	5.1441515E-4	20.181818	24
CCGAATT	120	2.382876E-10	20.041668	14
CGAATTA	125	4.129106E-10	19.24	15
GGTCCGC	50	0.0070359088	18.499998	27
AGACGTA	50	0.0070359088	18.499998	5
CGACCAT	155	2.1827873E-11	17.903225	10
TCGTTAC	75	2.0678232E-4	17.266666	1
TCTATAC	140	1.869921E-9	17.178572	3
TTCGTTC	80	3.3834687E-4	16.1875	29
TATACGA	80	3.3834687E-4	16.1875	36
CCGACCA	195	1.8189894E-12	16.128206	9
CTCGGCG	85	5.3655735E-4	15.235294	17
TACACGG	85	5.3655735E-4	15.235294	5
AGTTCCG	210	9.094947E-12	14.97619	5
ATGCGTT	175	2.239176E-9	14.8	36
TCTGTCG	250	0.0	14.799999	8
GTTCCGA	215	1.2732926E-11	14.627907	6
>>END_MODULE