FastQCFastQC Report
Fri 10 Feb 2017
ERR1630398.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630398.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978383
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT62990.6438174007520572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41570.4248847332792986No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38900.39759480694165783No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17910.18305714633226458No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT10940.11181715136097008No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG303.5990903E-430.8333345
CTAGTAC450.003825407720.5555553
CTAATCG555.142343E-420.18181826
CTTATAC6100.018.8032837
GTTCTAG1002.8738395E-718.51
ATTAGAC609.2354754E-418.53
GGACTAT701.2189623E-418.56
CATCTAG902.1515498E-618.51
CGCGAAA500.00703415218.531
GACGGAC2700.018.57
AATCGTC609.2354754E-418.528
AAGACGG2750.018.1636375
CGGTCCA1900.017.52631610
TCTTATA10950.017.40182737
GCGCAAG3350.017.1194041
CTAGCGG1757.2759576E-1216.91428629
CGGTGAT1005.8759906E-616.65000214
TACCGCA2850.016.2280728
GCGAAAG3200.016.187518
CAGTCGG1151.2416549E-616.08695610
Produced by FastQC (version 0.11.5)