FastQCFastQC Report
Fri 10 Feb 2017
ERR1630397.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630397.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088341
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27340.2512080313063645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26730.2456031703298874No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18260.1677782974270013No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12550.11531312336850306No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTT450.003825678720.55555729
GACGGAC2000.020.357
ATACCGA555.1428523E-420.1818186
ACGGACC2150.019.7906978
CGGACCA2400.019.2708349
TATACCG500.007034647318.55
ACGAACG953.606032E-617.52631615
TATACTG1603.45608E-1117.343755
GTACACT1005.877211E-616.6500024
TAGACTG1352.2188033E-816.4444455
GTCTTAC904.445606E-516.4444451
CAAGACG2950.016.3050844
CGCAATA1151.2419696E-616.08695636
AGACGGA2950.015.6779666
CTATACA957.0591246E-515.5789484
GCGCAAG2750.015.4727281
CGAACGA1001.0932787E-414.816
GGTATAC750.0041042214.81
AAGACGG3200.014.4531265
CGAAAGC2850.014.28070219
Produced by FastQC (version 0.11.5)