##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630397.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1088341
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.8965526429676	31.0	31.0	33.0	28.0	34.0
2	31.14308107477344	31.0	31.0	34.0	28.0	34.0
3	31.25616511736671	31.0	31.0	34.0	28.0	34.0
4	35.0819687947068	37.0	35.0	37.0	32.0	37.0
5	34.65656352191087	35.0	35.0	37.0	31.0	37.0
6	34.742740556498376	35.0	35.0	37.0	32.0	37.0
7	34.74873683891354	35.0	35.0	37.0	32.0	37.0
8	34.73844870311787	35.0	35.0	37.0	32.0	37.0
9	36.36899740063087	38.0	35.0	39.0	32.0	39.0
10	36.04831665810624	37.0	35.0	39.0	32.0	39.0
11	36.21710750582768	37.0	35.0	39.0	32.0	39.0
12	36.073787535340486	37.0	35.0	39.0	32.0	39.0
13	36.15326630164627	37.0	35.0	39.0	32.0	39.0
14	37.11717007812808	38.0	36.0	40.0	32.0	41.0
15	37.06767823687613	38.0	36.0	40.0	32.0	41.0
16	37.00122296228847	38.0	36.0	40.0	31.0	41.0
17	36.93840717201686	38.0	36.0	40.0	31.0	41.0
18	36.8495903397924	38.0	36.0	40.0	31.0	41.0
19	36.87660852618802	38.0	36.0	40.0	31.0	41.0
20	36.83252491636353	38.0	36.0	40.0	31.0	41.0
21	36.75739221438869	38.0	36.0	40.0	31.0	41.0
22	36.6959252660701	38.0	36.0	40.0	31.0	41.0
23	36.610233373547445	38.0	35.0	40.0	30.0	41.0
24	36.553752913838586	38.0	35.0	40.0	30.0	41.0
25	36.536106790059364	38.0	35.0	40.0	30.0	41.0
26	36.277552715555146	38.0	35.0	40.0	30.0	41.0
27	36.14291384777381	38.0	35.0	40.0	30.0	41.0
28	36.006595359358876	38.0	35.0	40.0	29.0	41.0
29	35.914566298614126	38.0	34.0	40.0	29.0	41.0
30	35.81112445455974	38.0	34.0	40.0	29.0	41.0
31	35.735221773322884	38.0	34.0	40.0	28.0	41.0
32	35.62314844336472	38.0	34.0	40.0	28.0	41.0
33	35.48533685673883	38.0	34.0	40.0	27.0	41.0
34	35.40829023256498	38.0	34.0	40.0	27.0	41.0
35	35.249145258701084	38.0	34.0	40.0	27.0	41.0
36	35.1149860200066	38.0	33.0	40.0	26.0	41.0
37	35.031295338501444	38.0	33.0	40.0	26.0	41.0
38	34.89145497596801	38.0	33.0	40.0	26.0	41.0
39	34.786481442856605	38.0	33.0	40.0	25.0	41.0
40	34.64364293911559	37.0	33.0	40.0	25.0	41.0
41	34.514067741636126	37.0	33.0	40.0	24.0	41.0
42	34.43715251010483	37.0	33.0	40.0	24.0	40.0
43	33.097966538061144	36.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	2.0
11	4.0
12	2.0
13	9.0
14	14.0
15	30.0
16	50.0
17	90.0
18	169.0
19	329.0
20	657.0
21	1166.0
22	1946.0
23	3129.0
24	4826.0
25	7284.0
26	10176.0
27	14431.0
28	18993.0
29	25260.0
30	32090.0
31	40705.0
32	51828.0
33	66369.0
34	86110.0
35	114912.0
36	156933.0
37	199326.0
38	190735.0
39	60761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.54148378127811	20.254221792618306	12.75767429509685	25.446620131006735
2	19.759891431086395	21.820734494060225	33.02062496956377	25.39874910528961
3	20.716852530594732	23.526449890245797	29.249104830195684	26.507592748963788
4	15.875171476586841	16.420772533608492	33.555200070566116	34.14885591923855
5	15.221332284642406	36.272179399655066	33.5979256501409	14.90856266556162
6	35.11886440003639	34.946767603168496	15.297778913042878	14.636589083752243
7	29.343192988227035	30.437978537976605	21.302514561153167	18.916313912643187
8	27.367709201435947	33.67317779997262	19.496187316291493	19.462925682299943
9	26.598832535023494	14.172488218306578	19.599739419906076	39.628939826763855
10	17.27895944377727	26.561160518624217	32.88178980668743	23.278090230911083
11	36.57897662589207	21.74842259916699	21.052776657316045	20.619824117624898
12	21.443554915233367	25.30282328792171	29.308553109733072	23.94506868711185
13	30.47675314997781	19.7194629256823	24.51492684737596	25.28885707696393
14	22.861033444481098	20.512045397536248	24.80987117089221	31.817049987090446
15	25.42300620853207	27.998118236839375	21.642573421381716	24.93630213324684
16	25.256146740773346	26.064257434021137	23.839770807127543	24.839825018077974
17	23.929632348684834	26.40211110304583	25.223528287549584	24.444728260719756
18	24.15152971357323	25.200190013975398	26.165696229398687	24.482584043052682
19	24.597253985653396	25.421444198095998	25.98900528418942	23.992296532061182
20	24.78827867368775	25.003009167163597	25.86918989544637	24.339522263702275
21	25.126407991612922	25.16757156075164	25.628364639391517	24.07765580824392
22	25.023039653931995	25.105458675176255	25.64545487122143	24.22604679967032
23	24.38151277954244	25.120711247669618	25.917244687097153	24.58053128569079
24	24.48240027711903	25.742023869357123	25.52150474897114	24.25407110455271
25	24.961386183190744	25.06052790439761	25.644995456387292	24.333090456024355
26	24.642736054233	25.667690549193683	25.625883799287173	24.063689597286146
27	24.88493955479027	25.118506056465755	25.428059771707577	24.56849461703639
28	24.47385516120407	25.37724849105198	25.98808645452115	24.160809893222805
29	24.216490971120265	25.55164236209056	26.059755168646593	24.172111498142588
30	24.11505217574271	25.76554590886496	25.844381494402953	24.275020420989378
31	24.647330202574377	25.511121973719636	25.584444581247972	24.257103242458015
32	24.15685892564922	25.417309464588765	25.563495264811305	24.86233634495071
33	23.96776377991824	25.2767285253427	26.134088488810033	24.621419205929023
34	24.804725724749872	24.973422851845147	25.679543451914427	24.542307971490555
35	24.561235862657014	25.20542734308457	26.213383489182156	24.019953305076257
36	24.245985403471888	25.388366330038103	25.8849937657407	24.480654500749306
37	24.362768654309633	25.0554743412221	26.063706136220173	24.518050868248096
38	23.951684260723432	24.960007938688335	26.689429140315397	24.39887866027284
39	24.045680535787955	24.748217700150963	26.544529701628445	24.661572062432636
40	23.980535512307263	24.874832428439248	26.923271290891364	24.22136076836212
41	23.360509252155346	24.841938326315006	27.246974983024625	24.550577438505027
42	23.708745696431542	24.95752709858399	26.939442693053007	24.394284511931463
43	23.54942063195267	24.40540235091759	27.227679559990847	24.81749745713889
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	127.0
1	128.5
2	130.0
3	249.0
4	368.0
5	368.0
6	577.5
7	787.0
8	809.0
9	831.0
10	1078.0
11	1325.0
12	1325.0
13	2539.0
14	3753.0
15	5386.0
16	7019.0
17	6783.5
18	6548.0
19	6548.0
20	7654.0
21	8760.0
22	8367.0
23	7974.0
24	9610.5
25	11247.0
26	11247.0
27	13835.5
28	16424.0
29	19234.5
30	22045.0
31	25446.0
32	28847.0
33	28847.0
34	33147.5
35	37448.0
36	42099.0
37	46750.0
38	51894.0
39	57038.0
40	57038.0
41	61387.0
42	65736.0
43	68239.0
44	70742.0
45	72988.0
46	75234.0
47	75234.0
48	77890.5
49	80547.0
50	81042.0
51	81537.0
52	80168.5
53	78800.0
54	78800.0
55	76494.5
56	74189.0
57	71186.0
58	68183.0
59	64385.0
60	60587.0
61	60587.0
62	55395.0
63	50203.0
64	44331.0
65	38459.0
66	33477.0
67	28495.0
68	28495.0
69	24576.5
70	20658.0
71	17745.5
72	14833.0
73	12110.5
74	9388.0
75	9388.0
76	7427.5
77	5467.0
78	4327.0
79	3187.0
80	2577.0
81	1967.0
82	1967.0
83	1494.5
84	1022.0
85	840.0
86	658.0
87	597.0
88	536.0
89	536.0
90	454.0
91	372.0
92	224.0
93	76.0
94	54.5
95	33.0
96	33.0
97	19.5
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1088341.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.49478463758135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.52758187530205	53.035658224702466
2	9.620669814315816	12.21724715858525
3	2.7899705353631	5.314457348642327
4	1.2075718761740102	3.066980648482753
5	0.6907312567880458	2.192891619610143
6	0.43343210478609395	1.6512406889043973
7	0.2943167515859331	1.3081305130026946
8	0.21978277256335127	1.1164047848768408
9	0.1701288778133942	0.972206681165537
>10	0.9444080068089383	11.154041143049428
>50	0.06126673547907595	2.6978243391655417
>100	0.03912136576490443	4.318007912836832
>500	4.3629739506641165E-4	0.1711097824404007
>1k	5.817298600885488E-4	0.7837991545354136
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2734	0.2512080313063645	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2673	0.2456031703298874	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1826	0.1677782974270013	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1255	0.11531312336850306	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	9.188296682749248E-5	0.0
15	0.0	0.0	0.0	9.188296682749248E-5	0.0
16	0.0	0.0	0.0	6.431807677924474E-4	0.0
17	0.0	0.0	0.0	0.0011025956019299098	0.0
18	0.0	9.188296682749248E-5	0.0	0.0013782445024123873	0.0
19	0.0	9.188296682749248E-5	0.0	0.0014701274692398797	0.0
20	0.0	9.188296682749248E-5	0.0	0.0015620104360673723	0.0
21	0.0	9.188296682749248E-5	0.0	0.0018376593365498498	0.0
22	0.0	9.188296682749248E-5	0.0	0.002664606037997282	0.0
23	0.0	2.7564890048247746E-4	0.0	0.0036753186730996996	0.0
24	0.0	3.675318673099699E-4	0.0	0.005329212075994564	0.0
25	0.0	3.675318673099699E-4	0.0	0.006431807677924474	0.0
26	0.0	3.675318673099699E-4	0.0	0.009004530749094264	0.0
27	0.0	3.675318673099699E-4	0.0	0.019662954901083392	0.0
28	0.0	3.675318673099699E-4	0.0	0.04180674990650908	0.0
29	0.0	4.5941483413746245E-4	0.0	0.07304695862785653	0.0
30	0.0	4.5941483413746245E-4	0.0	0.11908032500843026	0.0
31	0.0	4.5941483413746245E-4	0.0	0.2568128922828415	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGTTT	45	0.0038256787	20.555557	29
GACGGAC	200	0.0	20.35	7
ATACCGA	55	5.1428523E-4	20.181818	6
ACGGACC	215	0.0	19.790697	8
CGGACCA	240	0.0	19.270834	9
TATACCG	50	0.0070346473	18.5	5
ACGAACG	95	3.606032E-6	17.526316	15
TATACTG	160	3.45608E-11	17.34375	5
GTACACT	100	5.877211E-6	16.650002	4
TAGACTG	135	2.2188033E-8	16.444445	5
GTCTTAC	90	4.445606E-5	16.444445	1
CAAGACG	295	0.0	16.305084	4
CGCAATA	115	1.2419696E-6	16.086956	36
AGACGGA	295	0.0	15.677966	6
CTATACA	95	7.0591246E-5	15.578948	4
GCGCAAG	275	0.0	15.472728	1
CGAACGA	100	1.0932787E-4	14.8	16
GGTATAC	75	0.00410422	14.8	1
AAGACGG	320	0.0	14.453126	5
CGAAAGC	285	0.0	14.280702	19
>>END_MODULE