FastQCFastQC Report
Fri 10 Feb 2017
ERR1630396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143116
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG36760.321577162772632No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG27620.24162027300816363No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT22900.20032962533986054No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA20630.18047162317735033No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT19530.170848802746178No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC17460.15274040429842642No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC16090.14075561885232996No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG14660.1282459522918059No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT14600.12772107117737833No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC13490.11801077056046805No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC13430.11748588944604048No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC13290.11626116684570945No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA12660.11074991514421982No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA12560.10987511328684053No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC12530.10961267272962674No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA12390.10838795012929571No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA12310.10768810864339227No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC12010.10506370307125434No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG11880.10392646065666128No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC11740.10270173805633025No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA11550.10103961452730956No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATA450.003825795620.5555558
ACGGACC1500.019.7333348
GGTATCA7250.018.8827571
TATATAC609.2367735E-418.53
GTATAGA701.21918994E-418.51
ATACCGT1205.173206E-918.56
TTAGACT752.067254E-417.2666664
GAGTCGT650.001579926917.0769236
GACGGAC1851.8189894E-1217.0000027
ATTCCGT1201.04115315E-716.95833229
ACCGTCG1201.04115315E-716.9583328
TATTAGA1452.9795046E-916.5862082
CAAGACG1801.0913936E-1116.4444454
TCGCCAT4850.016.40206113
TTTAGAC1151.2421042E-616.0869563
AAGACGG2100.015.8571425
CGTCGTA1201.9353793E-615.41666610
ATTAGAG1455.3445547E-815.3103463
CTTATAC2900.015.31034637
CATATAC855.364119E-415.2352943
Produced by FastQC (version 0.11.5)