##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630396.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1143116
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.472529472074577	31.0	31.0	34.0	30.0	34.0
2	31.714545155522273	31.0	31.0	34.0	30.0	34.0
3	31.822498329128454	31.0	31.0	34.0	30.0	34.0
4	35.5527068119071	37.0	35.0	37.0	33.0	37.0
5	35.41728835918664	37.0	35.0	37.0	33.0	37.0
6	35.484662099034566	37.0	35.0	37.0	33.0	37.0
7	35.465111152324	37.0	35.0	37.0	33.0	37.0
8	35.454593409592725	37.0	35.0	37.0	33.0	37.0
9	37.1629974560762	39.0	37.0	39.0	33.0	39.0
10	36.99732748032571	39.0	37.0	39.0	33.0	39.0
11	37.08321815108878	39.0	37.0	39.0	33.0	39.0
12	36.986152761399545	39.0	37.0	39.0	33.0	39.0
13	36.99471182277214	39.0	37.0	39.0	33.0	39.0
14	38.16113150371441	40.0	38.0	41.0	33.0	41.0
15	38.165039243611325	40.0	38.0	41.0	33.0	41.0
16	38.09616171937056	40.0	37.0	41.0	33.0	41.0
17	38.0557117562872	40.0	37.0	41.0	33.0	41.0
18	37.97075187470038	40.0	37.0	41.0	33.0	41.0
19	38.01800604663044	40.0	37.0	41.0	33.0	41.0
20	37.987814010126705	40.0	37.0	41.0	33.0	41.0
21	37.9086173231763	40.0	37.0	41.0	33.0	41.0
22	37.89275716550201	40.0	37.0	41.0	33.0	41.0
23	37.82543766336925	40.0	37.0	41.0	33.0	41.0
24	37.79258622921908	40.0	37.0	41.0	33.0	41.0
25	37.75928427211237	39.0	37.0	41.0	33.0	41.0
26	37.56509488100945	39.0	37.0	41.0	32.0	41.0
27	37.441144205837375	39.0	37.0	41.0	32.0	41.0
28	37.3756906560664	39.0	37.0	40.0	32.0	41.0
29	37.322361860038704	39.0	36.0	40.0	31.0	41.0
30	37.25852144489273	39.0	36.0	40.0	31.0	41.0
31	37.18971040559313	39.0	36.0	40.0	31.0	41.0
32	37.095616717813414	39.0	36.0	40.0	31.0	41.0
33	37.01410355554467	39.0	36.0	40.0	31.0	41.0
34	36.96136962477999	39.0	36.0	40.0	31.0	41.0
35	36.83462483247544	39.0	36.0	40.0	30.0	41.0
36	36.7454720255862	39.0	36.0	40.0	30.0	41.0
37	36.69042249430504	39.0	35.0	40.0	30.0	41.0
38	36.571342715874856	39.0	35.0	40.0	30.0	41.0
39	36.49080233327152	39.0	35.0	40.0	30.0	41.0
40	36.402289006540016	39.0	35.0	40.0	30.0	41.0
41	36.29591485028641	38.0	35.0	40.0	30.0	41.0
42	36.28186903166433	38.0	35.0	40.0	30.0	41.0
43	34.945805150133495	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	0.0
15	2.0
16	9.0
17	46.0
18	82.0
19	135.0
20	283.0
21	556.0
22	988.0
23	1588.0
24	2727.0
25	4006.0
26	6116.0
27	8759.0
28	12170.0
29	16659.0
30	21837.0
31	28433.0
32	36241.0
33	46746.0
34	61233.0
35	82310.0
36	114102.0
37	174953.0
38	308436.0
39	214697.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.97238075575882	18.822236763373095	12.81619713134975	26.389185349518335
2	18.73335689466336	21.13617515632709	34.689655293076115	25.44081265593343
3	20.209060147876507	22.812995356551742	29.941405771592734	27.03653872397902
4	15.789911085139217	15.654841678359851	33.08798057240035	35.467266664100585
5	14.748546954114893	36.79486596285941	33.96461951368015	14.491967569345544
6	36.03413826768237	34.03950255267182	15.338425846545759	14.587933333100054
7	29.85821211495596	30.166667249867906	20.75624870966726	19.218871925508875
8	26.6637856525497	35.06109616171937	19.54438569663971	18.73073248909122
9	26.630368221597806	14.728951392509595	18.376350256666864	40.264330129225726
10	16.88918709912205	27.409204315222603	32.54429121803911	23.157317367616233
11	36.26246155245837	21.631837888718206	20.971624926954043	21.134075631869383
12	21.509715549428055	25.225086517903694	28.43823374005788	24.826964192610372
13	30.156432068136567	19.37624877965141	25.12037273557539	25.346946416636634
14	23.23954874220989	20.205735900818464	23.74877090339038	32.80594445358126
15	25.7548665227326	27.680742811753138	21.49939288751098	25.064997778003285
16	25.928514691422393	26.28665857183348	22.769692664611465	25.01513407213266
17	24.945587324471006	26.408605950752158	23.98592968692591	24.659877037850926
18	23.675287547370523	25.24888112842441	25.820826582779	25.255004741426067
19	25.572295375097543	25.335923913233653	25.33601139341939	23.755769318249417
20	25.342047526235305	24.4430136574066	25.73150931314057	24.483429503217522
21	24.82119050035167	25.69485511531638	24.27382697818944	25.210127406142508
22	25.038928682653378	24.84069858177123	24.46698322829879	25.653389507276604
23	24.125985464292338	25.813478247177013	24.87787766070985	25.182658627820796
24	25.80280566451699	25.66484941160827	24.407496701996998	24.124848221877745
25	25.601863677876963	24.7582922468061	24.885663397240528	24.754180678076416
26	25.618484913167165	25.392086192477404	24.420356289300475	24.569072605054956
27	25.679633562997985	24.491740120862623	24.354833630182764	25.47379268595663
28	24.40522221716781	25.028168619807612	25.385262737989844	25.18134642503473
29	24.65725263227879	25.600726435462366	24.766077983336775	24.975942948922068
30	24.21136612557256	26.535627180443626	24.59251729483272	24.660489399151093
31	25.289471934606812	25.1579892154427	24.853995570003395	24.698543279947092
32	24.298233950010324	25.40503325996662	24.98311632415258	25.31361646587048
33	24.538192099489468	24.92091791209291	24.98390364582422	25.5569863425934
34	24.869567043064748	24.91969318949258	25.171461164046345	25.03927860339633
35	25.3616430878406	24.87490333439476	25.102439297499117	24.66101428026552
36	24.695481473446264	25.52365639182725	25.020032962533982	24.760829172192498
37	25.92204115767779	24.056351236444947	24.798708092617023	25.222899513260245
38	24.899222826029906	24.7557553214197	25.793007883714335	24.552013968836057
39	25.064210456331644	24.364456450613936	25.630732139170476	24.940600953883944
40	24.60021555117766	23.907110039576036	26.51926838571064	24.97340602353567
41	23.903523351960782	24.744382897273766	26.675857918181535	24.67623583258392
42	24.956259907131034	23.770291029081914	26.17004748424482	25.103401579542233
43	24.20288054755598	23.98549228599722	26.396008804005895	25.415618362440906
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13.0
1	17.0
2	21.0
3	70.5
4	120.0
5	120.0
6	194.5
7	269.0
8	256.0
9	243.0
10	366.0
11	489.0
12	489.0
13	802.5
14	1116.0
15	2109.5
16	3103.0
17	3038.5
18	2974.0
19	2974.0
20	3608.5
21	4243.0
22	4983.5
23	5724.0
24	6961.0
25	8198.0
26	8198.0
27	9822.5
28	11447.0
29	17615.0
30	23783.0
31	25319.0
32	26855.0
33	26855.0
34	31168.5
35	35482.0
36	38608.5
37	41735.0
38	52247.5
39	62760.0
40	62760.0
41	69046.5
42	75333.0
43	71513.0
44	67693.0
45	75242.0
46	82791.0
47	82791.0
48	88685.0
49	94579.0
50	98022.5
51	101466.0
52	107248.0
53	113030.0
54	113030.0
55	96773.5
56	80517.0
57	74700.5
58	68884.0
59	62235.5
60	55587.0
61	55587.0
62	51943.0
63	48299.0
64	43277.5
65	38256.0
66	33560.0
67	28864.0
68	28864.0
69	24761.5
70	20659.0
71	18261.0
72	15863.0
73	12838.0
74	9813.0
75	9813.0
76	7685.0
77	5557.0
78	4599.0
79	3641.0
80	2702.5
81	1764.0
82	1764.0
83	1329.0
84	894.0
85	695.5
86	497.0
87	393.0
88	289.0
89	289.0
90	248.5
91	208.0
92	125.0
93	42.0
94	25.5
95	9.0
96	9.0
97	7.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1143116.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.51205170585725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.69952938658427	39.6341179394618
2	10.426123323487053	10.115852675212963
3	3.0183896600512723	4.392848257704967
4	1.3994019350767728	2.715514361268844
5	0.7630220119712361	1.850788164872792
6	0.4911176975551484	1.4295076282474155
7	0.34202721080493603	1.161470921476544
8	0.23488516662521813	0.9115809078609184
9	0.19334934869606557	0.844179624111362
>10	1.1511845297637777	10.712485634157682
>50	0.1256866042990705	4.269330380726905
>100	0.13758807167893108	14.163722596914017
>500	0.012639323861595907	4.160462617812951
>1k	0.005055729544638362	3.638138290170813
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3676	0.321577162772632	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	2762	0.24162027300816363	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2290	0.20032962533986054	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2063	0.18047162317735033	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	1953	0.170848802746178	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1746	0.15274040429842642	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	1609	0.14075561885232996	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	1466	0.1282459522918059	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1460	0.12772107117737833	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	1349	0.11801077056046805	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1343	0.11748588944604048	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1329	0.11626116684570945	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1266	0.11074991514421982	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	1256	0.10987511328684053	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1253	0.10961267272962674	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1239	0.10838795012929571	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	1231	0.10768810864339227	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1201	0.10506370307125434	No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG	1188	0.10392646065666128	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	1174	0.10270173805633025	No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA	1155	0.10103961452730956	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	8.748018573793036E-5	0.0	0.0	0.0	0.0
6	8.748018573793036E-5	0.0	0.0	0.0	0.0
7	8.748018573793036E-5	0.0	0.0	0.0	0.0
8	8.748018573793036E-5	0.0	0.0	0.0	0.0
9	8.748018573793036E-5	0.0	0.0	0.0	0.0
10	8.748018573793036E-5	0.0	0.0	0.0	0.0
11	8.748018573793036E-5	0.0	0.0	0.0	0.0
12	8.748018573793036E-5	0.0	0.0	0.0	0.0
13	8.748018573793036E-5	0.0	0.0	0.0	0.0
14	8.748018573793036E-5	0.0	0.0	0.0	0.0
15	8.748018573793036E-5	0.0	0.0	8.748018573793036E-5	0.0
16	8.748018573793036E-5	0.0	0.0	8.748018573793036E-5	0.0
17	8.748018573793036E-5	0.0	0.0	8.748018573793036E-5	0.0
18	8.748018573793036E-5	0.0	0.0	1.7496037147586072E-4	0.0
19	8.748018573793036E-5	0.0	0.0	1.7496037147586072E-4	0.0
20	8.748018573793036E-5	0.0	0.0	2.624405572137911E-4	0.0
21	8.748018573793036E-5	0.0	0.0	4.3740092868965177E-4	0.0
22	8.748018573793036E-5	0.0	0.0	5.248811144275821E-4	0.0
23	8.748018573793036E-5	0.0	0.0	0.0011372424145930946	0.0
24	8.748018573793036E-5	0.0	0.0	0.0018370839004965376	0.0
25	8.748018573793036E-5	0.0	0.0	0.0020995244577103286	0.0
26	8.748018573793036E-5	0.0	0.0	0.0027993659436137716	0.0
27	8.748018573793036E-5	0.0	0.0	0.005773692258703404	0.0
28	8.748018573793036E-5	0.0	0.0	0.015396512689875743	0.0
29	8.748018573793036E-5	0.0	0.0	0.03359239132336526	0.0
30	8.748018573793036E-5	0.0	0.0	0.05852424425867541	0.0
31	8.748018573793036E-5	0.0	0.0	0.16113850212926772	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGATA	45	0.0038257956	20.555555	8
ACGGACC	150	0.0	19.733334	8
GGTATCA	725	0.0	18.882757	1
TATATAC	60	9.2367735E-4	18.5	3
GTATAGA	70	1.21918994E-4	18.5	1
ATACCGT	120	5.173206E-9	18.5	6
TTAGACT	75	2.067254E-4	17.266666	4
GAGTCGT	65	0.0015799269	17.076923	6
GACGGAC	185	1.8189894E-12	17.000002	7
ATTCCGT	120	1.04115315E-7	16.958332	29
ACCGTCG	120	1.04115315E-7	16.958332	8
TATTAGA	145	2.9795046E-9	16.586208	2
CAAGACG	180	1.0913936E-11	16.444445	4
TCGCCAT	485	0.0	16.402061	13
TTTAGAC	115	1.2421042E-6	16.086956	3
AAGACGG	210	0.0	15.857142	5
CGTCGTA	120	1.9353793E-6	15.416666	10
ATTAGAG	145	5.3445547E-8	15.310346	3
CTTATAC	290	0.0	15.310346	37
CATATAC	85	5.364119E-4	15.235294	3
>>END_MODULE