FastQCFastQC Report
Fri 10 Feb 2017
ERR1630395.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630395.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251527
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54950.2440565891503855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53970.23970398756044234No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40580.18023323726519824No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23510.10441802385669816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23650.019.0084571
GTTAGAC1205.180482E-918.53
CGCAATA2050.017.14634136
ATACCGT2450.016.6122446
CGAACGA1251.6596641E-716.27999916
ACGAACG1251.6596641E-716.27999915
TACCGTC2050.016.2439027
CTAGCGG2200.015.97727329
GTATAGA2900.015.9482761
ACCGTCG2350.015.7446798
TCTAGCG2201.8189894E-1215.13636428
TTAGGAC9550.014.9162313
CCGATAA1252.961613E-614.7999999
GTATAGG2400.014.6458341
TAGGACA8850.014.6327684
GCTTAGG8500.014.5823541
CTTAGGA9350.014.443852
TTAGACT2700.014.3888884
AATCGTG908.2783593E-414.3888887
ATCTCGC4550.014.23076911
Produced by FastQC (version 0.11.5)