##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630395.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2251527
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90088593208076	33.0	31.0	34.0	30.0	34.0
2	32.10958829274532	33.0	31.0	34.0	30.0	34.0
3	32.24015346029606	34.0	31.0	34.0	30.0	34.0
4	35.86459767082518	37.0	35.0	37.0	35.0	37.0
5	35.795217201481485	37.0	35.0	37.0	35.0	37.0
6	35.84742798998191	37.0	35.0	37.0	35.0	37.0
7	35.826117563768946	37.0	35.0	37.0	35.0	37.0
8	35.82538428364394	37.0	35.0	37.0	35.0	37.0
9	37.57947606224575	39.0	37.0	39.0	35.0	39.0
10	37.46608146382433	39.0	37.0	39.0	35.0	39.0
11	37.5387130600699	39.0	37.0	39.0	35.0	39.0
12	37.46568928553822	39.0	37.0	39.0	35.0	39.0
13	37.468571773734006	39.0	37.0	39.0	35.0	39.0
14	38.73056507872213	40.0	38.0	41.0	35.0	41.0
15	38.74743274231222	40.0	38.0	41.0	35.0	41.0
16	38.70650673964825	40.0	38.0	41.0	35.0	41.0
17	38.678797989098065	40.0	38.0	41.0	34.0	41.0
18	38.631132560257996	40.0	38.0	41.0	34.0	41.0
19	38.67004304190001	40.0	38.0	41.0	34.0	41.0
20	38.64916965241812	40.0	38.0	41.0	34.0	41.0
21	38.593700630727504	40.0	38.0	41.0	34.0	41.0
22	38.566632778554286	40.0	38.0	41.0	34.0	41.0
23	38.51188415684111	40.0	38.0	41.0	34.0	41.0
24	38.486327279219836	40.0	38.0	41.0	34.0	41.0
25	38.466473642110444	40.0	38.0	41.0	34.0	41.0
26	38.31289698058251	40.0	38.0	41.0	34.0	41.0
27	38.19990344330758	40.0	38.0	41.0	34.0	41.0
28	38.14086217931209	40.0	38.0	41.0	33.0	41.0
29	38.08913239770165	40.0	38.0	41.0	33.0	41.0
30	38.02469834916481	40.0	38.0	41.0	33.0	41.0
31	37.969633497621835	40.0	37.0	41.0	33.0	41.0
32	37.87901144423318	40.0	37.0	41.0	33.0	41.0
33	37.79943789259467	40.0	37.0	41.0	33.0	41.0
34	37.74506634830495	40.0	37.0	41.0	33.0	41.0
35	37.62748570192585	40.0	37.0	41.0	32.0	41.0
36	37.545242406597836	40.0	37.0	41.0	32.0	41.0
37	37.48895438517948	40.0	37.0	41.0	32.0	41.0
38	37.38131321543113	40.0	37.0	41.0	32.0	41.0
39	37.30466345728921	40.0	36.0	41.0	31.0	41.0
40	37.21404851018886	40.0	36.0	41.0	31.0	41.0
41	37.09650250696527	39.0	36.0	41.0	31.0	41.0
42	37.06115938205493	39.0	36.0	41.0	31.0	41.0
43	35.85477367137947	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	5.0
11	5.0
12	3.0
13	5.0
14	3.0
15	8.0
16	12.0
17	24.0
18	84.0
19	146.0
20	278.0
21	605.0
22	1153.0
23	2013.0
24	3348.0
25	5241.0
26	7844.0
27	11716.0
28	16963.0
29	23484.0
30	32086.0
31	42666.0
32	55350.0
33	72439.0
34	96186.0
35	129402.0
36	181168.0
37	278091.0
38	525229.0
39	765959.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.653274866346266	19.075898268153125	12.188794538106805	26.08203232739381
2	17.880709402996278	21.400187517182783	35.20388607376238	25.515217006058556
3	19.34362768023657	23.377956382490638	30.396348789066263	26.882067148206527
4	14.270448455648099	15.788573710197568	35.35835901590343	34.58261881825091
5	14.063788708729675	37.00697348954732	34.79860556857635	14.13063223314666
6	33.84174384761986	35.78122758465699	16.19865095999293	14.17837760773022
7	28.82545934381422	31.178617889103705	21.566341420733572	18.4295813463485
8	26.871007987023916	34.4261028182207	20.359826908582487	18.343062286172895
9	26.640275688454988	14.452236193481136	19.622371839200685	39.28511627886319
10	16.184571626278522	27.3184376647493	33.91880266148263	22.578188047489547
11	35.077127656030775	22.25072139929923	21.879684320907543	20.79246662376245
12	20.934903290078243	25.710684348888552	30.216071137499128	23.138341223534074
13	29.64619122933014	20.110218531689828	25.640820651939773	24.602769587040264
14	22.420694932816705	20.87076903807949	25.67484200722443	31.03369402187937
15	24.617737206793432	28.08769337431885	22.794441283626625	24.500128135261093
16	24.405259186321103	27.17298082590171	24.225114777659783	24.196645210117403
17	23.061104752463553	27.055504997275182	25.994580566877502	23.888809683383766
18	22.923598073662895	26.041082341006792	27.176711627264517	23.858607958065793
19	23.860695430256886	26.055428160532827	27.145443958700028	22.938432450510255
20	24.18736262101232	25.523566894822935	27.0136667248494	23.275403759315346
21	24.049100899078717	25.95913795393082	26.162555456807755	23.829205690182707
22	24.093293129507217	25.673465163864346	26.365173502249807	23.868068204378627
23	23.53176311010261	26.05089790173513	26.49313110613375	23.924207882028508
24	23.871532519929808	26.154472053855006	26.534614064144023	23.439381362071163
25	24.119986124972073	25.67661857930196	26.438590343353646	23.764804952372323
26	23.788611018211196	26.249341002794992	26.374811405770394	23.587236573223418
27	24.033822379211976	25.793783507814915	26.105305421609426	24.067088691363683
28	23.530963652667722	25.98507590626273	26.722131246927084	23.761829194142464
29	23.2469786060749	26.297574934699874	26.763747447843173	23.691699011382052
30	23.21775399539957	26.968053236758877	26.541142966528934	23.27304980131262
31	23.943217203258055	26.337814292255878	26.28309587226802	23.435872632218047
32	23.100633481188545	26.334172319496947	26.55615500058405	24.009039198730463
33	23.246756534565208	26.08793942955159	26.821796940476396	23.843507095406807
34	23.773154841136705	25.74603813323136	26.751267028998544	23.729539996633395
35	23.832403519922256	25.945280691726104	26.884643177718942	23.337672610632694
36	23.302007926176323	26.362641887039327	26.635523358147605	23.699826828636745
37	24.03568777989338	25.5929420344504	26.5794725091016	23.791897676554623
38	23.502671742333092	25.896114059480524	27.136916412727896	23.46429778545849
39	23.516395761631994	25.698337172949735	27.090148152786973	23.695118912631294
40	23.082690103205515	25.382285000357534	27.71305873747017	23.82196615896678
41	22.63548249698982	25.581660801758094	27.988915966808303	23.79394073444378
42	23.211136264410776	25.225236028704074	27.730646801037693	23.832980905847453
43	22.77800799190949	25.00009993217936	27.918696955444016	24.303195120467134
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	210.0
1	216.0
2	222.0
3	577.0
4	932.0
5	932.0
6	1304.0
7	1676.0
8	1585.0
9	1494.0
10	2180.5
11	2867.0
12	2867.0
13	5074.0
14	7281.0
15	10955.0
16	14629.0
17	14662.0
18	14695.0
19	14695.0
20	18131.5
21	21568.0
22	23214.0
23	24860.0
24	29355.5
25	33851.0
26	33851.0
27	39145.0
28	44439.0
29	53722.5
30	63006.0
31	70137.0
32	77268.0
33	77268.0
34	84783.0
35	92298.0
36	100081.5
37	107865.0
38	117257.5
39	126650.0
40	126650.0
41	133262.0
42	139874.0
43	137409.5
44	134945.0
45	141388.0
46	147831.0
47	147831.0
48	150797.5
49	153764.0
50	158179.0
51	162594.0
52	168240.0
53	173886.0
54	173886.0
55	161754.5
56	149623.0
57	141900.5
58	134178.0
59	121867.5
60	109557.0
61	109557.0
62	100398.0
63	91239.0
64	80064.5
65	68890.0
66	59818.0
67	50746.0
68	50746.0
69	42919.0
70	35092.0
71	30521.5
72	25951.0
73	20963.5
74	15976.0
75	15976.0
76	12575.0
77	9174.0
78	7556.5
79	5939.0
80	4447.0
81	2955.0
82	2955.0
83	2177.5
84	1400.0
85	1167.5
86	935.0
87	792.5
88	650.0
89	650.0
90	530.5
91	411.0
92	240.0
93	69.0
94	46.0
95	23.0
96	23.0
97	15.5
98	8.0
99	7.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2251527.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.28655811243744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.38436844671305	50.02858763697385
2	9.689306648515355	11.48891283372864
3	2.5048390543764985	4.455098584787853
4	1.0333940465702	2.450655047801242
5	0.5503854751675169	1.6315230228880238
6	0.323380200247949	1.150325942064701
7	0.2328352776837303	0.9662801554715389
8	0.17211284627552356	0.8163182610088671
9	0.12282271590431598	0.655356247558976
>10	0.8072954446552628	9.215119131144553
>50	0.09073368292162333	3.742616288031485
>100	0.08117712566329835	9.504813514905802
>500	0.006299173119276629	2.5564417041762146
>1k	8.998818741823568E-4	0.8537033026137092
>5k	1.4998031236372614E-4	0.4842483268445225
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5495	0.2440565891503855	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5397	0.23970398756044234	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4058	0.18023323726519824	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2351	0.10441802385669816	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	4.4414301938195724E-5	0.0	0.0	0.0	0.0
11	4.4414301938195724E-5	0.0	0.0	0.0	0.0
12	4.4414301938195724E-5	0.0	0.0	4.4414301938195724E-5	0.0
13	4.4414301938195724E-5	0.0	0.0	4.4414301938195724E-5	0.0
14	4.4414301938195724E-5	0.0	0.0	1.3324290581458718E-4	0.0
15	4.4414301938195724E-5	0.0	0.0	1.3324290581458718E-4	4.4414301938195724E-5
16	4.4414301938195724E-5	0.0	0.0	3.553144155055658E-4	4.4414301938195724E-5
17	4.4414301938195724E-5	0.0	0.0	7.106288310111316E-4	4.4414301938195724E-5
18	4.4414301938195724E-5	0.0	0.0	8.882860387639145E-4	4.4414301938195724E-5
19	4.4414301938195724E-5	0.0	0.0	0.0010215289445785016	4.4414301938195724E-5
20	4.4414301938195724E-5	0.0	0.0	0.001288014756207676	4.4414301938195724E-5
21	4.4414301938195724E-5	0.0	0.0	0.0015545005678368503	4.4414301938195724E-5
22	4.4414301938195724E-5	0.0	0.0	0.002220715096909786	4.4414301938195724E-5
23	4.4414301938195724E-5	0.0	0.0	0.0032866583434264835	4.4414301938195724E-5
24	4.4414301938195724E-5	0.0	0.0	0.005196473326768899	4.4414301938195724E-5
25	4.4414301938195724E-5	0.0	0.0	0.006306830875223793	4.4414301938195724E-5
26	4.4414301938195724E-5	0.0	0.0	0.0083054744624426	4.4414301938195724E-5
27	4.4414301938195724E-5	0.0	0.0	0.015633834282244893	4.4414301938195724E-5
28	4.4414301938195724E-5	0.0	0.0	0.03508729853117462	4.4414301938195724E-5
29	4.4414301938195724E-5	0.0	0.0	0.06271299433673236	4.4414301938195724E-5
30	4.4414301938195724E-5	0.0	0.0	0.10326325200630505	4.4414301938195724E-5
31	4.4414301938195724E-5	0.0	0.0	0.22562465384603428	4.4414301938195724E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2365	0.0	19.008457	1
GTTAGAC	120	5.180482E-9	18.5	3
CGCAATA	205	0.0	17.146341	36
ATACCGT	245	0.0	16.612244	6
CGAACGA	125	1.6596641E-7	16.279999	16
ACGAACG	125	1.6596641E-7	16.279999	15
TACCGTC	205	0.0	16.243902	7
CTAGCGG	220	0.0	15.977273	29
GTATAGA	290	0.0	15.948276	1
ACCGTCG	235	0.0	15.744679	8
TCTAGCG	220	1.8189894E-12	15.136364	28
TTAGGAC	955	0.0	14.916231	3
CCGATAA	125	2.961613E-6	14.799999	9
GTATAGG	240	0.0	14.645834	1
TAGGACA	885	0.0	14.632768	4
GCTTAGG	850	0.0	14.582354	1
CTTAGGA	935	0.0	14.44385	2
TTAGACT	270	0.0	14.388888	4
AATCGTG	90	8.2783593E-4	14.388888	7
ATCTCGC	455	0.0	14.230769	11
>>END_MODULE