FastQCFastQC Report
Fri 10 Feb 2017
ERR1630394.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630394.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529498
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55320.3616872987084651No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51630.3375617359421196No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37400.24452467410875986No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23650.15462589686289227No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC17830.1165741962395505No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT17260.11284748329190362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG3950.017.7974684
CGCGCTT1054.799822E-717.61904712
CGAGCCG2050.017.14634315
GCGTAAC3500.016.91428611
CGCGTAA3550.016.67605810
GGTATCA21100.016.4834121
CGGAATA1352.2208042E-816.44444328
CGGACCA4150.016.0481939
CCGTGTA700.002593018815.8571439
CGAATTA700.002593018815.85714315
AAGACGG4200.015.8571425
GGTCGCG4150.015.1566277
CGCAAGA4150.015.1566272
GTCGCGT4050.015.0740748
TCGCGTA3950.014.9873429
GACGGAC4200.014.976197
ACGGACC4450.014.9662938
GTTCGCG1252.9601106E-614.79999930
CGTAACT4050.014.61728412
CGCCCGA1405.998063E-714.53571510
Produced by FastQC (version 0.11.5)