##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630394.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1529498
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.61891941015941	31.0	30.0	33.0	27.0	34.0
2	30.852560774842466	31.0	31.0	34.0	27.0	34.0
3	30.961614856639237	31.0	31.0	34.0	27.0	34.0
4	34.799657796218106	35.0	35.0	37.0	32.0	37.0
5	34.19270832652282	35.0	35.0	37.0	30.0	37.0
6	34.31393045299831	35.0	35.0	37.0	30.0	37.0
7	34.33509556730378	35.0	35.0	37.0	30.0	37.0
8	34.29159828911185	35.0	35.0	37.0	30.0	37.0
9	35.83064116461741	37.0	35.0	39.0	30.0	39.0
10	35.49753121612451	37.0	35.0	39.0	30.0	39.0
11	35.71828926876662	37.0	35.0	39.0	30.0	39.0
12	35.52480683204555	37.0	35.0	39.0	30.0	39.0
13	35.64508812695407	37.0	35.0	39.0	30.0	39.0
14	36.5054684608937	38.0	35.0	40.0	31.0	41.0
15	36.44139645818432	38.0	35.0	40.0	31.0	41.0
16	36.39087138394427	38.0	35.0	40.0	30.0	41.0
17	36.312312275007876	38.0	35.0	40.0	30.0	41.0
18	36.238163109726194	38.0	35.0	40.0	30.0	41.0
19	36.24001404382353	38.0	35.0	40.0	30.0	41.0
20	36.17686848887674	38.0	34.0	40.0	30.0	41.0
21	36.12226625991011	38.0	34.0	40.0	30.0	41.0
22	36.046292312902665	38.0	34.0	40.0	29.0	41.0
23	35.95735986578603	38.0	34.0	40.0	29.0	41.0
24	35.90787630974346	38.0	34.0	40.0	29.0	41.0
25	35.86272881690594	38.0	34.0	40.0	29.0	41.0
26	35.604491800577705	38.0	34.0	40.0	27.0	41.0
27	35.461338295309965	38.0	34.0	40.0	27.0	41.0
28	35.31233711976086	38.0	34.0	40.0	27.0	41.0
29	35.18314963471675	38.0	34.0	40.0	27.0	41.0
30	35.076955968559616	38.0	33.0	40.0	27.0	40.0
31	34.93561416883186	37.0	33.0	40.0	26.0	40.0
32	34.78258422044357	37.0	33.0	39.0	26.0	40.0
33	34.67755629624884	37.0	33.0	39.0	25.0	40.0
34	34.53113243691721	37.0	33.0	39.0	25.0	40.0
35	34.40057718284038	37.0	33.0	39.0	25.0	40.0
36	34.26698236937871	37.0	33.0	39.0	24.0	40.0
37	34.163024730990166	37.0	32.0	39.0	24.0	40.0
38	34.0069689532121	37.0	32.0	39.0	24.0	40.0
39	33.863265594332255	37.0	32.0	39.0	24.0	40.0
40	33.69694435690665	37.0	31.0	39.0	23.0	40.0
41	33.50372998199409	36.0	31.0	39.0	23.0	40.0
42	33.41053535212207	36.0	31.0	39.0	22.0	40.0
43	32.157806679054175	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	7.0
11	5.0
12	13.0
13	15.0
14	42.0
15	54.0
16	133.0
17	255.0
18	496.0
19	858.0
20	1523.0
21	2610.0
22	4212.0
23	6310.0
24	9684.0
25	13905.0
26	19340.0
27	26287.0
28	34443.0
29	44698.0
30	56692.0
31	71472.0
32	88914.0
33	112399.0
34	142743.0
35	181871.0
36	225469.0
37	242942.0
38	186692.0
39	55408.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.20750729978071	20.81447638375467	12.853956003865322	25.1240603125993
2	20.112350588232218	22.1942755073887	33.03410661537315	24.659267289005935
3	21.312156014587792	23.65802374373814	29.267315158306843	25.76250508336722
4	16.13581711123519	16.50574240698582	33.656729201345804	33.70171128043319
5	15.108094289760432	36.96990777366169	33.101579734004225	14.820418202573654
6	34.620574855279315	35.017698617454876	15.451082642801756	14.910643884464053
7	29.247897022421736	30.933809655194054	21.007284743098715	18.81100857928549
8	27.67849320496006	33.24247563579684	19.779169374526806	19.299861784716292
9	27.24835207368692	13.88932839402209	19.87227181728907	38.990047715001914
10	17.56138288510348	26.700329127596113	32.55571435856732	23.18257362873309
11	36.02724554069374	21.7033301122329	21.010488408615114	21.25893593845824
12	21.670181981277516	25.773881364996882	28.934656992032682	23.62127966169292
13	30.38343299566263	19.690316692143437	24.751650541550234	25.174599770643702
14	22.758120638274782	21.30234887525188	25.318699337952715	30.620831148520626
15	25.824093918396755	27.67287044507414	22.1491626664435	24.35387297008561
16	24.908434009067026	26.348841253796994	23.853316578380618	24.889408158755355
17	23.5190238888838	26.5605447015949	25.64050427002847	24.279927139492827
18	23.68862201846619	24.682150614123067	26.921120524511966	24.70810684289878
19	24.157991707082978	25.43631962905476	26.649593526764992	23.75609513709727
20	24.525955574966428	25.00343249876757	26.461361832444368	24.00925009382163
21	25.70411991385409	24.946224185974746	25.759824465282072	23.589831434889096
22	25.062471477569765	25.182510863041337	26.175581792195874	23.579435867193027
23	24.429976371332295	25.1649233931656	26.060772881036787	24.34432735446532
24	24.423765183086214	25.50850017456708	26.280191278445606	23.787543363901097
25	24.658221194143437	25.09267746672438	26.169697508594325	24.079403830537863
26	24.566884036461637	25.596764428590298	26.230109486903547	23.60624204804452
27	24.97054589152781	25.162700441582793	25.912685077064502	23.9540685898249
28	24.10097953707687	25.09097756257282	26.684833847445372	24.12320905290494
29	23.755898994310552	25.31072286462617	27.037694720751514	23.895683420311762
30	23.84782458035251	25.822394014245198	26.66927318636572	23.660508219036572
31	24.33079350218176	25.80761792431242	25.97649686367684	23.885091709828977
32	23.767732942442553	25.5871534320411	26.14962556342015	24.49548806209619
33	23.838540488447844	25.416770731311843	26.61624925302289	24.128439527217427
34	24.63350720301694	25.21964723065999	26.354333251825107	23.79251231449796
35	24.480842734021227	25.121510456371958	26.88372263317768	23.51392417642913
36	24.12438590962525	25.973685483733878	25.744917613491484	24.157010993149385
37	24.240240915646833	25.356620276718246	26.414614468276522	23.988524339358406
38	24.10228715565499	25.399967832582977	26.653581763428264	23.844163248333768
39	23.906667416367984	25.161458203933577	26.709613219500778	24.222261160197657
40	23.726608338160627	24.831284512957847	27.503206934562847	23.938900214318686
41	23.207941429148647	25.1058190334345	27.69379234232408	23.99244719509277
42	23.700521347527097	25.341778805856563	27.092941605677158	23.864758240939185
43	23.627229326223375	24.32412464743334	27.41376582381932	24.634880202523966
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	270.0
1	248.0
2	226.0
3	501.0
4	776.0
5	776.0
6	1148.0
7	1520.0
8	1459.0
9	1398.0
10	1851.5
11	2305.0
12	2305.0
13	4552.0
14	6799.0
15	9713.0
16	12627.0
17	12176.0
18	11725.0
19	11725.0
20	14332.0
21	16939.0
22	16161.0
23	15383.0
24	18158.0
25	20933.0
26	20933.0
27	24411.5
28	27890.0
29	32212.0
30	36534.0
31	41740.5
32	46947.0
33	46947.0
34	52843.0
35	58739.0
36	64721.5
37	70704.0
38	74021.0
39	77338.0
40	77338.0
41	81798.5
42	86259.0
43	87559.5
44	88860.0
45	91077.0
46	93294.0
47	93294.0
48	96029.5
49	98765.0
50	99266.0
51	99767.0
52	104492.0
53	109217.0
54	109217.0
55	107131.5
56	105046.0
57	101795.0
58	98544.0
59	90676.0
60	82808.0
61	82808.0
62	77529.0
63	72250.0
64	63513.5
65	54777.0
66	46497.5
67	38218.0
68	38218.0
69	31940.0
70	25662.0
71	22761.5
72	19861.0
73	18757.5
74	17654.0
75	17654.0
76	15761.0
77	13868.0
78	11153.5
79	8439.0
80	5947.0
81	3455.0
82	3455.0
83	2504.5
84	1554.0
85	1212.0
86	870.0
87	774.0
88	678.0
89	678.0
90	558.0
91	438.0
92	275.5
93	113.0
94	71.5
95	30.0
96	30.0
97	21.0
98	12.0
99	9.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1529498.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.2177799660507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.90082393197298	51.97439958403933
2	9.711250890000217	11.890024403582947
3	2.4210327121707746	4.446307435928443
4	0.8862065962818936	2.1700640166259078
5	0.4751645590152938	1.4544259710731884
6	0.2663579846626311	0.9783506698366594
7	0.18465821455912293	0.7913056163462917
8	0.14501487736042604	0.7101991083243511
9	0.11605377625670805	0.6394099085201189
>10	0.7247723481885571	8.800287504383437
>50	0.09105433156434588	3.8862635278648825
>100	0.07170595308544055	8.616449616650291
>500	0.005045086717025257	2.1493919618603474
>1k	6.440536234500327E-4	0.7903248991193372
>5k	2.1468454115001096E-4	0.7027957758444592
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5532	0.3616872987084651	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5163	0.3375617359421196	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3740	0.24452467410875986	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2365	0.15462589686289227	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1783	0.1165741962395505	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	1726	0.11284748329190362	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	6.538092890608553E-5
11	0.0	0.0	0.0	6.538092890608553E-5	6.538092890608553E-5
12	0.0	0.0	0.0	6.538092890608553E-5	6.538092890608553E-5
13	0.0	0.0	0.0	6.538092890608553E-5	6.538092890608553E-5
14	0.0	0.0	0.0	6.538092890608553E-5	6.538092890608553E-5
15	0.0	0.0	0.0	1.3076185781217106E-4	1.3076185781217106E-4
16	6.538092890608553E-5	0.0	0.0	3.269046445304276E-4	1.9614278671825657E-4
17	6.538092890608553E-5	0.0	0.0	5.884283601547697E-4	1.9614278671825657E-4
18	1.3076185781217106E-4	0.0	0.0	8.499520757791119E-4	1.9614278671825657E-4
19	1.3076185781217106E-4	0.0	0.0	0.001242237649215625	1.9614278671825657E-4
20	1.9614278671825657E-4	0.0	0.0	0.0014383804359338816	2.615237156243421E-4
21	1.9614278671825657E-4	0.0	0.0	0.0021575706539008225	3.269046445304276E-4
22	1.9614278671825657E-4	0.0	0.0	0.0036613320187407895	3.269046445304276E-4
23	1.9614278671825657E-4	0.0	0.0	0.005361236170299013	3.269046445304276E-4
24	1.9614278671825657E-4	0.0	0.0	0.00934947283357023	3.269046445304276E-4
25	2.615237156243421E-4	0.0	0.0	0.010787853269504112	3.269046445304276E-4
26	2.615237156243421E-4	0.0	0.0	0.012945423923404933	3.269046445304276E-4
27	2.615237156243421E-4	0.0	0.0	0.01797975544917352	3.269046445304276E-4
28	2.615237156243421E-4	0.0	0.0	0.03314813095538536	3.269046445304276E-4
29	2.615237156243421E-4	6.538092890608553E-5	0.0	0.05243550498268059	3.269046445304276E-4
30	3.269046445304276E-4	6.538092890608553E-5	0.0	0.08545287408025379	3.269046445304276E-4
31	3.269046445304276E-4	6.538092890608553E-5	0.0	0.1910430742635819	3.269046445304276E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGACG	395	0.0	17.797468	4
CGCGCTT	105	4.799822E-7	17.619047	12
CGAGCCG	205	0.0	17.146343	15
GCGTAAC	350	0.0	16.914286	11
CGCGTAA	355	0.0	16.676058	10
GGTATCA	2110	0.0	16.483412	1
CGGAATA	135	2.2208042E-8	16.444443	28
CGGACCA	415	0.0	16.048193	9
CCGTGTA	70	0.0025930188	15.857143	9
CGAATTA	70	0.0025930188	15.857143	15
AAGACGG	420	0.0	15.857142	5
GGTCGCG	415	0.0	15.156627	7
CGCAAGA	415	0.0	15.156627	2
GTCGCGT	405	0.0	15.074074	8
TCGCGTA	395	0.0	14.987342	9
GACGGAC	420	0.0	14.97619	7
ACGGACC	445	0.0	14.966293	8
GTTCGCG	125	2.9601106E-6	14.799999	30
CGTAACT	405	0.0	14.617284	12
CGCCCGA	140	5.998063E-7	14.535715	10
>>END_MODULE