FastQCFastQC Report
Fri 10 Feb 2017
ERR1630393.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630393.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1553346
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG18050.11620076917827708No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15680.10094338286511827No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG450.003826404720.5555555
ATATCGC555.1442115E-420.1818186
GGTATCA7800.019.9230771
TATTTCG801.6172664E-518.529
GCTCGTT801.6172664E-518.511
TGTCGCG500.00703596818.522
ATGCGTT953.6080364E-617.52631636
TACGTTA752.0678557E-417.26666619
GTGTAGA1401.869921E-917.1785721
TCGTTTA1655.4569682E-1116.81818230
GTGTTAC1352.2208042E-816.4444451
GTATGTA2050.016.2439041
ACCGCGA803.3835223E-416.187536
AATTACG803.3835223E-416.187516
CGCAAGA3550.016.154932
AAGACGG3450.016.0869565
GCGCAAG3800.016.065791
CGAACGA1403.475361E-815.85714216
CGGACCA3300.015.696979
GACGGTA1902.7284841E-1115.5789488
Produced by FastQC (version 0.11.5)