##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630393.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1553346
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22009648848357	31.0	31.0	34.0	28.0	34.0
2	31.44364681146377	31.0	31.0	34.0	28.0	34.0
3	31.580307928819465	31.0	31.0	34.0	30.0	34.0
4	35.36278137646088	37.0	35.0	37.0	33.0	37.0
5	35.119021776217274	37.0	35.0	37.0	32.0	37.0
6	35.183633266509844	37.0	35.0	37.0	32.0	37.0
7	35.1769766684306	37.0	35.0	37.0	32.0	37.0
8	35.12836933947749	37.0	35.0	37.0	32.0	37.0
9	36.7641626527509	38.0	37.0	39.0	33.0	39.0
10	36.56470483717086	38.0	35.0	39.0	32.0	39.0
11	36.69038063638108	38.0	35.0	39.0	32.0	39.0
12	36.54049001317156	38.0	35.0	39.0	32.0	39.0
13	36.59130805371115	38.0	35.0	39.0	32.0	39.0
14	37.66685593550954	39.0	37.0	40.0	33.0	41.0
15	37.62953520979872	39.0	37.0	40.0	32.0	41.0
16	37.6007779335705	39.0	37.0	40.0	32.0	41.0
17	37.527277245378684	39.0	36.0	40.0	32.0	41.0
18	37.481505086439206	39.0	36.0	40.0	32.0	41.0
19	37.4839063544117	39.0	37.0	40.0	32.0	41.0
20	37.42080128960322	39.0	36.0	40.0	32.0	41.0
21	37.3616167936828	39.0	36.0	40.0	32.0	41.0
22	37.33200136994591	39.0	36.0	40.0	32.0	41.0
23	37.26911518747272	39.0	36.0	40.0	32.0	41.0
24	37.242478494810555	39.0	36.0	40.0	32.0	41.0
25	37.20454039215989	39.0	36.0	40.0	31.0	41.0
26	37.00410597510149	39.0	36.0	40.0	31.0	41.0
27	36.892944649807575	39.0	36.0	40.0	31.0	41.0
28	36.80389365923625	39.0	36.0	40.0	30.0	41.0
29	36.71106437329481	39.0	36.0	40.0	30.0	41.0
30	36.64858183559877	39.0	36.0	40.0	30.0	41.0
31	36.523593584429996	38.0	35.0	40.0	30.0	41.0
32	36.4080494622576	38.0	35.0	40.0	30.0	41.0
33	36.35885823248652	38.0	35.0	40.0	30.0	41.0
34	36.23877552071464	38.0	35.0	40.0	30.0	41.0
35	36.14579301713849	38.0	35.0	40.0	29.0	41.0
36	36.04905732528361	38.0	35.0	40.0	29.0	41.0
37	35.989290216088364	38.0	35.0	40.0	29.0	41.0
38	35.86906265571225	38.0	35.0	40.0	29.0	41.0
39	35.76239356846446	38.0	34.0	40.0	28.0	41.0
40	35.66073688669491	38.0	34.0	40.0	27.0	41.0
41	35.52658776602251	38.0	34.0	40.0	27.0	41.0
42	35.482968379227806	38.0	34.0	40.0	27.0	41.0
43	34.20570626248112	37.0	32.0	40.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	2.0
12	1.0
13	4.0
14	9.0
15	18.0
16	28.0
17	79.0
18	146.0
19	321.0
20	610.0
21	1124.0
22	1945.0
23	3173.0
24	4872.0
25	7414.0
26	10693.0
27	15363.0
28	20978.0
29	28082.0
30	36253.0
31	47142.0
32	59375.0
33	76645.0
34	99307.0
35	133352.0
36	185727.0
37	275245.0
38	380479.0
39	164954.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.673394079619094	19.280443635867346	12.563459782945976	26.482702501567584
2	18.769932777372205	21.32577030487734	33.931976520363136	25.972320397387318
3	20.111745869883464	22.688570350713878	30.025313098305205	27.17437068109745
4	15.409058896086256	15.790429176757787	34.14500053433041	34.65551139282555
5	14.678700044935255	36.605302360195346	34.33845389243607	14.377543702433329
6	35.412071747054426	35.12218140710441	15.348351236620816	14.117395609220354
7	30.122844491826033	30.544836758841882	20.576806455226333	18.755512294105756
8	27.649731611630635	34.117640242418624	19.629367829189377	18.603260316761368
9	27.215378930386404	14.086558950806838	18.45403406581663	40.244028052990124
10	16.691065609336235	26.99018763366307	32.91050416327077	23.408242593729923
11	36.78060135990307	21.322358315533048	20.895022744449722	21.002017580114156
12	21.819028085178704	24.95947457939184	28.940622372607262	24.280874962822192
13	30.52352791972941	19.026025109666488	24.906556555976582	25.54389041462752
14	23.223222643248832	19.95749820065845	24.264330033360242	32.554949122732474
15	25.582967349193293	27.33827492393839	21.452786436505452	25.62597129036287
16	25.75788008595638	26.31210303435294	22.8495132443126	25.080503635378083
17	24.39701135484303	26.19648165959162	24.64634408560617	24.760162899959184
18	24.35020916138452	25.1658033689854	25.659318657916526	24.82466881171355
19	25.374385359089345	25.016899003827863	25.45356926273992	24.15514637434287
20	25.622430546703693	24.439049638651014	25.18382897306846	24.754690841576828
21	25.41777556320356	25.17269172483143	24.380144539593886	25.029388172371124
22	25.559340932413	24.82775891527065	24.591494747467724	25.02140540484863
23	24.726493646618334	25.04181296375695	24.900118840232633	25.331574549392087
24	25.211124887822805	25.297905296051233	24.805420041639145	24.685549774486816
25	25.583482366452802	24.628189727208234	24.85756553916513	24.93076236717383
26	25.24962242797162	25.377732971276203	24.642803341946998	24.729841258805184
27	25.6217867751293	24.638876335343188	24.424886664014327	25.31445022551318
28	24.8237675315094	25.019345335810566	25.08919455163241	25.067692581047623
29	24.51655973620816	25.280459086385132	25.1235719537051	25.07940922370161
30	24.618790662222068	25.998779409094947	24.925676571736112	24.456753356946876
31	25.410179058625705	25.263141631033907	24.549649595132056	24.777029715208332
32	24.55911303727566	25.18576028779164	24.889754117884877	25.365372557047817
33	24.619627565268782	24.993336964205014	25.1385718313885	25.248463639137707
34	25.236360733539083	24.62741720131896	25.205652829440446	24.930569235701512
35	25.36505067126062	24.822801874147807	25.4126253906084	24.39952206398317
36	24.760291654274063	25.343741832148147	25.028036252064894	24.86793026151289
37	25.605885617241746	24.362183312668265	25.135803613618602	24.896127456471383
38	24.908938510801843	24.68181589935533	25.679533085352524	24.729712504490305
39	25.066276283583953	24.5692846281511	25.44655215257901	24.91788693568593
40	24.516044718948642	24.285574495315274	26.180902387491262	25.01747839824482
41	24.105833471744223	24.477096538697754	26.486436376699075	24.93063361285895
42	24.898766919926405	24.094309960562555	26.19223276720061	24.814690352310432
43	24.32123944053675	23.807187838382436	26.482895633039906	25.38867708804091
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	31.0
2	35.0
3	84.5
4	134.0
5	134.0
6	198.0
7	262.0
8	286.5
9	311.0
10	409.5
11	508.0
12	508.0
13	952.5
14	1397.0
15	2616.5
16	3836.0
17	3988.0
18	4140.0
19	4140.0
20	5487.0
21	6834.0
22	8235.5
23	9637.0
24	12107.0
25	14577.0
26	14577.0
27	17528.5
28	20480.0
29	25926.0
30	31372.0
31	36255.5
32	41139.0
33	41139.0
34	46989.5
35	52840.0
36	59044.0
37	65248.0
38	73238.5
39	81229.0
40	81229.0
41	87242.5
42	93256.0
43	92243.5
44	91231.0
45	97934.0
46	104637.0
47	104637.0
48	109128.0
49	113619.0
50	117677.5
51	121736.0
52	128675.0
53	135614.0
54	135614.0
55	125762.0
56	115910.0
57	109802.0
58	103694.0
59	94892.5
60	86091.0
61	86091.0
62	79696.0
63	73301.0
64	64930.0
65	56559.0
66	49117.0
67	41675.0
68	41675.0
69	35473.0
70	29271.0
71	25606.0
72	21941.0
73	17686.5
74	13432.0
75	13432.0
76	10416.0
77	7400.0
78	6098.0
79	4796.0
80	3603.5
81	2411.0
82	2411.0
83	1788.5
84	1166.0
85	935.5
86	705.0
87	593.5
88	482.0
89	482.0
90	400.5
91	319.0
92	191.0
93	63.0
94	40.5
95	18.0
96	18.0
97	12.5
98	7.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1553346.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.75033735563788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.84616108814589	50.43490924615005
2	8.471626140644963	9.954217874274644
3	2.2466874119086966	3.959809301469027
4	0.9669573062901103	2.2723627181217143
5	0.5246585587573357	1.5411933661758117
6	0.32681378582369386	1.1520252101769122
7	0.24215867913541145	0.9958832864960765
8	0.1713166829328685	0.8051930333563901
9	0.12845764966628914	0.6792237228426149
>10	0.862289654745633	9.820419841348908
>50	0.11141682666394233	4.5540991109199815
>100	0.09409963498228441	10.682945671746518
>500	0.006148783536812563	2.2704129282148235
>1k	0.0012077967661596107	0.8773046887067167
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1805	0.11620076917827708	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1568	0.10094338286511827	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.437715743948869E-5	0.0	0.0	0.0
3	0.0	6.437715743948869E-5	0.0	0.0	0.0
4	0.0	6.437715743948869E-5	0.0	0.0	0.0
5	0.0	1.2875431487897738E-4	0.0	0.0	0.0
6	0.0	1.2875431487897738E-4	0.0	0.0	0.0
7	0.0	1.2875431487897738E-4	0.0	0.0	0.0
8	0.0	1.2875431487897738E-4	0.0	6.437715743948869E-5	0.0
9	0.0	1.2875431487897738E-4	0.0	6.437715743948869E-5	0.0
10	0.0	1.2875431487897738E-4	0.0	6.437715743948869E-5	0.0
11	0.0	3.2188578719744346E-4	0.0	6.437715743948869E-5	0.0
12	0.0	3.2188578719744346E-4	0.0	6.437715743948869E-5	0.0
13	0.0	3.8626294463693216E-4	0.0	6.437715743948869E-5	0.0
14	0.0	3.8626294463693216E-4	0.0	6.437715743948869E-5	0.0
15	0.0	3.8626294463693216E-4	0.0	1.2875431487897738E-4	0.0
16	0.0	3.8626294463693216E-4	0.0	2.5750862975795476E-4	0.0
17	0.0	3.8626294463693216E-4	0.0	4.506401020764208E-4	0.0
18	0.0	3.8626294463693216E-4	0.0	5.150172595159095E-4	0.0
19	0.0	3.8626294463693216E-4	0.0	5.150172595159095E-4	0.0
20	0.0	3.8626294463693216E-4	0.0	6.437715743948869E-4	0.0
21	0.0	3.8626294463693216E-4	0.0	9.012802041528416E-4	0.0
22	0.0	3.8626294463693216E-4	0.0	0.0013519203062292626	0.0
23	0.0	3.8626294463693216E-4	0.0	0.001866937565745172	0.0
24	0.0	3.8626294463693216E-4	0.0	0.0028969720847769912	0.0
25	0.0	3.8626294463693216E-4	0.0	0.003154480714534946	0.0
26	0.0	3.8626294463693216E-4	0.0	0.004055760918687788	0.0
27	0.0	3.8626294463693216E-4	0.0	0.007467750262980688	0.0
28	0.0	3.8626294463693216E-4	0.0	0.01609428935987217	0.0
29	0.0	3.8626294463693216E-4	0.0	0.031673561460228435	0.0
30	0.0	3.8626294463693216E-4	0.0	0.05362617214709408	0.0
31	0.0	3.8626294463693216E-4	0.0	0.14471984992397058	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	45	0.0038264047	20.555555	5
ATATCGC	55	5.1442115E-4	20.181818	6
GGTATCA	780	0.0	19.923077	1
TATTTCG	80	1.6172664E-5	18.5	29
GCTCGTT	80	1.6172664E-5	18.5	11
TGTCGCG	50	0.007035968	18.5	22
ATGCGTT	95	3.6080364E-6	17.526316	36
TACGTTA	75	2.0678557E-4	17.266666	19
GTGTAGA	140	1.869921E-9	17.178572	1
TCGTTTA	165	5.4569682E-11	16.818182	30
GTGTTAC	135	2.2208042E-8	16.444445	1
GTATGTA	205	0.0	16.243904	1
ACCGCGA	80	3.3835223E-4	16.1875	36
AATTACG	80	3.3835223E-4	16.1875	16
CGCAAGA	355	0.0	16.15493	2
AAGACGG	345	0.0	16.086956	5
GCGCAAG	380	0.0	16.06579	1
CGAACGA	140	3.475361E-8	15.857142	16
CGGACCA	330	0.0	15.69697	9
GACGGTA	190	2.7284841E-11	15.578948	8
>>END_MODULE