FastQCFastQC Report
Fri 10 Feb 2017
ERR1630392.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630392.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334145
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG6240.18674527525475468No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT4180.12509539271872988No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG4000.1197085097786889No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA3800.11372308428975444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACT200.001840068337.04
TAGACCT303.5940637E-430.8333324
AGTATAC250.00549083329.63
CAAGACG358.8561774E-426.4285724
AAGACGG509.065909E-625.9000025
GCTTTAG551.8974266E-523.54545413
GTCGTTG400.001928336923.1251
GGTATCA2300.022.521741
TGCTTTA603.7161124E-521.58333212
TCTTATA1151.3460522E-1020.91304437
GTGTTAG450.00382021620.5555531
TCGTTGG450.00382021620.5555532
TATGGTG450.00382021620.5555535
ATATTCT450.00382021620.5555534
TAGACAG555.132606E-420.1818185
GCTTAGG1650.020.1818181
ATGTAAA656.883417E-519.9230771
ATGTGTA500.007024695618.57
ATTCGTA500.007024695618.530
GCGCAAG500.007024695618.51
Produced by FastQC (version 0.11.5)