##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630392.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	334145
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.696326445106166	31.0	31.0	34.0	30.0	34.0
2	31.89894806147032	33.0	31.0	34.0	30.0	34.0
3	32.03160603929432	33.0	31.0	34.0	30.0	34.0
4	35.70817459486151	37.0	35.0	37.0	33.0	37.0
5	35.606494186655496	37.0	35.0	37.0	33.0	37.0
6	35.686094360232836	37.0	35.0	37.0	33.0	37.0
7	35.64616558679615	37.0	35.0	37.0	33.0	37.0
8	35.625674482634786	37.0	35.0	37.0	33.0	37.0
9	37.333301411064056	39.0	37.0	39.0	34.0	39.0
10	37.22426790764489	39.0	37.0	39.0	34.0	39.0
11	37.32272516422511	39.0	37.0	39.0	34.0	39.0
12	37.18636220802346	39.0	37.0	39.0	33.0	39.0
13	37.20784988552874	39.0	37.0	39.0	34.0	39.0
14	38.392150114471264	40.0	38.0	41.0	34.0	41.0
15	38.3998922623412	40.0	38.0	41.0	34.0	41.0
16	38.36209430037858	40.0	38.0	41.0	34.0	41.0
17	38.325972257552856	40.0	38.0	41.0	33.0	41.0
18	38.283122596477575	40.0	38.0	41.0	33.0	41.0
19	38.30853372039085	40.0	38.0	41.0	34.0	41.0
20	38.24852683715154	40.0	38.0	41.0	34.0	41.0
21	38.18843615795538	40.0	38.0	41.0	33.0	41.0
22	38.16093312783372	40.0	38.0	41.0	33.0	41.0
23	38.1013093118257	40.0	38.0	41.0	33.0	41.0
24	38.07423723233926	40.0	37.0	41.0	33.0	41.0
25	38.02187074473657	40.0	37.0	41.0	33.0	41.0
26	37.867761001960226	40.0	37.0	41.0	33.0	41.0
27	37.76068173996319	40.0	37.0	41.0	33.0	41.0
28	37.6713193374134	40.0	37.0	41.0	32.0	41.0
29	37.600846937706685	40.0	37.0	41.0	32.0	41.0
30	37.56113064687486	40.0	37.0	41.0	32.0	41.0
31	37.449765820227746	40.0	37.0	41.0	32.0	41.0
32	37.3377934728935	39.0	37.0	41.0	31.0	41.0
33	37.288030645378505	39.0	36.0	41.0	31.0	41.0
34	37.1971599156055	39.0	36.0	41.0	31.0	41.0
35	37.089565907016414	39.0	36.0	41.0	31.0	41.0
36	37.0082509090365	39.0	36.0	41.0	30.0	41.0
37	36.95820676652352	39.0	36.0	41.0	30.0	41.0
38	36.837331098774484	39.0	36.0	40.0	30.0	41.0
39	36.72827963907884	39.0	35.0	40.0	30.0	41.0
40	36.64913136512592	39.0	35.0	40.0	30.0	41.0
41	36.525559263194125	39.0	35.0	40.0	30.0	41.0
42	36.49212766912568	39.0	35.0	40.0	30.0	41.0
43	35.177638450373344	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	1.0
17	4.0
18	20.0
19	55.0
20	75.0
21	164.0
22	262.0
23	439.0
24	701.0
25	1024.0
26	1544.0
27	2257.0
28	3167.0
29	4411.0
30	5768.0
31	7698.0
32	10001.0
33	12545.0
34	16695.0
35	22050.0
36	30758.0
37	45260.0
38	82294.0
39	86951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.55128761465831	18.227715512726512	12.136647263912373	27.08434960870281
2	20.574600846937706	20.749375271214593	32.021128551975934	26.654895329871763
3	21.01692379056996	21.364377740202606	28.898831345673287	28.719867123554145
4	16.491642849661076	14.987804695566295	31.907405467686186	36.613146987086445
5	16.055305331517754	35.42294512861183	33.079651049694	15.44209849017642
6	37.81531969653893	32.678926813209834	14.316239955707852	15.189513534543387
7	30.780349848119826	29.056547307306708	19.79918897484625	20.363913869727217
8	28.121923117209597	32.31860419877598	19.548698918134345	20.01077376588008
9	27.322270271887955	13.504017716859448	18.131350162354668	41.04236184889793
10	17.78210058507534	26.145535620763443	31.791886755749747	24.28047703841147
11	37.686633048526836	20.427060108635473	19.38170554699307	22.504601295844616
12	22.859237756063987	24.485178590133025	27.191488724954738	25.46409492884825
13	32.11480046087776	18.29714644839815	23.367400380074518	26.220652710649567
14	24.59351479148274	19.58401292851906	22.460009875952057	33.36246240404615
15	27.442577324215534	26.072214158523995	20.282811354352155	26.20239716290832
16	27.215131155636023	24.656361759116553	21.538852893205046	26.589654192042378
17	25.780723937212883	24.63720839755196	23.197115024914332	26.38495264032082
18	25.09868470274881	23.534992293764684	24.75242783821395	26.61389516527256
19	26.73450148887459	23.678043962950216	23.985096290532553	25.60235825764264
20	27.13372936898652	22.79579224588128	24.17453500725733	25.895943377874875
21	27.081955438507233	23.464364272995255	22.841880022146075	26.61180026635143
22	27.07626928429275	23.244399886276916	23.209684418441096	26.469646410989238
23	26.13625821125559	23.772015143126488	23.21387421628335	26.87785242933457
24	26.86947283365006	24.200571608134194	23.324006045279745	25.605949512936
25	27.08255398105613	23.698992952161486	23.130377530712714	26.08807553606967
26	27.11517454997082	23.958461147106792	22.605455715333164	26.32090858758922
27	27.1810142303491	23.06334076523665	23.148034535905072	26.60761046850918
28	26.267039758188808	23.35273608762663	23.475138038875336	26.905086115309224
29	25.473372338356103	23.804037169492286	23.913869727214234	26.808720764937377
30	25.76456328839276	24.948151251702104	23.45748103368298	25.829804426222147
31	27.14091187957324	24.054227954929747	22.840383665773842	25.964476499723173
32	25.373415732690894	24.062607550614253	23.68313157461581	26.880845142079036
33	25.818432117793172	23.57359828816831	23.794460488709994	26.813509105328524
34	26.28439749210672	23.26834158823265	24.156578730790525	26.2906821888701
35	26.326894013078157	23.368597465172307	24.087447066393334	26.217061455356205
36	25.970162653937663	24.316389591345075	23.696299510691464	26.017148244025794
37	27.121159975459758	23.259363449999253	23.31532717832079	26.304149396220204
38	25.875293659938052	23.56342306483712	24.59800386059944	25.96327941462539
39	26.118601206063236	23.200706280207694	24.33614149545856	26.34455101827051
40	25.057684538149605	22.70810576246839	25.714584985560162	26.519624713821845
41	24.66833261009442	23.013661733678493	26.28260186446004	26.03540379176705
42	25.533525864519895	22.280147840009576	25.838184021906656	26.348142273563873
43	24.653369046372084	22.16762184081761	26.100046387047538	27.078962725762768
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	7.0
2	7.0
3	12.5
4	18.0
5	18.0
6	29.0
7	40.0
8	44.5
9	49.0
10	51.0
11	53.0
12	53.0
13	116.0
14	179.0
15	270.0
16	361.0
17	381.5
18	402.0
19	402.0
20	507.5
21	613.0
22	698.0
23	783.0
24	1035.0
25	1287.0
26	1287.0
27	1664.0
28	2041.0
29	3080.0
30	4119.0
31	4811.5
32	5504.0
33	5504.0
34	6377.0
35	7250.0
36	8329.5
37	9409.0
38	11498.0
39	13587.0
40	13587.0
41	15097.0
42	16607.0
43	16591.0
44	16575.0
45	18677.5
46	20780.0
47	20780.0
48	22716.5
49	24653.0
50	26618.0
51	28583.0
52	31554.0
53	34525.0
54	34525.0
55	32026.0
56	29527.0
57	28722.5
58	27918.0
59	25764.0
60	23610.0
61	23610.0
62	21842.5
63	20075.0
64	17716.5
65	15358.0
66	13153.0
67	10948.0
68	10948.0
69	9017.0
70	7086.0
71	6212.0
72	5338.0
73	4157.5
74	2977.0
75	2977.0
76	2302.0
77	1627.0
78	1393.5
79	1160.0
80	836.5
81	513.0
82	513.0
83	394.0
84	275.0
85	211.0
86	147.0
87	115.5
88	84.0
89	84.0
90	68.5
91	53.0
92	33.0
93	13.0
94	8.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	334145.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.648402591191
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.77878677395286	59.83612725059103
2	6.1488282243492085	8.196191579210083
3	1.4391286285491767	2.8774687264816228
4	0.6333042750050516	1.6883487313303607
5	0.3713806722797101	1.2375964280342644
6	0.2333899685007592	0.9333041148830389
7	0.17782468317286332	0.8296211752329187
8	0.1382134444781295	0.736936423287488
9	0.10612805828530653	0.6365938999338444
>10	0.7890015181832633	11.026565082697433
>50	0.12912456523454058	6.032858109346641
>100	0.05443927663197636	5.7812766215115605
>500	4.4991137712377155E-4	0.18711185745971026
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	624	0.18674527525475468	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	418	0.12509539271872988	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	400	0.1197085097786889	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	380	0.11372308428975444	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	2.992712744467222E-4	0.0
18	0.0	0.0	0.0	8.978138233401667E-4	0.0
19	0.0	0.0	0.0	0.0014963563722336112	0.0
20	0.0	0.0	0.0	0.0014963563722336112	0.0
21	0.0	0.0	0.0	0.0014963563722336112	0.0
22	0.0	0.0	0.0	0.002394170195573778	0.0
23	0.0	0.0	0.0	0.0032919840189139446	0.0
24	0.0	0.0	0.0	0.004788340391147556	0.0
25	0.0	0.0	0.0	0.005087611665594278	0.0
26	0.0	0.0	0.0	0.006284696763381167	0.0
27	0.0	0.0	0.0	0.010175223331188556	0.0
28	0.0	0.0	0.0	0.021846803034610722	0.0
29	0.0	0.0	0.0	0.044591419892561615	0.0
30	0.0	0.0	0.0	0.075416361160574	0.0
31	0.0	0.0	0.0	0.16938754133684478	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACT	20	0.0018400683	37.0	4
TAGACCT	30	3.5940637E-4	30.833332	4
AGTATAC	25	0.005490833	29.6	3
CAAGACG	35	8.8561774E-4	26.428572	4
AAGACGG	50	9.065909E-6	25.900002	5
GCTTTAG	55	1.8974266E-5	23.545454	13
GTCGTTG	40	0.0019283369	23.125	1
GGTATCA	230	0.0	22.52174	1
TGCTTTA	60	3.7161124E-5	21.583332	12
TCTTATA	115	1.3460522E-10	20.913044	37
GTGTTAG	45	0.003820216	20.555553	1
TCGTTGG	45	0.003820216	20.555553	2
TATGGTG	45	0.003820216	20.555553	5
ATATTCT	45	0.003820216	20.555553	4
TAGACAG	55	5.132606E-4	20.181818	5
GCTTAGG	165	0.0	20.181818	1
ATGTAAA	65	6.883417E-5	19.923077	1
ATGTGTA	50	0.0070246956	18.5	7
ATTCGTA	50	0.0070246956	18.5	30
GCGCAAG	50	0.0070246956	18.5	1
>>END_MODULE