FastQCFastQC Report
Fri 10 Feb 2017
ERR1630391.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630391.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402787
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40870.29134857964894173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38940.27759025425813044No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG30330.21621243994989972No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30210.21535700002922753No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG19820.1412901602310258No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17830.1271041148798784No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT17800.12689025489971034No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA17520.12489422841814189No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA17510.12482294175808585No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC16570.11812199571282027No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT14850.10586069018318532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG1500.022.228
CCGCTTA450.003826221520.55555725
AGACGTA450.003826221520.5555575
TCGCCAT3900.019.92307713
CTAGCGG1600.019.6562529
CGCAATA1650.019.06060836
TAGCGAG500.00703563518.528
CGTAAAT609.238197E-418.517
ATACCGT1451.546141E-1017.8620686
TACCGTC1451.546141E-1017.8620687
GGTATCA18900.017.423281
CTAGTAC852.7238078E-517.4117643
TAACCCG2250.017.26666628
CGGTCCA2100.016.73809610
GTAACCC2550.015.96078427
AATACGA700.00259287615.8571422
ATTGCCG2700.015.75925811
CGAACGA1902.7284841E-1115.57894816
ACGCTTT957.0614464E-515.57894814
ACCCGTT2850.015.57894730
Produced by FastQC (version 0.11.5)