##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630391.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1402787
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8190388134478	31.0	31.0	34.0	30.0	34.0
2	32.01698689822475	33.0	31.0	34.0	30.0	34.0
3	32.15126530257267	34.0	31.0	34.0	30.0	34.0
4	35.80823888444932	37.0	35.0	37.0	35.0	37.0
5	35.73010870502792	37.0	35.0	37.0	35.0	37.0
6	35.79749242044587	37.0	35.0	37.0	35.0	37.0
7	35.7757649593274	37.0	35.0	37.0	35.0	37.0
8	35.75276788279332	37.0	35.0	37.0	33.0	37.0
9	37.45945606852644	39.0	37.0	39.0	35.0	39.0
10	37.366859687179875	39.0	37.0	39.0	34.0	39.0
11	37.45016670385454	39.0	37.0	39.0	35.0	39.0
12	37.33080075592375	39.0	37.0	39.0	34.0	39.0
13	37.364917838559954	39.0	37.0	39.0	34.0	39.0
14	38.577099730750284	40.0	38.0	41.0	34.0	41.0
15	38.58016648286589	40.0	38.0	41.0	34.0	41.0
16	38.558757673117874	40.0	38.0	41.0	34.0	41.0
17	38.53878600243658	40.0	38.0	41.0	34.0	41.0
18	38.50316690987299	40.0	38.0	41.0	34.0	41.0
19	38.53071563965164	40.0	38.0	41.0	34.0	41.0
20	38.489159081171984	40.0	38.0	41.0	34.0	41.0
21	38.447281019855474	40.0	38.0	41.0	34.0	41.0
22	38.426796798088375	40.0	38.0	41.0	34.0	41.0
23	38.363517768556456	40.0	38.0	41.0	34.0	41.0
24	38.35595639252431	40.0	38.0	41.0	34.0	41.0
25	38.328689245052885	40.0	38.0	41.0	34.0	41.0
26	38.17369707589249	40.0	38.0	41.0	34.0	41.0
27	38.07399626600475	40.0	38.0	41.0	33.0	41.0
28	37.995479712885846	40.0	37.0	41.0	33.0	41.0
29	37.928853061797696	40.0	37.0	41.0	33.0	41.0
30	37.86488754172943	40.0	37.0	41.0	33.0	41.0
31	37.763008211510375	40.0	37.0	41.0	33.0	41.0
32	37.66319904589934	40.0	37.0	41.0	33.0	41.0
33	37.60123169091245	40.0	37.0	41.0	32.0	41.0
34	37.51444160802745	40.0	37.0	41.0	32.0	41.0
35	37.421418932453754	40.0	37.0	41.0	32.0	41.0
36	37.32895157996189	40.0	36.0	41.0	31.0	41.0
37	37.27307780867658	39.0	36.0	41.0	31.0	41.0
38	37.166959060783995	39.0	36.0	41.0	31.0	41.0
39	37.054502928812425	39.0	36.0	41.0	31.0	41.0
40	36.96361029864121	39.0	36.0	41.0	31.0	41.0
41	36.82816208020177	39.0	36.0	41.0	30.0	41.0
42	36.771880549220946	39.0	35.0	41.0	30.0	41.0
43	35.60232736687751	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	3.0
12	1.0
13	6.0
14	4.0
15	3.0
16	14.0
17	16.0
18	55.0
19	128.0
20	240.0
21	448.0
22	800.0
23	1395.0
24	2231.0
25	3490.0
26	5260.0
27	7727.0
28	11253.0
29	15557.0
30	21431.0
31	28298.0
32	37061.0
33	48490.0
34	64237.0
35	86174.0
36	121612.0
37	186955.0
38	340214.0
39	419679.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.60468624245876	19.34035601983765	12.705706568424144	25.34925116927944
2	18.26920266583594	21.317705396471453	35.127214609202966	25.285877328489644
3	19.826958761380023	23.244369957805425	30.586895943575183	26.341775337239365
4	14.65311554783442	15.634162563525326	34.54865207618833	35.16406981245193
5	13.855061388507306	37.68440967873241	34.377492805393835	14.08303612736645
6	34.29572700630958	35.05749625566818	16.01868280786748	14.628093930154757
7	28.88827740776041	31.452244709995174	20.986792720491422	18.672685161752998
8	27.167987727288605	34.348051414790696	19.926760085458447	18.55720077246225
9	26.677749366083376	14.490368102926531	19.638833265492195	39.1930492654979
10	17.03480286030595	27.473950072249032	32.676307949817044	22.81493911762798
11	35.40159696375858	22.17186215726265	21.20015369403908	21.226387184939696
12	21.01815885091607	26.36266232863578	28.990217331640512	23.628961488807636
13	29.908888519782405	19.67690034196211	25.777541422895993	24.636669715359496
14	22.696104255314598	21.22809806478104	24.981340716730337	31.09445696317402
15	25.46010192566655	28.104195433804275	22.29083959289614	24.144863047633034
16	25.010782107333473	26.943220888131986	23.745158744698948	24.3008382598356
17	23.70587979500808	27.132344397260592	25.133395162629824	24.028380645101503
18	22.84659039469285	25.450264366578818	27.06875669649063	24.634388542237705
19	24.130962148922112	25.90036833817251	27.02869359353915	22.939975919366233
20	24.46779161768679	24.782736081814274	27.08101800202026	23.66845429847867
21	24.434215600800407	25.761430637723333	25.831505424558397	23.972848336917867
22	24.57700278089261	25.26527548373345	26.106814505694736	24.050907229679204
23	23.638727761235312	25.796218527830668	26.463390379294932	24.101663331639088
24	24.25892170372266	26.14217268908252	26.23791067353775	23.36099493365707
25	24.434215600800407	25.468086031592822	26.238979973438592	23.85871839416818
26	24.324434144314143	26.074878081989638	25.943425480846344	23.657262292849875
27	24.61820647040499	25.267200223554966	25.972724298129368	24.141869007910678
28	23.637444601354304	25.62042562413253	26.811697000328632	23.930432774184535
29	23.53365122431274	25.94278390090584	26.50580594202826	24.017758932753157
30	22.977472702555698	27.10026540023539	26.50024558254389	23.42201631466502
31	24.22406252695527	26.053135650672555	26.201198043608905	23.521603778763275
32	23.156616079276468	26.174465546087895	26.236627513656742	24.432290860978895
33	23.280583581113884	25.72279326797297	26.76286563819026	24.233757512722885
34	23.92458726805994	25.382542039525603	26.647880255519908	24.044990436894555
35	24.09339407907259	25.52425991971696	26.836148324727844	23.546197676482603
36	23.386016551336734	26.35318120284833	26.431382668929782	23.829419576885158
37	24.694055476704587	25.09247661975767	26.162132953898205	24.05133494963954
38	23.672660211421974	25.49139676943114	27.036392552825195	23.799550466321687
39	23.886021184969636	25.31717217225423	26.859245202585996	23.937561440190137
40	23.279300421232875	24.836272363516343	27.809282521152532	24.07514469409825
41	22.687763716088043	25.577225908138583	27.96240626695286	23.772604108820513
42	23.614918016776603	24.66953286564532	27.71603956979926	23.999509547778814
43	22.889291104066405	24.634032108937422	27.753037346368338	24.72363944062784
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	86.0
1	96.5
2	107.0
3	344.0
4	581.0
5	581.0
6	799.0
7	1017.0
8	982.5
9	948.0
10	1397.0
11	1846.0
12	1846.0
13	3329.0
14	4812.0
15	7435.0
16	10058.0
17	9633.5
18	9209.0
19	9209.0
20	10577.5
21	11946.0
22	12137.5
23	12329.0
24	14276.0
25	16223.0
26	16223.0
27	19062.5
28	21902.0
29	29899.5
30	37897.0
31	39768.5
32	41640.0
33	41640.0
34	46276.0
35	50912.0
36	55468.5
37	60025.0
38	67976.5
39	75928.0
40	75928.0
41	83660.5
42	91393.0
43	87947.5
44	84502.0
45	91015.5
46	97529.0
47	97529.0
48	102336.5
49	107144.0
50	107603.5
51	108063.0
52	114403.0
53	120743.0
54	120743.0
55	107195.0
56	93647.0
57	88420.0
58	83193.0
59	74908.5
60	66624.0
61	66624.0
62	62184.0
63	57744.0
64	51393.5
65	45043.0
66	38882.5
67	32722.0
68	32722.0
69	26997.0
70	21272.0
71	18390.0
72	15508.0
73	12113.0
74	8718.0
75	8718.0
76	6721.5
77	4725.0
78	3969.5
79	3214.0
80	2424.5
81	1635.0
82	1635.0
83	1213.5
84	792.0
85	648.0
86	504.0
87	406.0
88	308.0
89	308.0
90	272.5
91	237.0
92	135.0
93	33.0
94	27.5
95	22.0
96	22.0
97	12.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1402787.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.67993360066545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.60215456768105	37.95425444256941
2	11.82817223034387	11.279329331200612
3	3.4374023155621583	4.91685342494332
4	1.505559943479184	2.8713999254763647
5	0.821688077811643	1.958901649525878
6	0.5071689011165831	1.4509067717736681
7	0.36152534981899453	1.2066253272028902
8	0.24316756512659427	0.9275370687257204
9	0.17605050414573245	0.7554668713228979
>10	1.2102548134434885	11.099037804385745
>50	0.14958980223796847	5.021062472935898
>100	0.14167427533133495	13.84464069156871
>500	0.011993579924299662	3.7659385533350602
>1k	0.003598073977289898	2.9480456650338724
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4087	0.29134857964894173	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3894	0.27759025425813044	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3033	0.21621243994989972	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3021	0.21535700002922753	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1982	0.1412901602310258	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1783	0.1271041148798784	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1780	0.12689025489971034	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1752	0.12489422841814189	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1751	0.12482294175808585	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1657	0.11812199571282027	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	1485	0.10586069018318532	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	7.128666005601705E-5	0.0
13	0.0	0.0	0.0	1.425733201120341E-4	0.0
14	0.0	0.0	0.0	1.425733201120341E-4	0.0
15	0.0	0.0	0.0	3.5643330028008527E-4	0.0
16	0.0	0.0	0.0	4.277199603361023E-4	0.0
17	0.0	0.0	0.0	7.128666005601705E-4	0.0
18	0.0	0.0	0.0	9.980132407842388E-4	0.0
19	0.0	0.0	0.0	0.00121187322095229	0.0
20	0.0	0.0	0.0	0.001283159881008307	0.0
21	0.0	0.0	0.0	0.0016395931812883922	0.0
22	0.0	0.0	0.0	0.002566319762016614	0.0
23	0.0	0.0	0.0	0.004918779543865177	0.0
24	0.0	0.0	0.0	0.007698959286049842	0.0
25	0.0	0.0	0.0	0.008625685866778064	0.0
26	0.0	0.0	0.0	0.01090685898857061	0.0
27	0.0	0.0	0.0	0.01739394505366816	0.0
28	0.0	0.0	0.0	0.03806707646991311	0.0
29	0.0	0.0	0.0	0.0685064803138324	0.0
30	0.0	0.0	0.0	0.11712398247203602	0.0
31	0.0	0.0	0.0	0.25506366968042904	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	150	0.0	22.2	28
CCGCTTA	45	0.0038262215	20.555557	25
AGACGTA	45	0.0038262215	20.555557	5
TCGCCAT	390	0.0	19.923077	13
CTAGCGG	160	0.0	19.65625	29
CGCAATA	165	0.0	19.060608	36
TAGCGAG	50	0.007035635	18.5	28
CGTAAAT	60	9.238197E-4	18.5	17
ATACCGT	145	1.546141E-10	17.862068	6
TACCGTC	145	1.546141E-10	17.862068	7
GGTATCA	1890	0.0	17.42328	1
CTAGTAC	85	2.7238078E-5	17.411764	3
TAACCCG	225	0.0	17.266666	28
CGGTCCA	210	0.0	16.738096	10
GTAACCC	255	0.0	15.960784	27
AATACGA	70	0.002592876	15.857142	2
ATTGCCG	270	0.0	15.759258	11
CGAACGA	190	2.7284841E-11	15.578948	16
ACGCTTT	95	7.0614464E-5	15.578948	14
ACCCGTT	285	0.0	15.578947	30
>>END_MODULE