FastQCFastQC Report
Fri 10 Feb 2017
ERR1630390.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630390.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267919
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC3010.11234738857639809No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2960.11048115288576024No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGC200.00183941837.022
CGCATCG250.00548891629.59999813
CCGGTCC358.851577E-426.428579
CCTATAC551.8956061E-523.5454543
TGGCGCA400.001927344723.12514
GAAGTGT400.001927344723.1256
GTAGGAC502.694614E-422.1999993
CCTACAC450.003818267720.5555553
CTGACCA450.003818267720.5555559
GGTATCA2150.019.7906971
TTAGAGC609.211633E-418.53
GGATAAT609.211633E-418.51
GTCTTGC609.211633E-418.51
TATACAC801.6091424E-518.537
GGCTACA609.211633E-418.51
CATACAC500.007021151518.4999983
TGAATTA500.007021151518.49999821
ACGGAAT752.05982E-417.26666618
CGGACAT904.425514E-516.44444521
ACCCACG803.3704692E-416.187514
Produced by FastQC (version 0.11.5)