##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630390.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	267919
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.823969184716276	31.0	31.0	34.0	30.0	34.0
2	32.025731657702515	33.0	31.0	34.0	30.0	34.0
3	32.17523579887951	34.0	31.0	34.0	30.0	34.0
4	35.81151392771696	37.0	35.0	37.0	35.0	37.0
5	35.728242491200696	37.0	35.0	37.0	35.0	37.0
6	35.80226486363416	37.0	35.0	37.0	35.0	37.0
7	35.77321503887369	37.0	35.0	37.0	35.0	37.0
8	35.753328431354255	37.0	35.0	37.0	33.0	37.0
9	37.469679268734204	39.0	37.0	39.0	35.0	39.0
10	37.37350467865288	39.0	37.0	39.0	34.0	39.0
11	37.476894135914215	39.0	37.0	39.0	35.0	39.0
12	37.352793194958174	39.0	37.0	39.0	34.0	39.0
13	37.38566880288445	39.0	37.0	39.0	34.0	39.0
14	38.587289441958205	40.0	38.0	41.0	34.0	41.0
15	38.59249623953508	40.0	38.0	41.0	34.0	41.0
16	38.56560751570437	40.0	38.0	41.0	34.0	41.0
17	38.54137257902575	40.0	38.0	41.0	34.0	41.0
18	38.52042221716265	40.0	38.0	41.0	34.0	41.0
19	38.530660386161486	40.0	38.0	41.0	34.0	41.0
20	38.49192853063799	40.0	38.0	41.0	34.0	41.0
21	38.44013675775141	40.0	38.0	41.0	34.0	41.0
22	38.406902832572534	40.0	38.0	41.0	34.0	41.0
23	38.35181528745628	40.0	38.0	41.0	34.0	41.0
24	38.35308059525454	40.0	38.0	41.0	34.0	41.0
25	38.30373359112269	40.0	38.0	41.0	34.0	41.0
26	38.16456093072907	40.0	38.0	41.0	33.0	41.0
27	38.063097428700466	40.0	38.0	41.0	33.0	41.0
28	37.98557773058275	40.0	37.0	41.0	33.0	41.0
29	37.90951369630373	40.0	37.0	41.0	33.0	41.0
30	37.84940970965105	40.0	37.0	41.0	33.0	41.0
31	37.76945270772136	40.0	37.0	41.0	33.0	41.0
32	37.68269514293499	40.0	37.0	41.0	32.0	41.0
33	37.62615193398005	40.0	37.0	41.0	32.0	41.0
34	37.54768792060287	40.0	37.0	41.0	32.0	41.0
35	37.46782049798633	40.0	37.0	41.0	32.0	41.0
36	37.381417517981184	40.0	37.0	41.0	31.0	41.0
37	37.33019308074455	39.0	37.0	41.0	31.0	41.0
38	37.239426841694694	39.0	36.0	41.0	31.0	41.0
39	37.135548430682405	39.0	36.0	41.0	31.0	41.0
40	37.04805556903393	39.0	36.0	41.0	31.0	41.0
41	36.94593141957084	39.0	36.0	41.0	31.0	41.0
42	36.90189198974317	39.0	36.0	41.0	30.0	41.0
43	35.697737002601535	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	0.0
15	0.0
16	2.0
17	6.0
18	6.0
19	14.0
20	35.0
21	87.0
22	124.0
23	252.0
24	422.0
25	674.0
26	970.0
27	1465.0
28	2089.0
29	2960.0
30	4026.0
31	5337.0
32	7077.0
33	9067.0
34	12424.0
35	16615.0
36	23125.0
37	35101.0
38	65050.0
39	80989.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.39564943135798	19.34390618059936	12.22757624505914	26.03286814298351
2	20.108316319484622	20.675278722300398	32.855452580817335	26.360952377397645
3	20.583086679182887	22.537035447280708	29.025190449352227	27.854687424184178
4	15.394578212071558	15.393085223519048	34.455189814832096	34.7571467495773
5	15.065000989104915	36.25386777346885	34.37083596161526	14.310295275810972
6	35.55664211944654	34.69033551185246	15.051564092132324	14.701458276568665
7	29.883285619907507	29.592899346444263	20.99552476681385	19.52829026683438
8	28.004732773711456	32.36164661707456	19.820542775988265	19.813077833225716
9	27.20038519104655	13.50333496317917	19.225213590674795	40.07106625509949
10	17.909890675913243	25.776074111951747	32.966306980841225	23.34772823129379
11	36.919740667888426	21.178789111634487	19.90415013492884	21.997320085548246
12	22.187676125993306	25.61856381966191	27.85394092990792	24.33981912443686
13	31.690548262721197	19.00611752059391	23.852731609180385	25.450602607504507
14	23.75979307178662	20.487162164684104	23.66162907445907	32.0914156890702
15	26.879765899394965	26.900667739130107	21.083984338550085	25.13558202292484
16	26.16686386557131	25.4356727219794	22.925585718071506	25.471877694377774
17	24.65782568612155	25.364755765735165	24.457391972947047	25.520026575196237
18	24.625353185104455	24.088997047615138	25.65140956781714	25.63424019946327
19	25.035925037044777	24.501061888107973	25.237851738771795	25.22516133607546
20	26.24002030464431	23.86280928190983	24.925817131297144	24.97135328214871
21	26.32997286493306	24.464110421433343	24.04532713245421	25.160589581179387
22	26.429629850813118	24.26964866246888	24.42342648337744	24.877295003340564
23	25.920147507268986	23.97441017620997	24.624233443690073	25.48120887283097
24	25.52039982233436	24.684699480066737	24.270395156745135	25.524505540853763
25	26.0448120514036	24.2577047540488	24.39095398236034	25.306529212187268
26	25.518160339505595	24.9116337400483	24.06398948936059	25.506216431085516
27	25.794363221719998	24.2618104725682	24.408869844990463	25.534956460721336
28	25.4640395044771	24.361094211310135	24.88961215889877	25.285254125313994
29	24.695523647072438	24.452913007289517	25.393869042509117	25.457694303128932
30	24.953810666656715	25.787271526095573	24.67536830161355	24.583549505634167
31	26.004501360485822	25.157976851212492	24.04868635669736	24.788835431604326
32	25.07213000944315	24.803765317129432	24.283832053717727	25.840272619709687
33	24.707467555492517	24.548091027512047	25.116173171742208	25.628268245253228
34	25.531597236478188	23.73889123205148	25.06354532526622	25.665966206204118
35	25.165441793975045	24.265169696811352	25.776820606228	24.792567902985603
36	24.73919356223336	25.096391073421444	24.75636293058723	25.408052433757966
37	26.12282070327226	23.788533101422445	24.92992284981655	25.15872334548875
38	24.996360840403256	24.428651943311223	25.293838809490925	25.28114840679459
39	24.777264770322375	24.25023981128625	25.77420787626111	25.19828754213027
40	24.286444783684622	23.707538472448764	26.686423881844885	25.31959286202173
41	23.987847073182568	23.640727234723926	26.985768086623196	25.385657605470307
42	24.180069349318263	23.45298392424576	27.055192054314926	25.31175467212105
43	23.716869650901952	22.774047379991714	27.32169051093801	26.18739245816833
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	4.5
2	3.0
3	16.5
4	30.0
5	30.0
6	33.5
7	37.0
8	40.5
9	44.0
10	55.0
11	66.0
12	66.0
13	136.5
14	207.0
15	313.0
16	419.0
17	444.5
18	470.0
19	470.0
20	677.5
21	885.0
22	1066.5
23	1248.0
24	1622.5
25	1997.0
26	1997.0
27	2441.0
28	2885.0
29	3685.0
30	4485.0
31	5430.5
32	6376.0
33	6376.0
34	7286.5
35	8197.0
36	9547.0
37	10897.0
38	11646.0
39	12395.0
40	12395.0
41	13968.5
42	15542.0
43	16261.0
44	16980.0
45	17856.0
46	18732.0
47	18732.0
48	19496.0
49	20260.0
50	20280.0
51	20300.0
52	21123.0
53	21946.0
54	21946.0
55	21415.0
56	20884.0
57	20162.0
58	19440.0
59	18045.5
60	16651.0
61	16651.0
62	15466.5
63	14282.0
64	12715.5
65	11149.0
66	9463.5
67	7778.0
68	7778.0
69	6550.0
70	5322.0
71	4534.0
72	3746.0
73	2871.0
74	1996.0
75	1996.0
76	1485.5
77	975.0
78	811.0
79	647.0
80	494.5
81	342.0
82	342.0
83	244.0
84	146.0
85	114.5
86	83.0
87	59.5
88	36.0
89	36.0
90	32.5
91	29.0
92	16.5
93	4.0
94	2.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	267919.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.9220934096941
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.63334797869501	60.201625406228445
2	6.148886296608724	8.352904588076916
3	1.6309150375122796	3.3232549056355145
4	0.8131297610156821	2.209179023276376
5	0.5258350948957764	1.7857910216803143
6	0.36499984752128734	1.487493224271899
7	0.2805478039539821	1.3338775902232842
8	0.2117612596052557	1.1506614444370127
9	0.17892688899270837	1.0937779980901836
>10	1.1319477727550682	14.486803238796503
>50	0.06266246525958116	2.8437907930354047
>100	0.017039793184622953	1.730840766248147
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	301	0.11234738857639809	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	296	0.11048115288576024	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	3.732471381275684E-4	0.0
17	0.0	0.0	0.0	3.732471381275684E-4	0.0
18	0.0	0.0	0.0	7.464942762551368E-4	0.0
19	0.0	0.0	0.0	7.464942762551368E-4	0.0
20	0.0	0.0	0.0	7.464942762551368E-4	0.0
21	0.0	0.0	0.0	0.0011197414143827053	0.0
22	0.0	0.0	0.0	0.0022394828287654106	0.0
23	0.0	0.0	0.0	0.003732471381275684	0.0
24	0.0	0.0	0.0	0.007464942762551368	0.0
25	0.0	0.0	0.0	0.00933117845318921	0.0
26	0.0	0.0	0.0	0.011197414143827052	0.0
27	0.0	0.0	0.0	0.015676379801357873	0.0
28	0.0	0.0	0.0	0.02799353535956763	0.0
29	0.0	0.0	0.0	0.05337434075224228	0.0
30	0.0	0.0	0.0	0.09704425591316779	0.0
31	0.0	0.0	0.0	0.21051138590394858	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAACGC	20	0.001839418	37.0	22
CGCATCG	25	0.005488916	29.599998	13
CCGGTCC	35	8.851577E-4	26.42857	9
CCTATAC	55	1.8956061E-5	23.545454	3
TGGCGCA	40	0.0019273447	23.125	14
GAAGTGT	40	0.0019273447	23.125	6
GTAGGAC	50	2.694614E-4	22.199999	3
CCTACAC	45	0.0038182677	20.555555	3
CTGACCA	45	0.0038182677	20.555555	9
GGTATCA	215	0.0	19.790697	1
TTAGAGC	60	9.211633E-4	18.5	3
GGATAAT	60	9.211633E-4	18.5	1
GTCTTGC	60	9.211633E-4	18.5	1
TATACAC	80	1.6091424E-5	18.5	37
GGCTACA	60	9.211633E-4	18.5	1
CATACAC	50	0.0070211515	18.499998	3
TGAATTA	50	0.0070211515	18.499998	21
ACGGAAT	75	2.05982E-4	17.266666	18
CGGACAT	90	4.425514E-5	16.444445	21
ACCCACG	80	3.3704692E-4	16.1875	14
>>END_MODULE