FastQCFastQC Report
Fri 10 Feb 2017
ERR1630389.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630389.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462362
Sequences flagged as poor quality0
Sequence length43
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT300676.502913301698669No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT205654.447813617901125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT181303.9211699923436614No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87701.8967821750057314No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA57361.240586380368629No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA40890.8843719855870508No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA33500.7245405115472292No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28480.6159675751900027No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA25570.5530298770227657No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22570.4881456521080884No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19330.41807068920023704No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19200.41525903945393433No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA18560.40141707147213657No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA17060.36897495901479793No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA16550.3579446407793028No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA15040.32528624757224855No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13200.2854905896245799No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA12870.27835332488396536No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA11620.25131823116951657No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10890.23552973644027841No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.23358320969283805No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10180.22017380321047147No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA9630.20827836197611393No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9540.20633183522867365No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8940.19335499024573818No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA8930.1931387094960226No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8530.18448747950739897No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA8290.17929674151422478No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA8170.17670137251763768No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7770.16805014252901407No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA7730.1671850195301517No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA7570.16372452753470224No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA7490.16199428153697754No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT7190.15550585904550981No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA7160.15485701679636302No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT7140.15442445529693186No Hit
GAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA6770.146422067557455No Hit
AAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6750.1459895060580238No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA6730.1455569445585926No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6560.14188017181342757No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6320.1366894338202534No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5960.12890332683049213No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA5820.12587539633447384No Hit
GAACTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5670.12263118508873999No Hit
CATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5410.11700788559613462No Hit
GTACATGGGAAGATGGTATCAACGCAAAAAAAAAAAAAAAAAA5280.11419623584983193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTG5240.11333111285096958No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5210.11268227060182283No Hit
GTACATGGGAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5200.11246598985210723No Hit
GTACATGGGAAGCAGTTGTATCAACGCAAAAAAAAAAAAAAAA5070.10965434010580455No Hit
GGTATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAA5040.10900549785665777No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT4960.10727525185893305No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4940.10684269035950186No Hit
TATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAA4910.1061938481103551No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4820.10424732136291477No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4790.10359847911376802No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4660.10078682936746533No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGAGC200.001840798637.024
GCGTTAT451.0775875E-732.888891
TTAGGTC401.5973292E-632.37520
GTCGTCA401.5973292E-632.37524
TATAATC303.5961793E-430.8333343
TCGACCC303.5961793E-430.8333348
CGTTGAA551.8506398E-830.272732
AACAAAC250.005492988529.59999831
CTTCCGT250.005492988529.5999986
GCAACAA250.005492988529.5999982
CACGATC250.005492988529.59999837
GACCCGG250.005492988529.59999810
TGATTAG250.005492988529.59999826
ATTAGGT454.0014475E-628.77777719
ACTGTGC454.0014475E-628.7777778
TATACCA454.0014475E-628.7777775
CGTCATT454.0014475E-628.77777726
GGCAATA405.93257E-527.7529
TATCTCG405.93257E-527.7523
ATTCAAG405.93257E-527.751
Produced by FastQC (version 0.11.5)