##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630389.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	462362
Sequences flagged as poor quality	0
Sequence length	43
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.719914266310813	31.0	31.0	34.0	30.0	34.0
2	31.731044073691177	31.0	31.0	34.0	30.0	34.0
3	31.73038874301954	33.0	31.0	34.0	28.0	34.0
4	35.51406257434651	37.0	35.0	37.0	33.0	37.0
5	35.49939657670829	37.0	35.0	37.0	33.0	37.0
6	35.65890579243104	37.0	35.0	37.0	33.0	37.0
7	35.59042914426359	37.0	35.0	37.0	33.0	37.0
8	35.68934081953102	37.0	35.0	37.0	33.0	37.0
9	37.2375671011026	39.0	37.0	39.0	34.0	39.0
10	37.113104018063765	39.0	37.0	39.0	33.0	39.0
11	37.2634234647311	39.0	37.0	39.0	34.0	39.0
12	37.19568865953517	39.0	37.0	39.0	34.0	39.0
13	37.301871693608035	39.0	37.0	39.0	34.0	39.0
14	38.09581237212401	40.0	37.0	41.0	33.0	41.0
15	38.03241399595988	40.0	37.0	41.0	33.0	41.0
16	37.94828294712801	40.0	37.0	41.0	33.0	41.0
17	38.25263754374278	40.0	38.0	41.0	34.0	41.0
18	38.42900151829086	40.0	38.0	41.0	34.0	41.0
19	38.538573671711774	40.0	38.0	41.0	34.0	41.0
20	38.636910040184965	40.0	38.0	41.0	34.0	41.0
21	38.646504254242345	40.0	38.0	41.0	35.0	41.0
22	38.60869837919206	40.0	38.0	41.0	34.0	41.0
23	38.5223591039056	40.0	38.0	41.0	34.0	41.0
24	38.471641268097294	40.0	38.0	41.0	34.0	41.0
25	38.43018673679931	40.0	38.0	41.0	34.0	41.0
26	38.10918717368642	40.0	37.0	41.0	34.0	41.0
27	37.89417166635666	40.0	36.0	41.0	33.0	41.0
28	37.67100021195513	39.0	36.0	41.0	33.0	41.0
29	37.38142407896843	39.0	35.0	41.0	33.0	41.0
30	36.97059230646117	38.0	35.0	40.0	33.0	41.0
31	36.44982070325849	38.0	35.0	40.0	32.0	41.0
32	36.01716620310493	37.0	35.0	40.0	31.0	41.0
33	35.68809071679766	35.0	35.0	40.0	31.0	41.0
34	35.433279551520236	35.0	35.0	40.0	30.0	41.0
35	35.159693054360005	35.0	35.0	40.0	30.0	41.0
36	34.74626807566366	35.0	34.0	40.0	27.0	41.0
37	34.36278500395794	35.0	34.0	40.0	25.0	41.0
38	33.93414034890411	35.0	33.0	40.0	23.0	41.0
39	33.454042503492936	35.0	33.0	39.0	21.0	41.0
40	32.916725422937006	35.0	33.0	39.0	18.0	41.0
41	32.26870720344665	35.0	33.0	39.0	13.0	41.0
42	31.648340477807434	35.0	32.0	39.0	10.0	41.0
43	30.608527517399786	35.0	30.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	6.0
11	12.0
12	11.0
13	7.0
14	7.0
15	3.0
16	7.0
17	9.0
18	19.0
19	59.0
20	110.0
21	185.0
22	443.0
23	775.0
24	1322.0
25	2071.0
26	3269.0
27	5035.0
28	6830.0
29	9325.0
30	11822.0
31	14654.0
32	18174.0
33	23440.0
34	29934.0
35	38207.0
36	54438.0
37	97472.0
38	81576.0
39	63136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.08870538668835	17.533880379442948	15.648777364921857	13.728636868946841
2	16.525146962769433	32.84720630155593	31.378660010987065	19.248986724687583
3	22.217440014534066	33.25121874202465	31.816412248411417	12.714928995029867
4	13.204156050886533	17.373183782404265	29.68647942521228	39.73618074149692
5	11.232540736479208	47.480761827312804	26.411556313018803	14.87514112318919
6	27.193411223240666	33.16167851164239	19.642185127670526	20.00272513744642
7	22.706883351140448	37.75548163560154	20.604201902405475	18.933433110852533
8	36.84558852154805	28.26227068833511	19.024054744983367	15.868086045133467
9	32.16073120195864	10.118911156193631	21.69620340771949	36.02415423412823
10	21.361400807159757	35.55114823450024	22.824107517486297	20.263343440853703
11	28.22225874963773	31.216016887200936	15.92734697055554	24.634377392605796
12	20.3243346122735	37.87400348644569	26.360946617585356	15.440715283695457
13	33.57412590135003	21.432773454565904	26.241775924492067	18.75132471959201
14	24.24550460461716	25.61499431181628	32.58442519065148	17.555075892915077
15	27.27927468087775	28.554898542700307	28.64508761533171	15.520739161090228
16	16.176286113478184	28.162565262716228	31.985543794689008	23.67560482911658
17	16.917263962003798	31.386013556477394	32.935448847439886	18.761273634078925
18	13.832235348060609	24.491199536294072	38.89960680159702	22.7769583140483
19	14.544231576124336	29.560387748128093	42.54436999580415	13.351010679943421
20	15.749347913539607	27.324693638318028	43.18283076896458	13.743127679177789
21	17.904153022956038	26.9816723692691	40.94389244790878	14.17028215986608
22	17.99434209558744	28.8739126485308	38.54533893356288	14.586406322318876
23	15.33906333132913	30.59010039752402	39.23938385940021	14.83145241174664
24	14.101072319957089	32.354735034453526	39.219918591925804	14.32427405366358
25	14.031646199298386	31.900329179301067	39.205860343194296	14.862164278206254
26	15.104398717887715	31.847340395620748	39.271177129608404	13.777083756883135
27	13.325705832226697	33.36930803136936	39.21278132718519	14.09220480921875
28	12.593162932939991	35.151245128276116	40.00696424014084	12.248627698643054
29	13.47169533828472	34.75263970655028	39.31854261379611	12.457122341368883
30	12.270688335114045	37.05905762151734	39.74612965598384	10.924124387384776
31	12.87778839956571	36.094661758535516	38.7482102767961	12.279339565102669
32	10.68210622845303	37.31448518693145	39.141192399029336	12.862216185586187
33	11.195989289777273	37.118534827689125	40.04438080984164	11.641095072691959
34	10.572019326847796	37.74272107136832	38.34484667857653	13.340412923207356
35	11.062544067202754	38.59724631349462	38.09050051691099	12.249709102391632
36	10.770565055086708	38.83991331467551	37.34433193039221	13.045189699845574
37	11.028155427997975	39.471885665344466	36.842344310302316	12.657614596355238
38	11.180417075797752	38.48131983164706	36.90420060472098	13.434062487834208
39	11.351495148822783	37.78489581756286	37.772135253329644	13.091473780284712
40	10.963920045332445	38.79730600698154	37.445335040509384	12.793438907176627
41	10.765374317093533	38.39826802375627	37.57294068284158	13.263416976308607
42	10.714115779410937	38.530848123331936	37.27815002097923	13.476886076277895
43	10.564882062107179	38.78021982775401	36.73442021619424	13.920477893944572
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	44.0
1	171.0
2	298.0
3	1224.5
4	2151.0
5	2151.0
6	3261.5
7	4372.0
8	4158.5
9	3945.0
10	5413.5
11	6882.0
12	6882.0
13	14466.0
14	22050.0
15	39118.5
16	56187.0
17	45891.0
18	35595.0
19	35595.0
20	38116.0
21	40637.0
22	27164.0
23	13691.0
24	14400.5
25	15110.0
26	15110.0
27	14785.0
28	14460.0
29	14202.0
30	13944.0
31	13261.5
32	12579.0
33	12579.0
34	12072.0
35	11565.0
36	10782.0
37	9999.0
38	10924.0
39	11849.0
40	11849.0
41	13816.5
42	15784.0
43	17373.0
44	18962.0
45	22833.5
46	26705.0
47	26705.0
48	30605.0
49	34505.0
50	28684.5
51	22864.0
52	19896.0
53	16928.0
54	16928.0
55	16100.5
56	15273.0
57	13749.5
58	12226.0
59	10365.0
60	8504.0
61	8504.0
62	7054.0
63	5604.0
64	4390.0
65	3176.0
66	2814.5
67	2453.0
68	2453.0
69	2243.0
70	2033.0
71	1507.0
72	981.0
73	732.5
74	484.0
75	484.0
76	379.5
77	275.0
78	214.0
79	153.0
80	110.5
81	68.0
82	68.0
83	41.0
84	14.0
85	10.5
86	7.0
87	5.5
88	4.0
89	4.0
90	2.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	462362.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.74408790232332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.69227207901838	15.588830660034555
2	11.484404911917881	4.994358198212321
3	4.756621439479377	3.102851840943458
4	2.599722000135831	2.261143347702292
5	1.612826190855986	1.7534717232570927
6	1.1028811180755997	1.4388686386349077
7	0.86290698473765	1.3134187729364961
8	0.6599288643261779	1.147964098815105
9	0.5159443848364619	1.0096866050934743
>10	3.868582994054701	17.060311913969198
>50	0.4719491272147374	7.099331764882962
>100	0.3209654021947685	13.65881601890308
>500	0.028996874341583448	4.372482195313179
>1k	0.016998167717479952	7.094548581137902
>5k	0.001999784437350582	3.1538574275378135
>10k+	0.0029996766560258735	14.950058212626166
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30067	6.502913301698669	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20565	4.447813617901125	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18130	3.9211699923436614	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8770	1.8967821750057314	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	5736	1.240586380368629	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	4089	0.8843719855870508	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3350	0.7245405115472292	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2848	0.6159675751900027	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	2557	0.5530298770227657	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2257	0.4881456521080884	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1933	0.41807068920023704	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1920	0.41525903945393433	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1856	0.40141707147213657	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	1706	0.36897495901479793	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	1655	0.3579446407793028	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	1504	0.32528624757224855	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1320	0.2854905896245799	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	1287	0.27835332488396536	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	1162	0.25131823116951657	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1089	0.23552973644027841	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1080	0.23358320969283805	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1018	0.22017380321047147	No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA	963	0.20827836197611393	No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	954	0.20633183522867365	No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	894	0.19335499024573818	No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	893	0.1931387094960226	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	853	0.18448747950739897	No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	829	0.17929674151422478	No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	817	0.17670137251763768	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	777	0.16805014252901407	No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA	773	0.1671850195301517	No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	757	0.16372452753470224	No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA	749	0.16199428153697754	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	719	0.15550585904550981	No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	716	0.15485701679636302	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	714	0.15442445529693186	No Hit
GAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	677	0.146422067557455	No Hit
AAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	675	0.1459895060580238	No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA	673	0.1455569445585926	No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	656	0.14188017181342757	No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	632	0.1366894338202534	No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	596	0.12890332683049213	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA	582	0.12587539633447384	No Hit
GAACTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	567	0.12263118508873999	No Hit
CATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	541	0.11700788559613462	No Hit
GTACATGGGAAGATGGTATCAACGCAAAAAAAAAAAAAAAAAA	528	0.11419623584983193	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTG	524	0.11333111285096958	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	521	0.11268227060182283	No Hit
GTACATGGGAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	520	0.11246598985210723	No Hit
GTACATGGGAAGCAGTTGTATCAACGCAAAAAAAAAAAAAAAA	507	0.10965434010580455	No Hit
GGTATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAA	504	0.10900549785665777	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT	496	0.10727525185893305	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	494	0.10684269035950186	No Hit
TATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAA	491	0.1061938481103551	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	482	0.10424732136291477	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	479	0.10359847911376802	No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	466	0.10078682936746533	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.162807497155908E-4	0.0
11	0.0	0.0	0.0	4.325614994311816E-4	0.0
12	0.0	0.0	0.0	4.325614994311816E-4	0.0
13	0.0	0.0	0.0	6.488422491467724E-4	0.0
14	0.0	0.0	0.0	0.0012976844982935448	0.0
15	0.0	0.0	0.0	0.0025953689965870896	0.0
16	0.0	0.0	0.0	0.009732633737201587	0.0
17	0.0	0.0	0.0	0.017518740726962856	0.0
18	0.0	0.0	0.0	0.020762951972696717	0.0
19	0.0	0.0	0.0	0.02681881296473326	0.0
20	0.0	0.0	0.0	0.03222583170762303	0.0
21	0.0	0.0	0.0	0.0471492034379988	0.0
22	0.0	0.0	0.0	0.07007496290785142	0.0
23	0.0	0.0	0.0	0.1057612866109239	0.0
24	0.0	0.0	0.0	0.15745238579295012	0.0
25	0.0	0.0	0.0	0.17194319602389468	0.0
26	0.0	0.0	0.0	0.19227358649716023	0.0
27	0.0	0.0	0.0	0.21519934596701287	0.0
28	0.0	0.0	0.0	0.24656005467577352	0.0
29	0.0	0.0	0.0	0.3090651913435793	0.0
30	0.0	0.0	0.0	0.44164529091923643	0.0
31	0.0	0.0	0.0	0.7662826962423382	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGAGC	20	0.0018407986	37.0	24
GCGTTAT	45	1.0775875E-7	32.88889	1
TTAGGTC	40	1.5973292E-6	32.375	20
GTCGTCA	40	1.5973292E-6	32.375	24
TATAATC	30	3.5961793E-4	30.833334	3
TCGACCC	30	3.5961793E-4	30.833334	8
CGTTGAA	55	1.8506398E-8	30.27273	2
AACAAAC	25	0.0054929885	29.599998	31
CTTCCGT	25	0.0054929885	29.599998	6
GCAACAA	25	0.0054929885	29.599998	2
CACGATC	25	0.0054929885	29.599998	37
GACCCGG	25	0.0054929885	29.599998	10
TGATTAG	25	0.0054929885	29.599998	26
ATTAGGT	45	4.0014475E-6	28.777777	19
ACTGTGC	45	4.0014475E-6	28.777777	8
TATACCA	45	4.0014475E-6	28.777777	5
CGTCATT	45	4.0014475E-6	28.777777	26
GGCAATA	40	5.93257E-5	27.75	29
TATCTCG	40	5.93257E-5	27.75	23
ATTCAAG	40	5.93257E-5	27.75	1
>>END_MODULE