FastQCFastQC Report
Fri 10 Feb 2017
ERR1630388.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630388.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2074468
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT99180.4780984811527582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT75770.365250271394883No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66810.32205847475111693No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT36490.1759005200369444No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32620.15724513465621065No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT26490.12769538985416984No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC23190.11178769689385423No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA22880.11029333785818822No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA352.3892295E-531.7142856
AAGACGG7000.021.6714295
GACGGAC7000.020.6142867
TCTAACG450.00382683120.5555552
ATACGAC450.00382683120.5555553
CAAGACG7650.020.0718964
CGGACCA7450.019.3691279
ACGGACC7550.019.1125838
GCGCAAG8050.018.6149061
CGCGAAC701.2197982E-418.528
CGTCCTA500.00703674618.49999833
AGACGGA8050.018.3850946
CGCAAGA8000.017.5749992
GCTACGA752.0682767E-417.2666662
CGAAAGC8650.016.46820819
CGGTCGA1251.6594822E-716.2820
CTAGATA5300.016.0566043
TCTAGCG5800.015.94827628
TAGACTG2100.015.8571445
GGACCGT1059.3502695E-615.8571446
Produced by FastQC (version 0.11.5)