##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630388.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2074468
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.891724528891263	33.0	31.0	34.0	30.0	34.0
2	32.075733633876254	33.0	31.0	34.0	30.0	34.0
3	32.21829259357098	34.0	31.0	34.0	30.0	34.0
4	35.86457973803404	37.0	35.0	37.0	35.0	37.0
5	35.78171415514725	37.0	35.0	37.0	35.0	37.0
6	35.84192477300204	37.0	35.0	37.0	35.0	37.0
7	35.811112535840515	37.0	35.0	37.0	35.0	37.0
8	35.79779779683273	37.0	35.0	37.0	35.0	37.0
9	37.503218174491	39.0	37.0	39.0	35.0	39.0
10	37.42453294049366	39.0	37.0	39.0	34.0	39.0
11	37.49545232801856	39.0	37.0	39.0	35.0	39.0
12	37.38450484654379	39.0	37.0	39.0	34.0	39.0
13	37.42049576084085	39.0	37.0	39.0	34.0	39.0
14	38.635287215806656	40.0	38.0	41.0	34.0	41.0
15	38.6239855230353	40.0	38.0	41.0	34.0	41.0
16	38.603815532464225	40.0	38.0	41.0	34.0	41.0
17	38.58338041367715	40.0	38.0	41.0	34.0	41.0
18	38.55081543798217	40.0	38.0	41.0	34.0	41.0
19	38.56302724361137	40.0	38.0	41.0	34.0	41.0
20	38.52203842141696	40.0	38.0	41.0	34.0	41.0
21	38.473444275833614	40.0	38.0	41.0	34.0	41.0
22	38.44185063351182	40.0	38.0	41.0	34.0	41.0
23	38.370488240840544	40.0	38.0	41.0	34.0	41.0
24	38.346823378331216	40.0	38.0	41.0	34.0	41.0
25	38.31521190011126	40.0	38.0	41.0	34.0	41.0
26	38.14864678558551	40.0	38.0	41.0	33.0	41.0
27	38.043174442796904	40.0	38.0	41.0	33.0	41.0
28	37.92985334071193	40.0	37.0	41.0	33.0	41.0
29	37.852499532410235	40.0	37.0	41.0	33.0	41.0
30	37.76776551867756	40.0	37.0	41.0	33.0	41.0
31	37.647341872711465	40.0	37.0	41.0	33.0	41.0
32	37.53399281165099	40.0	37.0	41.0	32.0	41.0
33	37.44365446948326	40.0	36.0	41.0	32.0	41.0
34	37.353609214507046	40.0	36.0	41.0	32.0	41.0
35	37.23723913793802	39.0	36.0	41.0	31.0	41.0
36	37.110452414787794	39.0	36.0	41.0	31.0	41.0
37	37.0446779608073	39.0	36.0	41.0	31.0	41.0
38	36.90583127818795	39.0	35.0	41.0	31.0	41.0
39	36.80713898695955	39.0	35.0	41.0	30.0	41.0
40	36.667891237657074	39.0	35.0	41.0	30.0	41.0
41	36.51402817493449	39.0	35.0	41.0	30.0	41.0
42	36.4081113808456	39.0	35.0	41.0	30.0	41.0
43	35.19642771062268	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	2.0
11	7.0
12	8.0
13	3.0
14	10.0
15	16.0
16	28.0
17	73.0
18	134.0
19	258.0
20	453.0
21	909.0
22	1428.0
23	2547.0
24	3817.0
25	5762.0
26	8642.0
27	12663.0
28	17761.0
29	24516.0
30	32867.0
31	42930.0
32	55280.0
33	72721.0
34	96234.0
35	129600.0
36	181806.0
37	277897.0
38	476533.0
39	629560.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.9976263793898	18.97339462454952	12.407277432093434	25.621701563967243
2	18.219562798751294	21.4873403687114	34.25056448207444	26.042532350462867
3	19.450529003098627	22.917779401755052	30.45079509541723	27.180896499729084
4	14.097493911692057	15.43552371017533	34.60694500951569	35.860037368616915
5	14.503814954002664	36.893410744345054	33.74214497403672	14.860629327615563
6	34.45341166988356	35.32201991064697	15.806944238233609	14.417624181235864
7	29.385461718377915	31.229982819691603	20.751922902642992	18.63263255928749
8	27.93569242813097	33.204850593019515	19.854825429941556	19.00463154890796
9	26.79231494532574	14.109352373717021	19.570752597774465	39.52758008318277
10	17.377949430890233	26.537695447700326	31.6872566846054	24.397098436804036
11	36.640044580104394	21.706529095652478	21.03527265785734	20.61815366638579
12	21.718966019239634	25.81760721303004	28.44869142353606	24.01473534419427
13	29.78739609384189	18.734056153192046	25.488317968751506	25.99022978421456
14	22.93706145382816	20.75655059514054	24.66608306322392	31.640304887807382
15	25.50885335420937	27.67803600730404	21.898867565081744	24.914243073404844
16	25.359754886554047	26.29175287350781	23.586866608691963	24.76162563124618
17	23.970145598775204	26.579248269917876	24.97098051162997	24.47962561967695
18	23.962529188206325	24.4909538252699	26.78363802189284	24.762878964630932
19	24.483048183919927	25.28002360123174	26.406047237171173	23.830880977677168
20	24.80891486395548	24.388710744152238	26.197801074781584	24.6045733171107
21	25.56154156149914	25.141674877607173	25.40492309353531	23.891860467358377
22	25.095349747501526	24.790018452923835	25.693816438720674	24.420815360853965
23	23.74632917933658	24.795272812113755	26.23810056361438	25.22029744493528
24	24.3557866402374	25.512034892801434	26.00107593850568	24.13110252845549
25	24.761191785074534	24.63026665149812	25.917970294070575	24.69057126935677
26	24.42023689929177	25.95041234668358	25.829562085315366	23.79978866870928
27	24.9812482043589	24.815374351399974	25.54380207359188	24.659575370649243
28	23.668718919742314	25.365780527826892	26.7269487887979	24.23855176363289
29	23.52617634979185	25.667496437640878	26.494937497228204	24.311389715339065
30	23.282740442368837	26.455843136649975	26.445382623400317	23.816033797580875
31	24.471093311634597	25.718786696155348	25.54708002244431	24.263039969765742
32	23.52579070875039	25.41210565793254	25.564482074440292	25.49762155887678
33	23.319135315656833	25.16640410939094	26.505831856649515	25.008628718302717
34	24.532747673138367	25.1122215430655	26.00980106706876	24.345229716727374
35	24.84555076289439	25.09554256802226	26.508290318288836	23.550616350794517
36	23.366424548366137	25.989265681610902	26.189316971869413	24.45499279815355
37	24.41850151460519	24.93873127953769	26.240462614993337	24.40230459086378
38	23.550278914883236	24.958447177782446	27.16739906327791	24.323874844056405
39	24.10362560425131	24.50555997971528	26.678165197052934	24.71264921898048
40	23.725504563097623	24.823617428661226	27.505124205338426	23.94575380290272
41	22.723223496337376	25.11125744046184	28.043479099219653	24.122039963981127
42	24.131391759236585	24.721085116762467	27.541181642715145	23.606341481285803
43	23.085436844530744	24.159880991174603	27.750584728229118	25.004097436065535
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	269.0
1	265.5
2	262.0
3	727.0
4	1192.0
5	1192.0
6	1697.0
7	2202.0
8	2022.0
9	1842.0
10	2612.5
11	3383.0
12	3383.0
13	6124.5
14	8866.0
15	14879.0
16	20892.0
17	18902.5
18	16913.0
19	16913.0
20	19207.0
21	21501.0
22	19799.0
23	18097.0
24	20548.5
25	23000.0
26	23000.0
27	26502.0
28	30004.0
29	36656.0
30	43308.0
31	47734.5
32	52161.0
33	52161.0
34	59173.0
35	66185.0
36	74648.5
37	83112.0
38	95162.0
39	107212.0
40	107212.0
41	114598.5
42	121985.0
43	120001.5
44	118018.0
45	127205.0
46	136392.0
47	136392.0
48	144268.5
49	152145.0
50	153045.0
51	153945.0
52	159792.5
53	165640.0
54	165640.0
55	155763.0
56	145886.0
57	138705.5
58	131525.0
59	123709.5
60	115894.0
61	115894.0
62	105724.5
63	95555.0
64	83890.0
65	72225.0
66	62540.5
67	52856.0
68	52856.0
69	46066.5
70	39277.0
71	34220.5
72	29164.0
73	23399.0
74	17634.0
75	17634.0
76	13335.0
77	9036.0
78	7464.0
79	5892.0
80	4877.5
81	3863.0
82	3863.0
83	2973.5
84	2084.0
85	1950.5
86	1817.0
87	1732.0
88	1647.0
89	1647.0
90	1471.0
91	1295.0
92	744.5
93	194.0
94	133.5
95	73.0
96	73.0
97	45.0
98	17.0
99	12.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2074468.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.565797656859175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.52173494781808	33.7729621874531
2	12.739937728438855	11.100510984763066
3	4.132792093634841	5.401451523274885
4	1.7820576813942925	3.105470574419014
5	0.9012495591054723	1.9631827965161275
6	0.5624740017407661	1.4702777128249243
7	0.3730595784312244	1.1376846675521535
8	0.25868814840119025	0.9015964423579051
9	0.20558166500676567	0.8060696297680464
>10	1.1832103114071708	9.929837633798362
>50	0.1534177722947786	4.680577485696591
>100	0.15927101908115796	14.449553199656506
>500	0.019977358929590357	5.957357601860745
>1k	0.006215178333650332	4.1545182350695224
>5k	3.3295598215983926E-4	1.1689493249891272
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9918	0.4780984811527582	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7577	0.365250271394883	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6681	0.32205847475111693	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3649	0.1759005200369444	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3262	0.15724513465621065	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	2649	0.12769538985416984	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	2319	0.11178769689385423	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2288	0.11029333785818822	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	4.820513018277457E-5	0.0	0.0	0.0
4	0.0	9.641026036554914E-5	0.0	0.0	0.0
5	0.0	9.641026036554914E-5	0.0	0.0	0.0
6	0.0	9.641026036554914E-5	0.0	0.0	0.0
7	0.0	9.641026036554914E-5	0.0	0.0	0.0
8	0.0	9.641026036554914E-5	0.0	0.0	4.820513018277457E-5
9	0.0	9.641026036554914E-5	0.0	0.0	4.820513018277457E-5
10	0.0	9.641026036554914E-5	0.0	9.641026036554914E-5	4.820513018277457E-5
11	0.0	9.641026036554914E-5	0.0	1.4461539054832373E-4	4.820513018277457E-5
12	0.0	9.641026036554914E-5	0.0	2.4102565091387287E-4	4.820513018277457E-5
13	0.0	9.641026036554914E-5	0.0	2.4102565091387287E-4	4.820513018277457E-5
14	0.0	9.641026036554914E-5	0.0	2.8923078109664745E-4	4.820513018277457E-5
15	0.0	9.641026036554914E-5	0.0	2.8923078109664745E-4	4.820513018277457E-5
16	0.0	1.4461539054832373E-4	0.0	9.641026036554915E-4	4.820513018277457E-5
17	0.0	1.4461539054832373E-4	0.0	0.001253333384752139	4.820513018277457E-5
18	0.0	1.4461539054832373E-4	0.0	0.0017353846865798845	4.820513018277457E-5
19	0.0	1.4461539054832373E-4	0.0	0.0022174359884076303	4.820513018277457E-5
20	0.0	1.4461539054832373E-4	0.0	0.0024102565091387285	4.820513018277457E-5
21	0.0	1.4461539054832373E-4	0.0	0.003133333461880347	4.820513018277457E-5
22	0.0	1.4461539054832373E-4	0.0	0.004338461716449711	4.820513018277457E-5
23	0.0	1.4461539054832373E-4	0.0	0.005640000231384625	4.820513018277457E-5
24	0.0	1.9282052073109828E-4	0.0	0.008676923432899422	4.820513018277457E-5
25	0.0	1.9282052073109828E-4	0.0	0.009978461947834336	4.820513018277457E-5
26	0.0	1.9282052073109828E-4	0.0	0.01234051332679029	4.820513018277457E-5
27	0.0	1.9282052073109828E-4	0.0	0.022608206055721274	4.820513018277457E-5
28	0.0	1.9282052073109828E-4	0.0	0.05167589955593434	4.820513018277457E-5
29	0.0	2.8923078109664745E-4	0.0	0.09231282430001331	4.820513018277457E-5
30	0.0	2.8923078109664745E-4	0.0	0.15522051918853413	4.820513018277457E-5
31	0.0	2.8923078109664745E-4	0.0	0.33468821885900385	4.820513018277457E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGA	35	2.3892295E-5	31.714285	6
AAGACGG	700	0.0	21.671429	5
GACGGAC	700	0.0	20.614286	7
TCTAACG	45	0.003826831	20.555555	2
ATACGAC	45	0.003826831	20.555555	3
CAAGACG	765	0.0	20.071896	4
CGGACCA	745	0.0	19.369127	9
ACGGACC	755	0.0	19.112583	8
GCGCAAG	805	0.0	18.614906	1
CGCGAAC	70	1.2197982E-4	18.5	28
CGTCCTA	50	0.007036746	18.499998	33
AGACGGA	805	0.0	18.385094	6
CGCAAGA	800	0.0	17.574999	2
GCTACGA	75	2.0682767E-4	17.266666	2
CGAAAGC	865	0.0	16.468208	19
CGGTCGA	125	1.6594822E-7	16.28	20
CTAGATA	530	0.0	16.056604	3
TCTAGCG	580	0.0	15.948276	28
TAGACTG	210	0.0	15.857144	5
GGACCGT	105	9.3502695E-6	15.857144	6
>>END_MODULE