FastQCFastQC Report
Fri 10 Feb 2017
ERR1630387.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630387.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390871
Sequences flagged as poor quality0
Sequence length43
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT159204.072955015849219No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT131283.3586528547781747No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT101412.5944621115406363No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46801.197325971995876No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA26320.6733679398062276No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA16900.4323677121096219No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15080.38580503542089334No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA14060.359709469364573No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13210.33796316431763934No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA10630.2719567325281231No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9500.24304693875984662No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9020.23076667238040172No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA8380.21439298387447522No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA7190.1839481568087681No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA7030.1798547346822865No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.17857554026776098No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA6960.17806386250195078No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA6530.16706279053703138No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6390.16348104617635997No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5690.14557232437300285No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5560.14224641889523654No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5500.14071138559780594No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5490.14045554671490082No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5450.13943219118328043No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5440.1391763523003753No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5230.13380373575936816No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5220.13354789687646307No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT5210.13329205799355798No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5190.13278038022774777No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5140.13150118581322226No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5040.12894279698417124No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA4830.12357018044316412No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4790.1225468249115437No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4730.1210117916141131No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4670.1194767583166825No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4640.11870924166796717No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA4640.11870924166796717No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4520.11563917507310596No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4450.11384830289277025No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA4310.11026655853209884No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4100.10489394199109169No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4040.10335890869366109No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA200.001840450537.010
TACACTG303.595172E-430.8333325
AGTCCTA250.005491962729.59999829
ATTACAT250.005491962729.5999985
ATATGCT250.005491962729.59999835
CAATGCG250.005491962729.59999819
GGCCCAC405.930256E-527.7525
ATACACT358.8588835E-426.428574
ACGTTTA451.3210304E-424.66666626
TTGATCA400.001928919923.12520
GCCCACC400.001928919923.12526
GAGTCCT400.001928919923.12528
AAAACCC400.001928919923.12537
CCGCTTA855.158654E-821.76470625
ACAGTTT603.7181933E-521.58333232
AGTTTTA806.935952E-720.812534
ACTGCGG450.003821359520.5555557
AGTTTTT450.003821359520.55555515
CCAATGC450.003821359520.55555518
ATCACGT450.003821359520.55555523
Produced by FastQC (version 0.11.5)