##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630387.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	390871
Sequences flagged as poor quality	0
Sequence length	43
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.719966433938563	31.0	31.0	34.0	30.0	34.0
2	31.794758372967042	31.0	31.0	34.0	30.0	34.0
3	31.82007107204167	33.0	31.0	34.0	30.0	34.0
4	35.57173850196101	37.0	35.0	37.0	33.0	37.0
5	35.565864441209506	37.0	35.0	37.0	33.0	37.0
6	35.694556516088426	37.0	35.0	37.0	33.0	37.0
7	35.66717152206227	37.0	35.0	37.0	33.0	37.0
8	35.73006695303565	37.0	35.0	37.0	33.0	37.0
9	37.30279810986233	39.0	37.0	39.0	34.0	39.0
10	37.21715348542102	39.0	37.0	39.0	34.0	39.0
11	37.34276526014977	39.0	37.0	39.0	34.0	39.0
12	37.23984383594588	39.0	37.0	39.0	34.0	39.0
13	37.31844009916315	39.0	37.0	39.0	34.0	39.0
14	38.23330203570999	40.0	38.0	41.0	34.0	41.0
15	38.21764981285386	40.0	38.0	41.0	33.0	41.0
16	38.094859941003556	40.0	37.0	41.0	33.0	41.0
17	38.313960360323485	40.0	38.0	41.0	34.0	41.0
18	38.430950364698326	40.0	38.0	41.0	34.0	41.0
19	38.48396785640275	40.0	38.0	41.0	34.0	41.0
20	38.54162114866541	40.0	38.0	41.0	34.0	41.0
21	38.530771021641414	40.0	38.0	41.0	34.0	41.0
22	38.48466117977542	40.0	38.0	41.0	34.0	41.0
23	38.42096497309854	40.0	38.0	41.0	34.0	41.0
24	38.366499433316875	40.0	38.0	41.0	34.0	41.0
25	38.328085225048675	40.0	38.0	41.0	34.0	41.0
26	38.049369229234195	40.0	37.0	41.0	33.0	41.0
27	37.85302056177102	40.0	37.0	41.0	33.0	41.0
28	37.695316357570654	40.0	36.0	41.0	33.0	41.0
29	37.48705583171942	39.0	36.0	41.0	33.0	41.0
30	37.193772369912324	39.0	35.0	40.0	33.0	41.0
31	36.82727549498428	38.0	35.0	40.0	32.0	41.0
32	36.505509490343364	38.0	35.0	40.0	31.0	41.0
33	36.273701553709536	38.0	35.0	40.0	31.0	41.0
34	36.045703058042164	38.0	35.0	40.0	31.0	41.0
35	35.819070742009515	38.0	35.0	40.0	30.0	41.0
36	35.51422080430628	37.0	35.0	40.0	30.0	41.0
37	35.2393705340125	37.0	35.0	40.0	28.0	41.0
38	34.919436335773185	37.0	34.0	40.0	26.0	41.0
39	34.56030506228397	36.0	34.0	40.0	24.0	41.0
40	34.144589903062645	36.0	33.0	40.0	22.0	41.0
41	33.68371406423091	35.0	33.0	40.0	20.0	41.0
42	33.240872308255156	35.0	33.0	40.0	15.0	41.0
43	32.19138283474599	35.0	32.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	9.0
11	14.0
12	4.0
13	1.0
14	5.0
15	5.0
16	2.0
17	13.0
18	14.0
19	33.0
20	85.0
21	164.0
22	336.0
23	607.0
24	971.0
25	1600.0
26	2346.0
27	3644.0
28	5075.0
29	6683.0
30	8438.0
31	10884.0
32	13625.0
33	17683.0
34	23511.0
35	29960.0
36	43829.0
37	73442.0
38	80777.0
39	67104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.91844112251868	18.888840563766564	14.770857904526046	17.421860409188707
2	18.0632484886318	27.923534874677326	32.44267290231304	21.57054373437784
3	22.156670615113427	28.873976324669776	31.612987405051797	17.356365655165003
4	13.756712572690223	18.13309250366489	31.684366453382317	36.425828470262566
5	12.144671771505177	43.23472449989894	29.779134292388026	14.841469436207856
6	28.224913078739533	33.85976447472439	18.79290098267715	19.12242146385892
7	24.03631888781721	35.913383187803646	20.691993010481717	19.358304913897424
8	32.976352812053086	30.143960539410703	19.53611293751647	17.343573711019747
9	29.564997147396454	11.63580823340693	21.47946509206362	37.31972952713299
10	21.627084127499867	31.330029600558753	25.6406845225151	21.40220174942628
11	30.619053344965476	27.556406077708502	17.65160372603749	24.172936851288533
12	20.463273049164556	33.411278912991754	27.4561172356097	18.669330802233986
13	33.23219169495818	20.45483036602869	26.293329512806014	20.019648426207112
14	23.10020441526744	24.77569325941295	30.132703628562872	21.991398696756733
15	28.399139357997903	27.38678489834242	26.272094885524893	17.94198085813478
16	18.960731289862895	27.264238073430874	30.29592883585633	23.479101800849897
17	18.71717267333724	30.225828981940335	30.00811009258809	21.048888252134333
18	17.115109588585494	24.530855448472767	34.69303171634631	23.661003246595424
19	17.540057973090867	28.784176876770083	36.89017604273533	16.785589107403723
20	19.013945777507157	25.644777944641582	38.19162844007358	17.14964783777768
21	20.710925087816697	26.11040471152887	35.91440654333527	17.26426365731917
22	20.71655354324061	27.124805882247593	34.15193247900202	18.006708095509772
23	19.05257744882583	28.33492379838873	34.737803520854705	17.87469523193074
24	17.790780078337868	29.405865362229484	35.2039419655078	17.59941259392485
25	17.786174978445572	29.503339976616328	35.08625607937145	17.624228965566644
26	18.71819602886886	29.46087072205408	34.812764313545905	17.00816893553116
27	17.346387938731706	30.200756771415634	35.09725715133637	17.35559813851629
28	16.24193148123038	31.92178493671825	35.142028955844765	16.6942546262066
29	17.176766759365623	31.44566877563186	34.63137454556618	16.746189919436336
30	16.050052319051556	33.38902093017901	35.77855609651266	14.782370654256773
31	17.007145579999538	31.87112883790304	34.543110130963925	16.578615451133494
32	14.915406873367429	32.84766585395181	34.917402416654085	17.319524856026668
33	15.802911958165225	32.41580981960801	35.72662080328293	16.054657418943847
34	14.948665928145092	32.93925617403184	34.463288399497536	17.648789498325534
35	15.761210220251694	33.6942367174848	34.13914053485677	16.40541252740674
36	15.192224544670749	33.842623269569756	33.92577090651391	17.039381279245582
37	15.554748241747276	34.44691471099161	33.41051139634304	16.587825650918077
38	15.505115498463686	33.27901021052982	33.51617285498285	17.69970143602365
39	15.761210220251694	32.42527585827549	34.65081830066698	17.16269562080584
40	15.428875511357967	33.33887650912961	34.07646000854502	17.155787970967403
41	15.033860276152492	32.61459663162527	34.49757080980682	17.853972282415427
42	14.657009601633275	33.020357099912765	34.41902827275495	17.903605025699015
43	14.733249588738994	33.437374479048074	33.68477067881731	18.14460525339562
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	54.0
1	109.0
2	164.0
3	645.5
4	1127.0
5	1127.0
6	1689.0
7	2251.0
8	2264.5
9	2278.0
10	3184.5
11	4091.0
12	4091.0
13	8220.5
14	12350.0
15	21450.5
16	30551.0
17	25593.5
18	20636.0
19	20636.0
20	22958.5
21	25281.0
22	17246.5
23	9212.0
24	9416.0
25	9620.0
26	9620.0
27	9527.5
28	9435.0
29	9323.0
30	9211.0
31	9144.0
32	9077.0
33	9077.0
34	9493.5
35	9910.0
36	9596.0
37	9282.0
38	10348.5
39	11415.0
40	11415.0
41	13305.5
42	15196.0
43	17533.5
44	19871.0
45	23850.0
46	27829.0
47	27829.0
48	32105.5
49	36382.0
50	31479.5
51	26577.0
52	24286.5
53	21996.0
54	21996.0
55	20591.5
56	19187.0
57	17627.0
58	16067.0
59	13518.0
60	10969.0
61	10969.0
62	9159.5
63	7350.0
64	6040.5
65	4731.0
66	4069.0
67	3407.0
68	3407.0
69	2925.0
70	2443.0
71	1872.0
72	1301.0
73	1024.5
74	748.0
75	748.0
76	605.0
77	462.0
78	349.5
79	237.0
80	172.0
81	107.0
82	107.0
83	73.0
84	39.0
85	27.0
86	15.0
87	10.5
88	6.0
89	6.0
90	5.0
91	4.0
92	3.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	390871.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.131473674135325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.12559923318828	18.32485250245024
2	12.469854937136057	6.517113720201145
3	5.152367699829023	4.039168827226419
4	2.7220911943760577	2.845290175377342
5	1.7170439342982913	2.243444418322477
6	1.1516803612137572	1.8057063024045577
7	0.8650083020349197	1.5822759170773804
8	0.6522521803926365	1.3635448544662039
9	0.4774525888461453	1.122888578047272
>10	3.8945348177054946	20.75412353690031
>50	0.4674640406964094	8.465775945994233
>100	0.27368621186071834	13.525299931230473
>500	0.02097595054406965	3.4490584988088977
>1k	0.006991983514689884	3.732521305658184
>5k	0.0	0.0
>10k+	0.0029965643634385216	10.228935485834864
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15920	4.072955015849219	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13128	3.3586528547781747	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10141	2.5944621115406363	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4680	1.197325971995876	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	2632	0.6733679398062276	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1690	0.4323677121096219	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1508	0.38580503542089334	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1406	0.359709469364573	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1321	0.33796316431763934	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	1063	0.2719567325281231	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	950	0.24304693875984662	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	902	0.23076667238040172	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	838	0.21439298387447522	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	719	0.1839481568087681	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	703	0.1798547346822865	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	698	0.17857554026776098	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	696	0.17806386250195078	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	653	0.16706279053703138	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	639	0.16348104617635997	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	569	0.14557232437300285	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	556	0.14224641889523654	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	550	0.14071138559780594	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	549	0.14045554671490082	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	545	0.13943219118328043	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	544	0.1391763523003753	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	523	0.13380373575936816	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	522	0.13354789687646307	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	521	0.13329205799355798	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	519	0.13278038022774777	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	514	0.13150118581322226	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	504	0.12894279698417124	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	483	0.12357018044316412	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	479	0.1225468249115437	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	473	0.1210117916141131	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	467	0.1194767583166825	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	464	0.11870924166796717	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	464	0.11870924166796717	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	452	0.11563917507310596	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	445	0.11384830289277025	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	431	0.11026655853209884	No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	410	0.10489394199109169	No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	404	0.10335890869366109	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.5583888290510166E-4	0.0
3	0.0	0.0	0.0	2.5583888290510166E-4	0.0
4	0.0	0.0	0.0	2.5583888290510166E-4	0.0
5	0.0	0.0	0.0	2.5583888290510166E-4	0.0
6	0.0	0.0	0.0	2.5583888290510166E-4	0.0
7	0.0	0.0	0.0	2.5583888290510166E-4	0.0
8	0.0	0.0	0.0	2.5583888290510166E-4	0.0
9	0.0	2.5583888290510166E-4	0.0	2.5583888290510166E-4	0.0
10	0.0	2.5583888290510166E-4	0.0	2.5583888290510166E-4	0.0
11	0.0	2.5583888290510166E-4	0.0	2.5583888290510166E-4	0.0
12	0.0	2.5583888290510166E-4	0.0	7.675166487153051E-4	0.0
13	0.0	2.5583888290510166E-4	0.0	0.0010233555316204066	0.0
14	0.0	2.5583888290510166E-4	0.0	0.0010233555316204066	0.0
15	0.0	2.5583888290510166E-4	0.0	0.0020467110632408133	0.0
16	0.0	2.5583888290510166E-4	0.0	0.0043492610093867285	0.0
17	0.0	2.5583888290510166E-4	0.0	0.0069076498384377455	0.0
18	0.0	2.5583888290510166E-4	0.0	0.00767516648715305	0.0
19	0.0	2.5583888290510166E-4	0.0	0.009721877550393864	0.0
20	0.0	2.5583888290510166E-4	0.0	0.012280266379444882	0.0
21	0.0	2.5583888290510166E-4	0.0	0.016373688505926506	0.0
22	0.0	2.5583888290510166E-4	0.0	0.030188988182802	0.0
23	0.0	2.5583888290510166E-4	0.0	0.04298093232805708	0.0
24	0.0	2.5583888290510166E-4	0.0	0.059866298599793796	0.0
25	0.0	2.5583888290510166E-4	0.0	0.06728562620404174	0.0
26	0.0	2.5583888290510166E-4	0.0	0.08110092588091723	0.0
27	0.0	5.116777658102033E-4	0.0	0.09798629215265395	0.0
28	0.0	5.116777658102033E-4	0.0	0.12817528033545594	0.0
29	0.0	5.116777658102033E-4	0.0	0.1824131235113375	0.0
30	0.0	5.116777658102033E-4	0.0	0.30009900964768427	0.0
31	0.0	5.116777658102033E-4	0.0	0.5920111750424053	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTCA	20	0.0018404505	37.0	10
TACACTG	30	3.595172E-4	30.833332	5
AGTCCTA	25	0.0054919627	29.599998	29
ATTACAT	25	0.0054919627	29.599998	5
ATATGCT	25	0.0054919627	29.599998	35
CAATGCG	25	0.0054919627	29.599998	19
GGCCCAC	40	5.930256E-5	27.75	25
ATACACT	35	8.8588835E-4	26.42857	4
ACGTTTA	45	1.3210304E-4	24.666666	26
TTGATCA	40	0.0019289199	23.125	20
GCCCACC	40	0.0019289199	23.125	26
GAGTCCT	40	0.0019289199	23.125	28
AAAACCC	40	0.0019289199	23.125	37
CCGCTTA	85	5.158654E-8	21.764706	25
ACAGTTT	60	3.7181933E-5	21.583332	32
AGTTTTA	80	6.935952E-7	20.8125	34
ACTGCGG	45	0.0038213595	20.555555	7
AGTTTTT	45	0.0038213595	20.555555	15
CCAATGC	45	0.0038213595	20.555555	18
ATCACGT	45	0.0038213595	20.555555	23
>>END_MODULE