FastQCFastQC Report
Fri 10 Feb 2017
ERR1630386.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630386.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1720320
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT112600.6545293898809524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT86130.5006626674107143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81570.47415597098214285No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33240.19321986607142858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC953.6084948E-617.5263163
GGTATCA37200.016.9086021
CTTATAC10200.016.50490237
TATCGCG904.448023E-516.4444457
TCTAGCG2200.015.97727328
TACGGTG700.00259319615.85714228
TATACTA5150.015.8058265
TCGCGCG855.366075E-415.2352949
TCTTATA16650.015.11111137
AACGCGC1152.2109958E-514.47826136
GTACCTA908.276961E-414.3888881
GCGAATC908.276961E-414.38888820
TAAACCG1803.3323886E-914.3888885
GCGCGTA1702.4387191E-814.14705920
CGAGCCG2750.014.12727415
ACTATCG2650.013.96226411
CTCTATG3450.013.9420291
GTGTTAT950.001245513813.6315791
ATCGGTC3400.013.602941514
CGCATCG2254.0745363E-1013.15555613
Produced by FastQC (version 0.11.5)