##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630386.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1720320
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.876600864955357	33.0	31.0	34.0	30.0	34.0
2	32.054239908854164	33.0	31.0	34.0	30.0	34.0
3	32.20236583891369	34.0	31.0	34.0	30.0	34.0
4	35.84305652436756	37.0	35.0	37.0	35.0	37.0
5	35.77266031901042	37.0	35.0	37.0	35.0	37.0
6	35.839825148809524	37.0	35.0	37.0	35.0	37.0
7	35.81826462518601	37.0	35.0	37.0	35.0	37.0
8	35.811906505766366	37.0	35.0	37.0	35.0	37.0
9	37.515071614583334	39.0	37.0	39.0	35.0	39.0
10	37.430266462053574	39.0	37.0	39.0	34.0	39.0
11	37.50757359095982	39.0	37.0	39.0	35.0	39.0
12	37.38335890997024	39.0	37.0	39.0	34.0	39.0
13	37.42970203218006	39.0	37.0	39.0	34.0	39.0
14	38.62788609095982	40.0	38.0	41.0	34.0	41.0
15	38.62453845796131	40.0	38.0	41.0	34.0	41.0
16	38.612041945684524	40.0	38.0	41.0	34.0	41.0
17	38.60544026692708	40.0	38.0	41.0	34.0	41.0
18	38.59527355375744	40.0	38.0	41.0	34.0	41.0
19	38.61318591889881	40.0	38.0	41.0	34.0	41.0
20	38.57318347749256	40.0	38.0	41.0	34.0	41.0
21	38.53836669921875	40.0	38.0	41.0	34.0	41.0
22	38.50945579892113	40.0	38.0	41.0	34.0	41.0
23	38.4447509765625	40.0	38.0	41.0	34.0	41.0
24	38.434469749813985	40.0	38.0	41.0	34.0	41.0
25	38.40477876209077	40.0	38.0	41.0	34.0	41.0
26	38.248507254464286	40.0	38.0	41.0	34.0	41.0
27	38.153863816034224	40.0	38.0	41.0	33.0	41.0
28	38.067728678385414	40.0	37.0	41.0	33.0	41.0
29	37.99263392857143	40.0	37.0	41.0	33.0	41.0
30	37.90227573939732	40.0	37.0	41.0	33.0	41.0
31	37.774561128162205	40.0	37.0	41.0	33.0	41.0
32	37.66682477678572	40.0	37.0	41.0	33.0	41.0
33	37.57340436662946	40.0	37.0	41.0	33.0	41.0
34	37.473553176153274	40.0	36.0	41.0	32.0	41.0
35	37.381505766369045	40.0	36.0	41.0	32.0	41.0
36	37.26417003813244	39.0	36.0	41.0	32.0	41.0
37	37.18668271019345	39.0	36.0	41.0	31.0	41.0
38	37.0730480375744	39.0	36.0	41.0	31.0	41.0
39	36.96341262090774	39.0	35.0	41.0	31.0	41.0
40	36.842689150855655	39.0	35.0	41.0	31.0	41.0
41	36.69427955264137	39.0	35.0	41.0	30.0	41.0
42	36.598849051339286	39.0	35.0	41.0	30.0	41.0
43	35.461819893973214	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	8.0
11	6.0
12	2.0
13	3.0
14	4.0
15	4.0
16	8.0
17	29.0
18	46.0
19	107.0
20	230.0
21	474.0
22	859.0
23	1614.0
24	2622.0
25	4201.0
26	6535.0
27	9841.0
28	13910.0
29	19672.0
30	26436.0
31	34538.0
32	45016.0
33	58655.0
34	78223.0
35	105017.0
36	148247.0
37	231147.0
38	395495.0
39	537369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.001708984375	19.43574451264881	12.682117280505953	24.880429222470237
2	17.45088123139881	21.560174851190474	35.60994466145833	25.378999255952383
3	19.108944847470237	24.503348214285715	30.237978980654763	26.149727957589285
4	13.653796968005953	15.997488839285715	35.707891555059526	34.64082263764881
5	13.84666806175595	37.88242885044643	34.06889415922619	14.202008928571427
6	32.6904296875	36.276681082589285	16.69956752232143	14.333321707589286
7	28.664725167410715	31.605573381696427	21.64975120907738	18.079950241815474
8	27.930791945684526	34.017799014136905	19.896240234375	18.15516880580357
9	27.231329055059522	14.107666015625	20.21908714657738	38.441917782738095
10	17.224411737351193	27.199183872767858	32.34915597098214	23.22724841889881
11	36.03910900297619	22.503836495535715	21.562965029761905	19.89408947172619
12	21.441359747023807	26.402529761904763	29.767310732886905	22.388799758184526
13	30.043712797619047	20.055396670386905	25.557745070684522	24.343145461309522
14	22.260219029017858	20.649239676339285	26.337890625	30.752650669642854
15	24.97982933407738	28.290201822916668	22.804071335565474	23.925897507440478
16	24.053780691964285	26.698404947916664	24.910074869791668	24.33773949032738
17	22.900913783482142	27.07536969866071	26.267089843750004	23.756626674107142
18	23.023332868303573	25.557745070684522	27.7294921875	23.689429873511905
19	23.585728236607142	25.942847842261905	27.728969029017858	22.742454892113095
20	23.73477027529762	25.411725725446427	27.727806454613095	23.125697544642858
21	24.621988932291668	25.32534644717262	27.18965076264881	22.863013857886905
22	24.421735491071427	25.42788550967262	26.96161179315476	23.18876720610119
23	23.334030877976193	25.616513206845237	27.57585797991071	23.473597935267858
24	23.176327659970237	26.314057849702383	27.032238188244044	23.477376302083332
25	23.60502697172619	25.685163225446427	27.34276181175595	23.36704799107143
26	23.254162016369047	26.342366536458332	27.535865420386905	22.867606026785715
27	23.530738467261905	25.93168712797619	27.15692429315476	23.380650111607142
28	23.040073939732142	26.053234281994047	27.903878348214285	23.002813430059526
29	23.115292503720237	26.310977027529763	27.440941220238095	23.132789248511905
30	22.6708984375	26.833321707589285	27.56039574032738	22.935384114583332
31	23.131626674107142	26.374104817708332	26.987072172619047	23.507196335565474
32	22.690022786458332	26.19936988467262	27.121117001488095	23.989490327380953
33	22.444603329613095	26.114095052083336	27.920038132440478	23.521263485863095
34	23.34774925595238	25.82920619419643	27.546735491071427	23.276309058779763
35	23.304210844494047	26.140950520833332	27.717808314732146	22.837030319940478
36	22.740827287946427	26.156761532738095	27.50517345610119	23.597237723214285
37	22.93939499627976	25.76084681919643	27.6123046875	23.687453497023807
38	22.489827473958332	25.616396949404763	28.180047898065474	23.713727678571427
39	22.691417875744047	25.27070545014881	28.167782738095237	23.870093936011905
40	22.408970424107146	25.60442243303571	28.636765252976193	23.349841889880953
41	21.834949311755953	25.590006510416668	28.807663690476193	23.767380487351193
42	22.185639880952383	25.604015531994044	28.76924060639881	23.441103980654763
43	21.789085751488095	24.932861328125	28.932407924107146	24.345644996279763
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	235.0
1	288.5
2	342.0
3	921.5
4	1501.0
5	1501.0
6	2094.5
7	2688.0
8	2580.0
9	2472.0
10	3359.5
11	4247.0
12	4247.0
13	7211.5
14	10176.0
15	16810.5
16	23445.0
17	22162.0
18	20879.0
19	20879.0
20	22706.0
21	24533.0
22	21148.5
23	17764.0
24	20258.0
25	22752.0
26	22752.0
27	27023.0
28	31294.0
29	35321.0
30	39348.0
31	43933.5
32	48519.0
33	48519.0
34	55280.5
35	62042.0
36	68570.5
37	75099.0
38	82777.0
39	90455.0
40	90455.0
41	97836.0
42	105217.0
43	109162.0
44	113107.0
45	117742.5
46	122378.0
47	122378.0
48	125667.5
49	128957.0
50	127988.5
51	127020.0
52	124673.5
53	122327.0
54	122327.0
55	118877.5
56	115428.0
57	107944.5
58	100461.0
59	94030.5
60	87600.0
61	87600.0
62	77841.5
63	68083.0
64	59574.5
65	51066.0
66	43647.5
67	36229.0
68	36229.0
69	30549.0
70	24869.0
71	21012.0
72	17155.0
73	13463.0
74	9771.0
75	9771.0
76	7530.5
77	5290.0
78	4173.5
79	3057.0
80	2491.5
81	1926.0
82	1926.0
83	1423.5
84	921.0
85	790.0
86	659.0
87	591.0
88	523.0
89	523.0
90	462.5
91	402.0
92	227.5
93	53.0
94	37.0
95	21.0
96	21.0
97	14.5
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1720320.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.9427072696359
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.49719763458371	44.2273396566296
2	11.052222757165035	12.144780792514535
3	3.320532461806492	5.473171290850726
4	1.5050888422240722	3.307746226924497
5	0.8544211876113773	2.3472106597953277
6	0.5111667858304456	1.6850932247905706
7	0.3514260927361333	1.3515810658079306
8	0.2555835897024576	1.1233963481555884
9	0.21278357992721514	1.052181534935354
>10	1.2581480319367457	13.132618999491925
>50	0.11256494389601072	4.286292066196597
>100	0.06462654694542716	6.493247863754529
>500	0.003495975148798412	1.3558402526804516
>1k	4.2375456349071663E-4	0.38799824522915305
>5k	2.1187728174535832E-4	0.9761071965935939
>10k+	1.0593864087267916E-4	0.6553945756496043
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11260	0.6545293898809524	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8613	0.5006626674107143	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8157	0.47415597098214285	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3324	0.19321986607142858	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.1625744047619047E-4	0.0
11	0.0	0.0	0.0	1.1625744047619047E-4	0.0
12	0.0	0.0	0.0	1.1625744047619047E-4	0.0
13	0.0	0.0	0.0	1.7438616071428572E-4	0.0
14	0.0	0.0	0.0	3.4877232142857144E-4	0.0
15	0.0	0.0	0.0	5.812872023809524E-4	0.0
16	0.0	0.0	0.0	8.719308035714286E-4	0.0
17	0.0	0.0	0.0	0.0015694754464285715	0.0
18	0.0	0.0	0.0	0.0017438616071428572	0.0
19	0.0	0.0	0.0	0.002499534970238095	0.0
20	0.0	0.0	0.0	0.002732049851190476	0.0
21	0.0	0.0	0.0	0.004417782738095238	0.0
22	0.0	0.0	0.0	0.006626674107142857	0.0
23	0.0	0.0	0.0	0.008951822916666666	0.0
24	0.0	0.0	0.0	0.014706566220238096	0.0
25	0.0	0.0	0.0	0.016450427827380952	0.0
26	0.0	0.0	0.0	0.019880022321428572	0.0
27	0.0	0.0	0.0	0.03278459821428571	0.0
28	0.0	0.0	0.0	0.0670805431547619	0.0
29	0.0	0.0	0.0	0.11643182663690477	0.0
30	0.0	0.0	0.0	0.18763950892857142	0.0
31	0.0	0.0	0.0	0.4332914806547619	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGTAC	95	3.6084948E-6	17.526316	3
GGTATCA	3720	0.0	16.908602	1
CTTATAC	1020	0.0	16.504902	37
TATCGCG	90	4.448023E-5	16.444445	7
TCTAGCG	220	0.0	15.977273	28
TACGGTG	70	0.002593196	15.857142	28
TATACTA	515	0.0	15.805826	5
TCGCGCG	85	5.366075E-4	15.235294	9
TCTTATA	1665	0.0	15.111111	37
AACGCGC	115	2.2109958E-5	14.478261	36
GTACCTA	90	8.276961E-4	14.388888	1
GCGAATC	90	8.276961E-4	14.388888	20
TAAACCG	180	3.3323886E-9	14.388888	5
GCGCGTA	170	2.4387191E-8	14.147059	20
CGAGCCG	275	0.0	14.127274	15
ACTATCG	265	0.0	13.962264	11
CTCTATG	345	0.0	13.942029	1
GTGTTAT	95	0.0012455138	13.631579	1
ATCGGTC	340	0.0	13.6029415	14
CGCATCG	225	4.0745363E-10	13.155556	13
>>END_MODULE