FastQCFastQC Report
Fri 10 Feb 2017
ERR1630385.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630385.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985878
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16000.16229188601429384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15640.15864031857897226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11200.11360432021000569No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG358.868496E-426.4285725
TCTATAC1201.0913936E-1121.5833343
TATTAGA1500.020.9666652
CGGTTCG656.900859E-519.92307724
GTACTAG656.900859E-519.9230771
TATACTG759.263582E-619.7333325
TAGACTA1103.849891E-818.55
GTTTAGC902.1515843E-618.51
CGTGTCG500.007034188618.59
TCTAGCG1451.546141E-1017.8620728
CGCAATA1351.1514203E-917.81481636
ACGTTAA650.001579718717.07692320
GCGTTTG650.001579718717.07692327
GGTATCA10850.017.0506941
TCTTATA8550.016.2280737
TAGGACG803.381955E-416.18754
GACGGAT700.002592142715.8571437
AAAGCCG700.002592142715.85714315
CGCCAAA700.002592142715.85714324
GTACTAT1059.340323E-615.8571421
Produced by FastQC (version 0.11.5)