FastQCFastQC Report
Fri 10 Feb 2017
ERR1630384.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630384.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494910
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50300.33647510552474735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47750.3194172224414848No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35750.2391448314614258No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17500.11706390351258604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA451.3234718E-424.66666813
TGTACGT450.003826337220.55555712
TACGTAG450.003826337220.55555714
GACGGAC3000.019.1166677
AAGACGG3300.018.55
CAAGACG3300.018.54
TCGTTTA1351.1532393E-917.81481630
CTAGTAC852.724002E-517.4117643
CGTAGAC650.001580234517.0769233
AGACGGA3800.017.0394746
GGTATCA20350.017.01
CGCAAGA3550.016.6760582
ACGGACC3450.016.6231888
CGGACCA3350.016.5671639
TAGTACT1801.0913936E-1116.4444454
GCGCAAG3650.016.2191771
GCGGGTA1455.3483745E-815.31034623
TACCGTC1455.3483745E-815.3103467
CGAAAGC3650.015.2054819
CGATGCG2201.8189894E-1215.13636433
Produced by FastQC (version 0.11.5)