FastQCFastQC Report
Fri 10 Feb 2017
ERR1630383.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630383.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146759
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54383.705394558425718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35182.397127263063935No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32232.1961174442453277No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18031.228544757050675No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8070.5498810975817497No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA7560.5151302475487023No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6740.4592563318092928No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5600.3815779611471869No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.36386184152249607No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4200.2861834708603902No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4120.28073235712971606No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA3300.22485844139030656No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA3000.20441676490027869No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2910.1982842619532703No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2610.17784258546324247No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA2500.17034730408356558No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2390.16285202270388868No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.1608078550548859No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.15876368740588312No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.1301453403198441No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1910.1301453403198441No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA1800.12265005894016721No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1760.11992450207483016No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1690.11515477756049033No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTT1690.11515477756049033No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA1650.11242922069515328No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1570.10697810696447918No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA1560.10629671774814492No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.1049339393154764No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATCGC200.001836709837.028
GGCTACT200.001836709837.02
CTAGATG250.005480925629.63
GACAAGT250.005480925629.622
CTACGTG250.005480925629.67
ACGTGCG250.005480925629.69
GCGATAT250.005480925629.615
AGGCTAC250.005480925629.61
GGGTGTG358.832423E-426.4285727
CTAACTG358.832423E-426.4285724
AGTTAAT358.832423E-426.42857214
GGTGTGA451.3155438E-424.6666668
TACGGGC451.3155438E-424.6666664
TAGATGT451.3155438E-424.6666664
ATACGGG451.3155438E-424.6666663
GGCGTCG551.8880382E-523.5454548
GACGGGG551.8880382E-523.54545412
CGTGCGC400.001923214223.12510
AGGGTGT400.001923214223.1256
TAACCCG400.001923214223.12529
Produced by FastQC (version 0.11.5)